Cai, H, An, X, Cui, J, Li, J, Wen, S, Li, K, Shen, M, Zheng, L, Zhang, G & Shi, X 2013, 'Facile Hydrothermal Synthesis and Surface Functionalization of Polyethyleneimine-Coated Iron Oxide Nanoparticles for Biomedical Applications', ACS Applied Materials & Interfaces, vol. 5, no. 5, pp. 1722-1731.
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Chen, Q, Li, K, Wen, S, Liu, H, Peng, C, Cai, H, Shen, M, Zhang, G & Shi, X 2013, 'Targeted CT/MR dual mode imaging of tumors using multifunctional dendrimer-entrapped gold nanoparticles', Biomaterials, vol. 34, no. 21, pp. 5200-5209.
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de Sterke, CM, Kabakova, IV, Uddin, I, Jeyaratnam, J & Malomed, BA 2013, 'Spontaneous symmetry breaking in a double-defect nonlinear grating', Physical Review A, vol. 88, no. 3.
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Ding, Y, Zhang, Y, Peng, T, Lu, Y, Jin, D, Ren, Q, Liu, Y, Han, J & Xi, P 2013, 'Observation of mesenteric microcirculatory disturbance in rat by laser oblique scanning optical microscopy', SCIENTIFIC REPORTS, vol. 3.
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Ischemia-reperfusion (I/R) injury model has been widely applied to the study of microcirculation disturbance. In this work, we used laser oblique scanning optical microscopy (LOSOM) to observe the microcirculation system in the mesentery of rat model. Utilizing a localized point-scanning detection scheme, high-contrast images of leukocytes were obtained. The extended detection capability facilitated both the automatic in vivo cell counting and the accurate measurement of the rolling velocity of leukocytes. Statistical analysis of the different treatment groups suggested that the distinction between I/R and sham groups with time lapse is significant.
Eilenberger, F, Kabakova, IV, de Sterke, CM, Eggleton, BJ & Pertsch, T 2013, 'Cavity Optical Pulse Extraction: ultra-short pulse generation as seeded Hawking radiation', Scientific Reports, vol. 3, no. 1.
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Gupta, B, Zhu, Y, Guan, B, Reece, PJ & Gooding, JJ 2013, 'Functionalised porous silicon as a biosensor: emphasis on monitoring cells in vivo and in vitro', The Analyst, vol. 138, no. 13, pp. 3593-3593.
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Hou, HW, Warkiani, ME, Khoo, BL, Li, ZR, Soo, RA, Tan, DS-W, Lim, W-T, Han, J, Bhagat, AAS & Lim, CT 2013, 'Isolation and retrieval of circulating tumor cells using centrifugal forces', Scientific Reports, vol. 3, no. 1, p. 1259.
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Presence and frequency of rare circulating tumor cells (CTCs) in bloodstreams of cancer patients are pivotal to early cancer detection and treatment monitoring. Here, we use a spiral microchannel with inherent centrifugal forces for continuous, size-based separation of CTCs from blood (Dean Flow Fractionation (DFF)) which facilitates easy coupling with conventional downstream biological assays. Device performance was optimized using cancer cell lines (> 85% recovery), followed by clinical validation with positive CTCs enumeration in all samples from patients with metastatic lung cancer (n = 20; 5-88 CTCs per mL). The presence of CD133⁺ cells, a phenotypic marker characteristic of stem-like behavior in lung cancer cells was also identified in the isolated subpopulation of CTCs. The spiral biochip identifies and addresses key challenges of the next generation CTCs isolation assay including antibody independent isolation, high sensitivity and throughput (3 mL/hr); and single-step retrieval of viable CTCs.
Kabakova, IV, Pant, R, Choi, D-Y, Debbarma, S, Luther-Davies, B, Madden, SJ & Eggleton, BJ 2013, 'Narrow linewidth Brillouin laser based on chalcogenide photonic chip', OPTICS LETTERS, vol. 38, no. 17, pp. 3208-3211.
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We present the first demonstration of a narrow linewidth, waveguide-basedBrillouin laser which is enabled by large Brillouin gain of a chalcogenidechip. The waveguides are equipped with vertical tapers for low loss coupling.Due to optical feedback for the Stokes wave, the lasing threshold is reduced to360 mW, which is 5 times lower than the calculated single-pass Brillouinthreshold for the same waveguide. The slope efficiency of the laser is found tobe 30% and the linewidth of 100 kHz is measured using a self-heterodyne method.
