Cranfield, CG, Wood, AW, Anderson, V & Menezes, KG 2001, 'Effects of mobile phone type signals on calcium levels within human leukaemic T-cells (Jurkat cells)', INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, vol. 77, no. 12, pp. 1207-1217.
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Harrop, SJ, DeMaere, MZ, Fairlie, WD, Reztsova, T, Valenzuela, SM, Mazzanti, M, Tonini, R, Qiu, MR, Jankova, L, Warton, K, Bauskin, AR, Wu, WM, Pankhurst, S, Campbell, TJ, Breit, SN & Curmi, PMG 2001, 'Crystal Structure of a Soluble Form of the Intracellular Chloride Ion Channel CLIC1 (NCC27) at 1.4-Å Resolution', Journal of Biological Chemistry, vol. 276, no. 48, pp. 44993-45000.
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CLIC1 (NCC27) is a member of the highly conserved class of chloride ion channels that exists in both soluble and integral membrane forms. Purified CLIC1 can integrate into synthetic lipid bilayers forming a chloride channel with similar properties to those observed in vivo. The structure of the soluble form of CLIC1 has been determined at 1.4-Angstrom resolution. The protein is monomeric and structurally homologous to the glutathione S-transferase superfamily, and it has a redox-active site resembling glutaredoxin. The structure of the complex of CLIC1 with glutathione shows that glutathione occupies the redox-active site, which is adjacent to an open, elongated slot lined by basic residues. Integration of CLIC1 into the membrane is likely to require a major structural rearrangement, probably of the N-domain (residues 1-90), with the putative transmembrane helix arising from residues in the vicinity of the redox-active site. The structure indicates that CLIC1 is likely to be controlled by redox-dependent processes.
Mazzanti, M, Valenzuela, S, Tonini, R, Qui, MR, Warton, K, Musgrove, E, Campbell, TJ & Fairle, D 2001, 'The Nuclear Chloride Ion Channel Ncc27 Is Involved In Regulation Of The Cell Cycle', Biophysical Journal, vol. 80, no. 1, pp. 1-1.
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NA
Raftery, MJ, Yang, Z, Valenzuela, SM & Geczy, CL 2001, 'Novel Intra- and Inter-molecular Sulfinamide Bonds in S100A8 Produced by Hypochlorite Oxidation', Journal of Biological Chemistry, vol. 276, no. 36, pp. 33393-33401.
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Hypochlorite is a major oxidant generated when neutrophils and macrophages are activated at inflammatory sites, such as in atherosclerotic lesions. Murine S100A8 (A8) is a major cytoplasmic protein in neutrophils and is secreted by macrophages in response to inflammatory stimuli. After incubation with reagent HOCl for 10 min, ~85% of A8 was converted to 4 oxidation products, with electrospay ionization mass spectrometry masses of m/z 10354, 10388, 10354 ± 1, and 20707 ± 3. All were resistant to reduction by dithiothreitol. Initial formation of a reactive Cys sulfenic acid intermediate was demonstrated by the rapid conjugation of 5,5-dimethyl-1,3-cyclohexanedione (dimedone) to HOCl-treated A8 to form stable adducts. Matrix-assisted laser desorption-reflectron time of flight peptide mass fingerprinting of isolated oxidation products confirmed the mass additions observed in the full-length proteins. Both Met36/73 were converted to Met36/73 sulfoxides. An additional product with an unusual mass addition of m/z 14 (±0.2) was identified and corresponded to the addition of oxygen to Cys41, conjugation to various epsilon -amines of Lys6, Lys34/35, or Lys87 with loss of dihydrogen and formation of stable intra- or inter-molecular sulfinamide cross-links. Specific fragmentations identified in matrix-assisted laser desorption-post source decay spectra and low energy collisional-induced dissociation tandem mass spectroscopy spectra of sulfinamide-containing digest peptides confirmed Lys34/35 to Cys41 sulfinamide bonds. HOCl oxidation of mutants lacking Cys41 (Ala41S100A8) or specific Lys residues (e.g. Lys34/35, Ala34/35S100A8) did not form sulfinamide cross-links. HOCl generated by myeloperoxidase and H2O2 and by phorbol 12-myristate 13-acetate-activated neutrophils also formed these products.
Tie, H, Walker, BD, Singleton, CB, Bursill, JA, Wyse, KR, Campbell, TJ, Valenzuela, SM & Breit, SN 2001, 'Clozapine and Sudden Death', Journal of Clinical Psychopharmacology, vol. 21, no. 6, pp. 630-632.
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Walker, BD, Tie, H, Singleton, C, Bursill, J, Wyse, KR, Bauskin, AR, Valenzuela, S, Wu, W, Breit, SN & Campbell, TJ 2001, 'Modification Of Herg Channel Properties Under Conditions Of Simulated Myocardial Ischemia', Journal Of The American College Of Cardiology, vol. 37, no. 2, pp. 1-2.
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