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A transportable containerised laboratory for rapid cell sorting and high-resolution bioimaging of living aquatic microbes in field locations

Funding: 2013: $580,000

Project Member(s): Doblin, M., Seymour, J., Ralph, P., Vigneswaran, S., Whitchurch, C., Murray, S., Ostrowski, M.

Funding or Partner Organisation: BD Advanced Cytometry
University of Tasmania
Macquarie University
Australian Research Council (ARC Linkage Infrastructure)
Taylor Brothers (Slipway & Engineering) Pty Ltd
University of Western Australia (The University of Western Australia)
University of New South Wales (The University of New South Wales Partnership Fund)

Start year: 2013

Summary: Microbes sustain life on Earth, yet are poorly understood in the marine and aquatic realms due to inadequacies of current field-based methods - researchers must kill or cryopreserve microbes, rather than study living cells. This proposal will overcome this problem by delivering a purpose-built, transportable, live cell analysis laboratory that will house a high-speed BD Influx Mariner rapid sorting flow cytometer and a BD Pathway 855 high-resolution bioimager, to analyse living microbes on location. In an Australian first, this laboratory will facilitate an unprecedented level of discovery of microbial function for leading marine and aquatic researchers, knowledge that is essential to safeguard the health of our planet.

Publications:

Baker, KG, Radford, DT, Evenhuis, C, Kuzhiumparam, U, Ralph, PJ & Doblin, MA 2018, 'Thermal niche evolution of functional traits in a tropical marine phototroph.', Journal of phycology, vol. 54, no. 6, pp. 799-810.
View/Download from: UTS OPUS or Publisher's site

Keywords: microbial ecology,marine biogeochemistry,ecogenomics

FOR Codes: Microbial Ecology, Ecosystem Assessment and Management of Marine Environments, Biological Oceanography, Ecosystem Assessment and Management of Coastal and Estuarine Environments, Marine Flora, Fauna and Biodiversity