Li, K, Wen, Larson, AC, Zhang, Z, Shen, Chen, Shi, X & Zhang 2013, 'Multifunctional dendrimer-based nanoparticles for in vivo MR/CT dual-modal molecular imaging of breast cancer', International Journal of Nanomedicine, vol. 8, pp. 2589-2589.
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Liao, J, Yang, Z, Wu, H, Yan, D, Qiu, J, Song, Z, Yang, Y, Zhou, D & Yin, Z 2013, 'Enhancement of the up-conversion luminescence of Yb3+/Er3+ or Yb3+/Tm3+ co-doped NaYF4 nanoparticles by photonic crystals', Journal of Materials Chemistry C, vol. 1, no. 40, pp. 6541-6541.
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A new method for enhancing the upconversion (UC) emission of rare-earth doped nanoparticles is reported, in which Yb3+/Er3+ or Yb3+/Tm3+ co-doped NaYF4 nanoparticles are deposited on to the surface of photonic crystal (PC) films. The UC emission of the Yb3+/Er3+ or Yb3+/Tm3+ co-doped NaYF4 nanoparticles on the PC surface was notably enhanced when the UC emission bands of the Yb3+/Er3+ or Yb 3+/Tm3+ co-doped NaYF4 nanoparticles were within the range of the photonic band gap of the PCs, indicating that the PCs were efficient and selective reflection mirrors. The results show that PCs may have potential applications in UC optoelectronics and lighting devices. © 2013 The Royal Society of Chemistry.
Lin, G, Baraban, L, Han, L, Karnaushenko, D, Makarov, D, Cuniberti, G & Schmidt, OG 2013, 'Magnetoresistive Emulsion Analyzer', Scientific Reports, vol. 3, no. 1.
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Liu, H, Xu, Y, Wen, S, Chen, Q, Zheng, L, Shen, M, Zhao, J, Zhang, G & Shi, X 2013, 'Targeted Tumor Computed Tomography Imaging Using Low‐Generation Dendrimer‐Stabilized Gold Nanoparticles', Chemistry – A European Journal, vol. 19, no. 20, pp. 6409-6416.
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AbstractWe report a facile approach to fabricating low‐generation poly(amidoamine) (PAMAM) dendrimer‐stabilized gold nanoparticles (Au DSNPs) functionalized with folic acid (FA) for in vitro and in vivo targeted computed tomography (CT) imaging of cancer cells. In this study, amine‐terminated generation 2 PAMAM dendrimers were employed as stabilizers to form Au DSNPs without additional reducing agents. The formed Au DSNPs with an Au core size of 5.5 nm were covalently modified with the targeting ligand FA, followed by acetylation of the remaining dendrimer terminal amines to endow the particles with targeting specificity and improved biocompatibility. Our characterization data show that the formed FA‐modified Au DSNPs are stable at different pH values (5—8) and temperatures (4–50 °C), as well as in different aqueous media. MTT assay data along with cell morphology observations reveal that the FA‐modified Au DSNPs are noncytotoxic in the particle concentration range of 0–3000 nM. X‐ray attenuation coefficient measurements show that the CT value of FA‐modified Au DSNPs is much higher than that of Omnipaque (a clinically used CT contrast agent) at the same concentration of the radiodense elements (Au or iodine). Importantly, the FA‐modified Au DSNPs are able to specifically target a model cancer cell line (KB cells, a human epithelial carcinoma cell line) over‐expressing FA receptors and they enable targeted CT imaging of the cancer cells in vitro and the xenografted tumor model in vivo after intravenous administration of the particles. With the simple synthesis approach, easy modification, good cytocompatibility, and high X‐ray attenuation coefficient, the FA‐modified low‐generation Au DSNPs could be used as promising contrast agents for targeted CT imaging of different tumors over‐expressing FA receptors.
Liu, H, Xu, Y, Wen, S, Zhu, J, Zheng, L, Shen, M, Zhao, J, Zhang, G & Shi, X 2013, 'Facile hydrothermal synthesis of low generation dendrimer-stabilized gold nanoparticles for in vivo computed tomography imaging applications', Polymer Chemistry, vol. 4, no. 6, pp. 1788-1788.
