Belli, S, Formenton, A, Noll, T, Ivens, A, Jacquet, R, Desponds, C, Hofer, D & Fasel, N 1999, 'Leishmania major: Histone H1 gene expression from the sw3 locus', EXPERIMENTAL PARASITOLOGY, vol. 91, no. 2, pp. 151-160.
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Cavicchioli, R, Fegatella, F, Ostrowski, M, Eguchi, M & Gottschal, J 1999, 'Sphingomonads from marine environments', JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, vol. 23, no. 4-5, pp. 268-272.
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Christen, G, Stevens, G, Lukins, PB, Renger, G & Larkum, AWD 1999, 'Isolation and characterisation of oxygen evolving thylakoids from the marine prokaryote Prochloron didemni', FEBS Letters, vol. 449, no. 2-3, pp. 264-268.
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The present study describes the first successful attempt to isolate oxygen evolving thylakoids and thylakoid fragments from the marine prokaryote Prochloron didemni, a member of the recently discovered group of prochlorophytes. Oxygen evolving thylakoid membranes and fragments were isolated from seawater suspended cells of Prochloron didemni by passage of the cells through a Yeda press and subsequent differential centrifugation of the broken material. Three fractions were collected at 1000xg, 5000xg, and 30 000xg and identified by light microscopy as cells (and their fragments), thylakoids and membrane fragments, respectively. Pigment content, oxygen evolution rate and 77 K fluorescence spectra of these fractions were virtually identical. This finding indicates that the membrane fragments obtained are not enriched in photosystem II. The P680(+.) reduction kinetics of thylakoid membrane fragments were determined by monitoring flash induced absorption changes at 830 nm and analysing the time course of their decay. The multiphasic relaxation kinetics and their modification by NH2OH were found to be similar to those observed in cyanobacteria and plants. These findings provide an independent line of evidence for the idea of a high conservation of the basic structural and functional pattern of the water oxidising complex in all organisms that perform oxygenic photosynthesis. Copyright (C) 1999 Federation of European Biochemical Societies.
De Lucca, S, Sporik, R, O’Meara, TJ & Tovey, ER 1999, 'Mite allergen (Der p 1) is not only carried on mite feces', Journal of Allergy and Clinical Immunology, vol. 103, no. 1, pp. 174-175.
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De Lucca, SD, Taylor, DJM, O'Meara, TJ, Jones, AS & Tovey, ER 1999, 'Measurement and characterization of cockroach allergens detected during normal domestic activity', Journal of Allergy and Clinical Immunology, vol. 104, no. 3 II, pp. 672-680.
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Background: Cockroach allergen is recognized as a causal factor for asthma. However, airborne cockroach allergen has not been detected in undisturbed conditions, and therefore the behavior and properties of airborne cockroach allergen have been poorly characterized. A new aeroallergen sampling method and sensitive system of immunoassay have been used to examine cockroach allergen exposure. Objective: Our purpose was to measure and characterize airborne cockroach allergens during normal domestic exposure in the homes of Sydney, Australia. Methods: Air sampling with Institute of Occupational Medicine, Edinburgh (IOM) samplers was performed in the living rooms of 10 houses during low- and no-disturbance environments. In addition, inhaled particles were collected by each home occupant during low domestic exposure with use of intra-nasal samplers that impact particles onto an adhesive surface. The particles collected on the IOMs and the intranasal samplers were immunostained with Bla g 1 monoclonal antibodies. Particle size, morphologic characteristics, and the relative Bla g 1 content of particles were estimated. Reservoir dust samples from the kitchen, living room, and bedroom were assayed by an ELISA. Two forms of repeatability of IOM air sampling were examined. The first measure tested the repeatability of 2 IOM samples collected simultaneously in the same room during low- and no-disturbance activities. The second measure examined the repeatability of IOM sampling over time on 10 consecutive days. Results: Bla g 1 was detected in reservoir dust samples taken from all homes (geometric mean 1.5 U/g, range 0.2-9.4 U/g). Inhaled particles containing Bla g 1 were detected during 1 hour of intra-nasal sampling in 8 of 10 homes during low disturbance. Cockroach particles were detected on all of the IOM samples collected for both 4-hour low-disturbance and overnight no-disturbance sampling environments. Particles containing Bla g 1 collected with the IOM samplers during l...
Doblin, MA, Blackburn, S & Hallegraeff, GM 1999, 'Comparative study of selenium requirements of three phytoplankton species: Gymnodinium catenatum, Alexandrium minutum (Dinophyta) and Chaetoceros cf. tenuissimus (Bacillariophyta)', Journal Of Plankton Research, vol. 21, no. 6, pp. 1153-1169.
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This study investigated the selenium (Se) requirements of three phytoplankton species which commonly bloom in southern Australian estuaries. The present study showed that the toxic dinoflagellate Gymnodinium catenatum Graham had an obligate requirement f
Doblin, MA, Blackburn, S & Hallegraeff, GM 1999, 'Growth and biomass stimulation of the toxic dinoflagellate Gymnodinium catenatum (Graham) by dissolved organic substances', Journal Of Experimental Marine Biology And Ecology, vol. 236, no. 1, pp. 33-47.
