Effect of a Novel Immobilised Antimicrobial Peptide on Bacterial Surfaces

Funding: 2016: $31,788
2017: $33,334
2018: $29,388

Project Member(s): Cranfield, C.

Funding or Partner Organisation: Australian Research Council (ARC Discovery Projects)

Start year: 2016

Summary: The AIM of the project is to determine the mode of action of surface-bound melimine against Gram-negative and Gram-positive bacteria using combinations of state-of-the-art techniques uniquely available to our research group. This aim will be tackled using a variety of techniques including supported lipid bilayers, ; atomic force, confocal, scanning laser and electron microscopy, ; biochemical analyses of cell surfaces; and bacterial mutants to examine the mechanisms involved in its ability to prevent microbial colonisation of surfaces. These strategies have the potential to yield novel antimicrobial coatings for a wide range of applications ranging from biomedical devices to industrial filters. The project has the potential to deliver enormous commercial benefits to Australian industry, and will yield substantial benefits to the Australian economy.

Publications:

Alghalayini, A, Garcia, A, Berry, T & Cranfield, CG 2019, 'The Use of Tethered Bilayer Lipid Membranes to Identify the Mechanisms of Antimicrobial Peptide Interactions with Lipid Bilayers', Antibiotics, vol. 8, no. 1, pp. 12-12.
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Berry, T, Dutta, D, Chen, R, Leong, A, Wang, H, Donald, WA, Parviz, M, Cornell, B, Willcox, M, Kumar, N & Cranfield, CG 2018, 'Lipid Membrane Interactions of the Cationic Antimicrobial Peptide Chimeras Melimine and Cys-Melimine', Langmuir, vol. 34, no. 38, pp. 11586-11592.
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Kuppusamy, R, Yasir, M, Berry, T, Cranfield, CG, Nizalapur, S, Yee, E, Kimyon, O, Taunk, A, Ho, KKK, Cornell, B, Manefield, M, Willcox, M, Black, DS & Kumar, N 2018, 'Design and synthesis of short amphiphilic cationic peptidomimetics based on biphenyl backbone as antibacterial agents', European Journal of Medicinal Chemistry, vol. 143, pp. 1702-1722.
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Nizalapur, S, Kimyon, O, Yee, E, Ho, K, Berry, T, Manefield, M, Cranfield, CG, Willcox, M, Black, DS & Kumar, N 2017, 'Amphipathic guanidine-embedded glyoxamide-based peptidomimetics as novel antibacterial agents and biofilm disruptors', Organic & Biomolecular Chemistry, vol. 15, no. 9, pp. 2033-2051.
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Yu, TT, Nizalapur, S, Ho, KKK, Yee, E, Berry, T, Cranfield, CG, Willcox, M, Black, DS & Kumar, N 2017, 'Design, Synthesis and Biological Evaluation of N‐Sulfonylphenyl glyoxamide‐Based Antimicrobial Peptide Mimics as Novel Antimicrobial Agents', ChemistrySelect, vol. 2, no. 12, pp. 3452-3461.
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Cranfield, CG, Berry, T, Holt, SA, Hossain, KR, Le Brun, AP, Carne, S, Al Khamici, H, Coster, H, Valenzuela, SM & Cornell, B 2016, 'Evidence of the Key Role of H3O+ in Phospholipid Membrane Morphology', LANGMUIR, vol. 32, no. 41, pp. 10725-10734.
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Nizalapur, S, Ho, KKK, Kimyon, O, Yee, E, Berry, T, Manefield, M, Cranfield, CG, Willcox, M, Black, DS & Kumar, N 2016, 'Synthesis and biological evaluation of N-naphthoyl-phenylglyoxamide-based small molecular antimicrobial peptide mimics as novel antimicrobial agents and biofilm inhibitors', ORGANIC & BIOMOLECULAR CHEMISTRY, vol. 14, no. 14, pp. 3623-3637.
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FOR Codes: Biomaterials, Synthesis of Materials, Human Pharmaceutical Treatments (e.g. Antibiotics), Expanding Knowledge in the Biological Sciences, Expanding Knowledge in the Chemical Sciences, Biomaterials , Macromolecular and materials chemistry, Human pharmaceutical treatments