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Lu, J, Paulsen, IT & Jin, D 2013, 'Application of Exonuclease III-Aided Target Recycling in Flow Cytometry: DNA Detection Sensitivity Enhanced by Orders of Magnitude', Analytical Chemistry, vol. 85, no. 17, pp. 8240-8245.
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DNA-functionalized microspheres in conjugation with flow cytometry detection are widely used for high-throughput nucleic acid assays. Although such assays are rapid and capable of simultaneous analysis of multiple nucleic acid analytes in a single test, the intrinsic limitation in sensitivity remains challenging. Here we report a simple, highly sensitive, and reproducible method based on Exonuclease III-aided target recycling technique applied for DNA quantification in flow cytometry. By loading a high density of Cy5-labeled probe DNA on microspheres (15 μm), we achieved hitherto unreported DNA detection limit of 3.2 pM in flow cytometry bead assay, enhancing the sensitivity by a factor of over 56.8 compared to the conventional direct hybridization bead assay. Furthermore, we evaluated multiplexing capability by simultaneous detections of two target DNAs with FAM and Cy5 reporter conjugated probes. Therefore, the novel Exonuclease III-amplified flow cytometry bead assay has great potential for the rapid, sensitive, and accurate detection and quantification of nucleic acids in clinical diagnosis and biomedical research. © 2013 American Chemical Society.
Malaei, F, Hesaraki, M, Saadati, M, Ahdi, AM, Sadraeian, M, Honari, H & Nazarian, S 2013, 'Immunogenicity of a new recombinant IpaC from Shigella dysenteriae type I in guinea pig as a vaccine candidate.', Iran J Immunol, vol. 10, no. 2, pp. 110-117.
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BACKGROUND: Recombinant vaccine technology is one of the most developed means in controlling infectious diseases. However, an effective vaccine against Shigella is still missing. OBJECTIVE: To evaluate recombinant IpaC protein of Shigella as a vaccine candidate. METHODS: In this study we cloned IpaC gene into an expression vector in prokaryotic system. The protein expression was evaluated by SDS-PAGE and Western-Blotting analysis. The recombinant protein was purified using Ni-NTA affinity chromatography. Guinea pigs were immunized with the recombinant protein and the level of immunogenicity was examined by ELISA and Western blotting of IpaC. Challenge test was done through the intraoculary injection of Shigella dysenteriae (6×108 CFU/eye) and after 48 hours was scored for keratoconjunctivitis. RESULTS: The results showed a remarkable level of immunogenicity in terms of antibody response and protection against keratoconjunctivitis in tested animals. The recombinant IpaC protein provided a protective system against Shigella dysenteriae type I during the challenge test. CONCLUSION: The results showed the potential of using recombinant IpaC in preparation of vaccine in perspective studies.
McClements, L, Yakkundi, A, Papaspyropoulos, A, Harrison, H, Ablett, MP, Jithesh, PV, McKeen, HD, Bennett, R, Donley, C, Kissenpfennig, A, McIntosh, S, McCarthy, HO, O'Neill, E, Clarke, RB & Robson, T 2013, 'Targeting Treatment-Resistant Breast Cancer Stem Cells with FKBPL and Its Peptide Derivative, AD-01, via the CD44 Pathway', Clinical Cancer Research, vol. 19, no. 14, pp. 3881-3893.
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AbstractPurpose: FK506-binding protein like (FKBPL) and its peptide derivative, AD-01, have already shown tumor growth inhibition and CD44-dependent antiangiogenic activity. Here, we explore the ability of AD-01 to target CD44-positive breast cancer stem cells (BCSC).Experimental Design: Mammosphere assays and flow cytometry were used to analyze the effect of FKBPL overexpression/knockdown and AD-01 treatment ± other anticancer agents on BCSCs using breast cancer cell lines (MCF-7/MDA-231/ZR-75), primary patient samples, and xenografts. Delays in tumor initiation were evaluated in vivo. The anti–stem cell mechanisms were determined using clonogenic assays, quantitative PCR (qPCR), and immunofluorescence.Results: AD-01 treatment was highly effective at inhibiting the BCSC population by reducing mammosphere-forming efficiency and ESA+/CD44+/CD24− or aldehyde dehydrogenase (ALDH)+ cell subpopulations in vitro and tumor initiation in vivo. The ability of AD-01 to inhibit the self-renewal capacity of BCSCs was confirmed; mammospheres were completely eradicated by the third generation. The mechanism seems to be due to AD-01–mediated BCSC differentiation shown by a significant decrease in the number of holoclones and an associated increase in meroclones/paraclones; the stem cell markers, Nanog, Oct4, and Sox2, were also significantly reduced. Furthermore, we showed additive inhibitory effects when AD-01 was combined with the Notch inhibitor, DAPT. AD-01 was also able to abrogate a chemo- and radiotherapy-induced enrichment in BCSCs. Finally, FKBPL knockdown led to an increase in Nanog/Oct4/Sox2 and an increase in BCSCs, highlighting a role for endogenous FKBPL in stem cell signaling.Conclusions: AD-01 has dual antiangiogenic and anti-BCSC activity, which will be advantageous as this agent enters clinical trial. Clin Cancer Res; 19(14); 3881–93. ©2013 AACR.
