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Identification of new Vibrio-specific BioBricks and creation of a temperature-sensitive origin of replication BioBrick for use in Vibrio-specific vectors

Funding or Partner Organisation: Defence Science and Technology Group of the Department of Defence

Start year: 2015

Summary: Vibrio species show a wide range of niche specialization, from free-living forms to those attached to biotic and abiotic surfaces, from symbiotic to pathogenic interactions and from estuarine to deep-sea habitats. They encompass the important human pathogen V. cholerae but are also are common in coastal and estuarine habitats performing important nutrient cycling roles. Most Vibrio species are easily cultured in the laboratory and are subject to numerous genetic techniques thus, making them excellent chassis candidates for synthetic biology approaches. This project has two aims: 1. To identify genes important for environmental survival in Vibrio species that may act as future BioBricks. 2. To develop a new BioBrick encoding a temperature-sensitive origin of replication for deployment in Vibrio species chassis.

Keywords: synthetic biology, vibrio, plasmid, cholera

FOR Codes: Expanding Knowledge in the Biological Sciences, Synthetic Biology, Microbiology, Emerging Defence Technologies