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Multiplexed Bio-imaging Mass Spectrometry - multiplexed elemental and molecular imaging of the zinc transporter pathway in the ageing mouse brain

Funding: 2017: $137,500
2018: $130,500
2019: $148,000

Project Member(s): Doble, P.

Funding or Partner Organisation: Australian Research Council (ARC Discovery Projects)

Start year: 2017

Summary: This project will develop novel technology for simultaneous 3 D imaging of trace elements and molecules in biological tissues. We will use laser ablation-inductively coupled plasma-mass spectrometry in combination with lanthanide-conjugated probes to map the zinc metabolic pathway in the ageing mouse brain. This project will result in a complete imaging solution for quantitative spatial determination of all important elements, co-factors, metabolites and other functional molecules for investigations of myriad metabolic pathways which are difficult or impossible to track with current technologies. Ultimately this technology will underpin investigations of diseases where trace metal and chemical species dysfunction is implicated.

Publications:

Clases, D, Fingerhut, S, Jeibmann, A, Sperling, M, Doble, P & Karst, U 2019, 'LA-ICP-MS/MS improves limits of detection in elemental bioimaging of gadolinium deposition originating from MRI contrast agents in skin and brain tissues.', Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS), vol. 51, pp. 212-218.
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Clases, D, Gonzalez De Vega, R, Adlard, PA & Doble, PA 2019, 'On-line reverse isotope dilution analysis for spatial quantification of elemental labels used in immunohistochemical assisted imaging mass spectrometry: Via LA-ICP-MS', Journal of Analytical Atomic Spectrometry, vol. 34, no. 2, pp. 407-412.
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Clases, D, Gonzalez de Vega, R, Bishop, D & Doble, P 2019, 'SEC-ICP-MS and on-line isotope dilution analysis for characterisation and quantification of immunochemical assays.', Analytical and bioanalytical chemistry, vol. 411, no. 16, pp. 3553-3560.
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Eijkelkamp, BA, Morey, JR, Neville, SL, Tan, A, Pederick, VG, Cole, N, Singh, PP, Ong, C-LY, Gonzalez de Vega, R, Clases, D, Cunningham, BA, Hughes, CE, Comerford, I, Brazel, EB, Whittall, JJ, Plumptre, CD, McColl, SR, Paton, JC, McEwan, AG, Doble, PA & McDevitt, CA 2019, 'Dietary zinc and the control of Streptococcus pneumoniae infection.', PLoS pathogens, vol. 15, no. 8.
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González de Vega, R, Clases, D, Fernández-Sánchez, ML, Eiró, N, González, LO, Vizoso, FJ, Doble, PA & Sanz-Medel, A 2019, 'MMP-11 as a biomarker for metastatic breast cancer by immunohistochemical-assisted imaging mass spectrometry.', Analytical and bioanalytical chemistry, vol. 411, no. 3, pp. 639-646.
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Pamphlett, R, Kum Jew, S, Doble, PA & Bishop, DP 2019, 'Elemental Analysis of Aging Human Pituitary Glands Implicates Mercury as a Contributor to the Somatopause.', Frontiers in Endocrinology, vol. 10, no. JUN.
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Wenzel, RG, Major, D, Hesp, KF, Hall, E & Doble, P 2019, 'Cobalt accumulation in horses following repeated administration of cobalt chloride', AUSTRALIAN VETERINARY JOURNAL, vol. 97, no. 11, pp. 465-472.
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Bishop, DP, Cole, N, Zhang, T, Doble, PA & Hare, DJ 2018, 'A guide to integrating immunohistochemistry and chemical imaging.', Chemical Society reviews, vol. 47, no. 11, pp. 3770-3787.
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Doble, PA & Miklos, GLG 2018, 'Distributions of manganese in diverse human cancers provide insights into tumour radioresistance.', Metallomics : integrated biometal science, vol. 10, no. 9, pp. 1191-1210.
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Pamphlett, R, Bishop, DP, Kum Jew, S & Doble, PA 2018, 'Age-related accumulation of toxic metals in the human locus ceruleus.', PloS one, vol. 13, no. 9, pp. e0203627-e0203627.
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Keywords: imaging mass spectrometry, ICP-MS, neurodegeneration

FOR Codes: Analytical Spectrometry, Analytical Biochemistry, Expanding Knowledge in the Physical Sciences, Expanding Knowledge in the Biological Sciences