Ngalim, SH, Magenau, A, Zhu, Y, Tønnesen, L, Fairjones, Z, Gooding, JJ, Böcking, T & Gaus, K 2013, 'Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy', Journal of Visualized Experiments, no. 74.
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Rong, Y, Wu, W, Ni, X, Kuang, T, Jin, D, Wang, D & Lou, W 2013, 'Lactate dehydrogenase A is overexpressed in pancreatic cancer and promotes the growth of pancreatic cancer cells', Tumor Biology, vol. 34, no. 3, pp. 1523-1530.
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Sadraeian, M, Ghoshoon, MB, Mohkam, M, Karimi, Z, Rasoul-Amini, S & Ghasemi, Y 2013, 'Modification in media composition to obtain secretory production of STxB-based vaccines using Escherichia coli', Virologica Sinica, vol. 28, no. 1, pp. 43-48.
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Sadraeian, M, Khoshnood Mansoorkhani, MJ, Mohkam, M, Rasoul-Amini, S, Hesaraki, M & Ghasemi, Y 2013, 'Prevention and Inhibition of TC-1 Cell Growth in Tumor Bearing Mice by HPV16 E7 Protein in Fusion with Shiga Toxin B-Subunit from shigella dysenteriae.', Cell J, vol. 15, no. 2, pp. 176-181.
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OBJECTIVE: For immunotherapy of human papillomavirus (HPV) -16-associated cervical cancers the E7 protein is considered a prime candidate. However it is a poor inducer of cytotoxic T-cell response, when being used as a singular antigen in protein vaccination. Hence, in this study we focused on the utilization of a vaccine delivery system for prevention or treatment of cervical cancer. MATERIALS AND METHODS: In this experimental study, we designed and evaluated a novel fusion protein comprising HPV16 E7 antigen fused to Shiga toxin B-subunit (STxB) as both an antigen vector and an adjuvant. Then we designed two preventive and therapeutic tumor models to investigate the prevention and inhibition of TC-1 cell growth in female C57BL/6 mice, respectively. In each model, mice were immunized with the recombinant protein of E7-STxB or E7 without any adjuvant. RESULTS: We demonstrated that prophylactic immunization of E7-STxB protected mice against TC-1 cells. Also in the therapeutic model, E7-STxB inhibited TC-1 tumor growth inlungs. The results were significant when compared with the immunization of E7 singularly. CONCLUSION: We concluded that immunization with the E7-STxB protein without any adjuvant could generate anti-tumor effect in mice challenged with TC-1 cells.This research verifies the clinical applications and the future prospects of developing HPV16 E7 therapeutic vaccines fused to immunoadjuvants.
Sadraeian, M, Rasoul-Amini, S, Mansoorkhani, MJK, Mohkam, M, Ghoshoon, MB & Ghasemi, Y 2013, 'Induction of Antitumor Immunity Against Cervical Cancer by Protein HPV-16 E7 in Fusion With Ricin B Chain in Tumor-Bearing Mice', International Journal of Gynecologic Cancer, vol. 23, no. 5, pp. 809-814.
