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Development of Planar Patch-clamp Electrophysiology to Investigate Liposome-based Artificial Nanosensor Devices

Funding: 2005: $33,048
2006: $33,048
2007: $33,048

Project Member(s): Valenzuela, S.

Funding or Partner Organisation: Australian Research Council (ARC Linkage Projects)
Surgical Diagnostics Pty Limited
Ambri Ltd (AMBRI)
Nanion Technologies GmbH

Start year: 2005

Summary: This project aims to characterise the interaction of transport proteins with unsupported lipid bilayer membranes. This will provide the basis for a novel biosensor utilising mechanosensitive ion channels incorporated into an artificial lipid bilayer membrane. To support this outcome, the project will develop the planar patch-clamp electrophysiology recording techniques suitable for liposomes. This provides a significant PhD training opportunity and brings an international focus to the development of planar patch-clamp electrophysiology in Australia. The project has significant commercial potential by developing both the planar patch-clamp electrophysiology techniques for liposomes and producing a novel biomimetic mechanosensitive biosensor.

Publications:

Battle, AR, Valenzuela, S, Mechler, A, Nichols, R, Praporski, S, Di Maio, I, Islam, H, Girard-Egrot, AP, Cornell, BA, Prashar, J, Caruso, F, Martin, LL & Martin, DK 2009, 'Novel engineered ion channel provides controllable ion permability for polyelectrolyte microcapsules coated with a lipid membrane', Advanced Functional Materials, vol. 19, pp. 201-208.
View/Download from: UTS OPUS or Publisher's site

Keywords: nanobiotechnology, mechanosensitive ion channels, lipid bilayer membrane, planar patch clamp electrophysiology,

FOR Codes: Higher education, Scientific instrumentation, Nanotechnology, Biosensor Technologies, Nanotechnology not elsewhere classified, Medical Biotechnology Diagnostics (incl. Biosensors), Scientific Instruments, Learner and Learning Achievement