Calibration strategies of metal based contaminants in biological tissue

Project Member(s): Doble, P., Bishop, D.

Funding or Partner Organisation: DAAD Germany (UA DAAD)

Start year: 2016

Summary: Elemental bio-imaging (EBI) involves in situ analysis of elements in biological tissue, and is a field that is constantly improving and expanding as new technology comes onto the market, and new applications realised. One of the fundamental issues with EBI is quantification strategies to determine the levels of metals present in a given sample. To accurately calibrate a technique, preparation of matrix matched standards is necessary. This project aims to use improvements in technology as well as new calibration techniques to identify and quantify nanoparticles in lung tissue. Nanoparticles (NPs) are increasing in use in everyday life, from laundry detergents to cosmetic creams, and there is still large knowledge gaps on the toxicity of different NPs that have been developed for different uses. This project will use EBI to further explore interactions between NPs and the biological system to give us a greater understanding on the physiological effects following exposure.

Publications:

Bishop, DP, Grossgarten, M, Dietrich, D, Vennemann, A, Cole, N, Sperling, M, Wiemann, M, Doble, PA & Karst, U 2018, 'Quantitative imaging of translocated silver following nanoparticle exposure by laser ablation-inductively coupled plasma-mass spectrometry', Analytical Methods, vol. 10, no. 8, pp. 836-840.
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