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ObjectiveIn immunotherapy of HPV-16–associated cervical cancers, the E7 protein is considered as a prime candidate. However, it is a poor inducer of a cytotoxic T-cell response when used as a singular antigen in protein vaccination. Therefore, to design effective cancer vaccines, the best tumor antigens should be combined with the most effective immunogens or drug delivery tools to achieve positive clinical results. In this study, we fused HPV-16 E7 with the lectin subunit of ricin toxin (RTB) from castor plant as a vaccine adjuvant/carrier.Materials and MethodsAfter reaching the soluble form of the recombinant protein, we designed 2 preventive and inhibition tumor models for investigation of the prevention and rejection of TC-1 cell growth in female C57BL/6 mice, respectively. In each model, mice were immunized with the recombinant protein of E7-RTB or E7 without any adjuvant.ResultsWe demonstrated that prophylactic immunization of E7-RTB protected mice against challenge from TC-1 cells. Also in the therapeutic model, E7-RTB could inhibit TC-1 tumor growth in the lung. The results were significant compared with the immunization of E7 singularly.ConclusionsWe concluded that immunization with E7-RTB protein without any adjuvant could generate antitumor effects in mice challenged with TC-1 cells. This research verifies the clinical applications and the future prospects for development of HPV-16 E7 therapeutic vaccines fused to immunoadjuvants.
Wang, S, Wu, Y, Guo, R, Huang, Y, Wen, S, Shen, M, Wang, J & Shi, X 2013, 'Laponite Nanodisks as an Efficient Platform for Doxorubicin Delivery to Cancer Cells', Langmuir, vol. 29, no. 16, pp. 5030-5036.
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Warkiani, ME, Bhagat, AAS, Khoo, BL, Han, J, Lim, CT, Gong, HQ & Fane, AG 2013, 'Isoporous Micro/Nanoengineered Membranes', ACS Nano, vol. 7, no. 3, pp. 1882-1904.
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Watts, AL, Singh, N, Poulton, CG, Magi, EC, Kabakova, IV, Hudson, DD & Eggleton, BJ 2013, 'Photoinduced axial quantization in chalcogenide microfiber resonators', Journal of the Optical Society of America B, vol. 30, no. 12, pp. 3249-3249.
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We investigate axial quantization in chalcogenide (As2S3) whispering gallery mode microfiber resonators. A microcavity is fabricated using a positive photoinduced index perturbation in the microfiber, and the modes are excited through evanescent field coupling with a tapered silica fiber. We show that the modes of the unperturbed fiber split into ladders of modes due to the confinement along the axial direction of the fiber. The axial quantization of the modes is reproduced with a combination of numerical models. Due to the high nonlinearity and photosensitive properties of chalcogenide glasses, microcavities in these materials offer unique potential in nonlinear optics and sensing applications. © 2013 Optical Society of America.
Wen, S, Li, K, Cai, H, Chen, Q, Shen, M, Huang, Y, Peng, C, Hou, W, Zhu, M, Zhang, G & Shi, X 2013, 'Multifunctional dendrimer-entrapped gold nanoparticles for dual mode CT/MR imaging applications', Biomaterials, vol. 34, no. 5, pp. 1570-1580.
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Wen, S, Liu, H, Cai, H, Shen, M & Shi, X 2013, 'Targeted and pH‐Responsive Delivery of Doxorubicin to Cancer Cells Using Multifunctional Dendrimer‐Modified Multi‐Walled Carbon Nanotubes', Advanced Healthcare Materials, vol. 2, no. 9, pp. 1267-1276.
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AbstractWe report the use of multifunctional dendrimer‐modified multi‐walled carbon nanotubes (MWCNTs) for targeted and pH‐responsive delivery of doxorubicin (DOX) into cancer cells. In this study, amine‐terminated generation 5 poly(amidoamine) (PAMAM) dendrimers modified with fluorescein isothiocyanate (FI) and folic acid (FA) were covalently linked to acid‐treated MWCNTs, followed by acetylation of the remaining dendrimer terminal amines to neutralize the positive surface potential. The formed multifunctional MWCNTs (MWCNT/G5.NHAc‐FI‐FA) were characterized via different techniques. Then, the MWCNT/G5.NHAc‐FI‐FA was used to load DOX for targeted and pH‐responsive delivery to cancer cells overexpressing high‐affinity folic acid receptors (FAR). We showed that the MWCNT/G5.NHAc‐FI‐FA enabled a high drug payload and encapsulation efficiency both up to 97.8% and the formed DOX/MWCNT/G5.NHAc‐FI‐FA complexes displayed a pH‐responsive release property with fast DOX release under acidic environment and slow release at physiological pH conditions. Importantly, the DOX/MWCNT/G5.NHAc‐FI‐FA complexes displayed effective therapeutic efficacy, similar to that of free DOX, and were able to target to cancer cells overexpressing high‐affinity FAR and effectively inhibit the growth of the cancer cells. The synthesized multifunctional dendrimer‐modified MWCNTs may be used as a targeted and pH‐responsive delivery system for targeting therapy of different types of cancer cells.