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Blooms of the toxic dinoflagellate Gymnodinium catenatum are annually recurrent events in south-east Tasmanian waters. Extensive blooms are preceded by a rainfall trigger and the associated influx of dissolved organic matter (DOM; otherwise known as humi
Field, CD 1999, 'Mangrove rehabilitation: Choice and necessity', Hydrobiologia, vol. 413, pp. 47-52.
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The concept of mangrove ecosystem rehabilitation is considered. Four main reasons for rehabilitating mangroves are identified: conservation, landscaping, sustainable production and coastal protection. Practical aspects of mangrove rehabilitation, such as the causes of site degradation, site selection, source of seedlings and planting, monitoring and maintenance are then briefly mentioned. Future developments that may impact on mangrove ecosystem restoration such as the importance of biodiversity, biotechnology, ecological modelling, mapping, human ecology and data bases, are then briefly reviewed. Finally, the matter of choice and necessity is addressed.
Field, CD 1999, 'Rehabilitation of mangrove ecosystems: An overview', Marine Pollution Bulletin, vol. 37, no. 8-12, pp. 383-392.
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The concept and goals of mangrove ecosystem rehabilitation are considered and contrasted with ideas of ecosystem restoration. Three reasons for mangrove rehabilitation: conservation and landscaping; multiple use systems for high sustainable yield and protection of coastal areas, are then examined in detail. In each case, the underlying philosophy and limitations are presented. The practical problems of site selection for mangrove planting and techniques for regenerating mangroves are then considered. Some comments and data are then offered on mangrove ecosystem rehabilitation that is being carried out world-wide. Comment is made on the paucity of information. The practice and importance of monitoring and maintaining rehabilitated mangrove ecosystems is then presented. Finally, there is a discussion on the future management and research needs of mangrove ecosystem rehabilitation.
Hawes, I, Smith, R & Sutherland, DL 1999, 'Development of microbial mats on contaminated soils from the former site of Vanda Station, Antarctica', New Zealand Natural Sciences, vol. 24, pp. 53-68.
Helfrich, M, Ross, A, King, GC, Turner, AG & Larkum, AWD 1999, 'Identification of [8-vinyl]-protochlorophyllide a in phototrophic prokaryotes and algae: Chemical and spectroscopic properties', Biochimica et Biophysica Acta - Bioenergetics, vol. 1410, no. 3, pp. 262-272.
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[8-vinyl]-Protochlorophyllide a1 was isolated from a Prochloron sp. associated with the host ascidian, Lissoclinum patella. To obtain sufficient amounts for identification of the purified pigment, suitable extraction procedures and HPLC systems were developed. The structure was finally elucidated by UV-VIS and fluorescence spectroscopy, mass spectrometry and NMR (rotating-frame Overhauser enhancement spectroscopy). [8-vinyl]-Protochlorophyllide a was originally detected only as an intermediate in chlorophyll biosynthesis. Although its presence as a light-harvesting pigment was previously suggested in some prochlorophytes and eukaryotic algae, this is the first unequivocal demonstration of [8-vinyl]-protochlorophyllide a in an oxygenic phototroph. We also show that [8-vinyl]-protochlorophyllide a occurs in Prochloron species of four other ascidians as well as in Micromonas pusilla and Prochlorococcus marinus. The possible role of this pigment in photosynthesis is discussed. Copyright (C) 1999 Elsevier Science B.V.
Lockhart, PJ, Howe, CJ, Barbrook, AC, Larkum, AWD & Penny, D 1999, 'Spectral analysis, systematic bias, and the evolution of chloroplasts [3]', Molecular Biology and Evolution, vol. 16, no. 4, pp. 573-576.
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Poulos, O'Meara, Sporik & Tovey 1999, 'Detection of inhaled Der p 1', Clinical Experimental Allergy, vol. 29, no. 9, pp. 1232-1238.
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Ralph, PJ 1999, 'Light-induced photoinhibitory stress responses of laboratory-cultured Halophila ovalis', Botanica Marina, vol. 42, no. 1, pp. 11-22.
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This paper details experiments performed to investigate the short-term stress effects of both high and low-light regimes on laboratory-cultured Halophila ovalis using chlorophyll fluorescence. Increasing irradiance up to 400 mu mol quanta m(-2) s(-1) on
Ralph, PJ 1999, 'Photosynthetic response of Halophila ovalis (R-Br.) Hook. f. to combined environmental stress', Aquatic Botany, vol. 65, no. 1-Apr, pp. 83-96.
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Combinations of stresses showed an additive effect in comparison to the individual stress responses. It is apparent from these results that thermal, elevated-light or osmotic stress increases the sensitivity of Halophila ovalis to any of the other stress
Ralph, PJ, Gademann, R, Larkum, A & Schreiber, U 1999, 'In situ underwater measurements of photosynthetic activity of coral zooxanthellae and other reef-dwelling dinoflagellate endosymbionts', Marine Ecology-Progress Series, vol. 180, pp. 139-147.
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Chlorophyll fluorescence was used to assess the iu situ photosynthesis of a range of reef-dwelling endosymbionts. Such non-intrusive in situ measurements became possible after the recent development of a submersible pulse modulated fluorometer (DIVING-PA