Wen, S, Zheng, F, Shen, M & Shi, X 2013, 'Surface modification and PEGylation of branched polyethyleneimine for improved biocompatibility', Journal of Applied Polymer Science, vol. 128, no. 6, pp. 3807-3813.
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AbstractIn this article, we report the surface modification of branched polyethyleneimine (PEI) for improved biocompatibility. PEIs with different surface functionalities were synthesized via covalent modification of the PEI amines, including neutralized PEI modified with acetic anhydride, negatively charged PEI modified with succinic anhydride, hydroxylated PEI modified with glycidol, and PEI–poly(ethylene glycol) (PEG) conjugates modified with both PEG and acetic anhydride. The modified PEI derivatives were characterized with 1H‐NMR, Fourier transform infrared spectroscopy, and ζ‐potential measurements. An in vitro cytotoxicity assay of mouse fibroblasts revealed that the biocompatibility of PEI was significantly improved after these modifications. The neutral and negatively charged PEIs were nontoxic at concentrations up to 200 μg/mL, whereas the pristine PEI was toxic to cells at concentrations as low as 10 μg/mL. The successfully modified PEIs with different surface charges and functionalities may provide a range of opportunities for various biomedical applications. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
Wen, S, Zheng, F, Shen, M & Shi, X 2013, 'Synthesis of polyethyleneimine-stabilized gold nanoparticles for colorimetric sensing of heparin', Colloids and Surfaces A: Physicochemical and Engineering Aspects, vol. 419, pp. 80-86.
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Winful, HG, Kabakova, IV & Eggleton, BJ 2013, 'Model for distributed feedback Brillouin lasers', Optics Express, vol. 21, no. 13, pp. 16191-16191.
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Wu, WC, Yao, XZ, Jin, DY, Wang, DS, Lou, WH & Qin, XY 2013, 'Clinical strategies for differentiating autoimmune pancreatitis from pancreatic malignancy to avoid unnecessary surgical resection', Journal of Digestive Diseases, vol. 14, no. 9, pp. 500-508.
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ObjectiveThe study aimed to determine a practical strategy for differentiating between autoimmune pancreatitis (AIP) and pancreatic malignancy in order to avoid unnecessary surgical resection.MethodsAltogether, 19 patients with AIP or other pancreatic diseases underwent routine examinations including liver function test and carbohydrate antigen 19‐9, computed tomography and/or magnetic resonance imaging. Serum immunoglobulin G (IgG) and/or IgG4 was determined in patients with clinically suspected or pathologically proven AIP. Patients with suspected AIP either received diagnostic steroid therapy or laparotomy (if malignant tumors could not be excluded). Surgery was not performed in patients with a definite diagnosis of AIP by fast intraoperative frozen biopsy. Those with confirmed AIP received steroid treatment.ResultsIn total, 15 cases were finally confirmed as AIP with eight diagnosed preoperatively, five confirmed by surgical pathology (preoperatively misdiagnosed) and two by intraoperative biopsy. Of these 15 patients with AIP and one without AIP, 14 had...
Xiao, T, Hou, W, Cao, X, Wen, S, Shen, M & Shi, X 2013, 'Dendrimer-entrapped gold nanoparticles modified with folic acid for targeted gene delivery applications', Biomaterials Science, vol. 1, no. 11, pp. 1172-1172.
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Xiao, T, Wen, S, Wang, H, Liu, H, Shen, M, Zhao, J, Zhang, G & Shi, X 2013, 'Facile synthesis of acetylated dendrimer-entrapped gold nanoparticles with enhanced gold loading for CT imaging applications', Journal of Materials Chemistry B, vol. 1, no. 21, pp. 2773-2773.
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Xie, H, Liu, Y, Jin, D, Santangelo, PJ & Xi, P 2013, 'Analytical description of high-aperture STED resolution with 0–2π vortex phase modulation', Journal of the Optical Society of America A, vol. 30, no. 8, pp. 1640-1640.
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Stimulated emission depletion (STED) can achieve optical superresolution, with the optical diffraction limit broken by the suppression on the periphery of the fluorescent focal spot. Previously, it is generally experimentally accepted that there exists an inverse square root relationship with the STED power and the resolution, but with arbitrary coefficients in expression. In this paper, we have removed the arbitrary coefficients by exploring the relationship between the STED power and the achievable resolution from vector optical theory for the widely used 0-2π vortex phase modulation. Electromagnetic fields of the focal region of a high numerical aperture objective are calculated and approximated into polynomials of radius in the focal plane, and analytical expression of resolution as a function of the STED intensity has been derived. As a result, the resolution can be estimated directly from the measurement of the saturation power of the dye and the STED power applied in the region of high STED power. © 2013 Optical Society of America.
Xu, B, Jin, D, Lou, W & Wang, D 2013, 'Lipocalin‐2 is Associated With a Good Prognosis and Reversing Epithelial‐to‐Mesenchymal Transition in Pancreatic Cancer', World Journal of Surgery, vol. 37, no. 8, pp. 1892-1900.
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AbstractBackgroundLipocalin‐2 is a multifaceted modulator in cancer progression. Its clinical significance is not clear in pancreatic cancer. The purpose of this study was to investigate whether lipocalin‐2 is associated with good prognosis by reversing epithelial‐to‐mesenchymal transition (EMT) in pancreatic cancer.MethodsLipocalin‐2, E‐cadherin, or vimentin expression was detected in 60 pancreatic adenocarcinoma specimens. Correlations between lipocalin‐2 expression and EMT, the clinicopathologic characteristics, and prognosis were investigated. Whether pancreatic cancer cells’ migration and invasion (some characteristics of EMT) were affected by lipocalin‐2 was also explored.ResultsHigh lipocalin‐2 expression was significantly associated with a good prognosis in pancreatic cancer (p < 0.05). Overexpression of lipocalin‐2 correlated with a lower extent of EMT (p < 0.05), increased E‐cadherin expression (p < 0.05), decreased vimentin expression (p < 0.05), and reduced cancer cell migration and invasion in pancreatic cancer.ConclusionsLipocalin‐2 may be considered an epithelial inducer, which may reverse EMT and predict a good prognosis in pancreatic cancer.
Yakkundi, A, McCallum, L, O’Kane, A, Dyer, H, Worthington, J, McKeen, HD, McClements, L, Elliott, C, McCarthy, HO, Hirst, DG & Robson, T 2013, 'The Anti-Migratory Effects of FKBPL and Its Peptide Derivative, AD-01: Regulation of CD44 and the Cytoskeletal Pathway', PLoS ONE, vol. 8, no. 2, pp. e55075-e55075.
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FK506 binding protein-like (FKBPL) and its peptide derivatives exert potent anti-angiogenic activity in vitro and in vivo and control tumour growth in xenograft models, when administered exogenously. However, the role of endogenous FKBPL in angiogenesis is not well characterised. Here we investigated the molecular effects of the endogenous protein and its peptide derivative, AD-01, leading to their anti-migratory activity. Inhibition of secreted FKBPL using a blocking antibody or siRNA-mediated knockdown of FKBPL accelerated the migration of human microvascular endothelial cells (HMEC-1). Furthermore, MDA-MB-231 tumour cells stably overexpressing FKBPL inhibited tumour vascular development in vivo suggesting that FKBPL secreted from tumour cells could inhibit angiogenesis. Whilst FKBPL and AD-01 target CD44, the nature of this interaction is not known and here we have further interrogated this aspect. We have demonstrated that FKBPL and AD-01 bind to the CD44 receptor and inhibit tumour cell migration in a CD44 dependant manner; CD44 knockdown abrogated AD-01 binding as well as its anti-migratory activity. Interestingly, FKBPL overexpression and knockdown or treatment with AD-01, regulated CD44 expression, suggesting a co-regulatory pathway for these two proteins. Downstream of CD44, alterations in the actin cytoskeleton, indicated by intense cortical actin staining and a lack of cell spreading and communication were observed following treatment with AD-01, explaining the anti-migratory phenotype. Concomitantly, AD-01 inhibited Rac-1 activity, up-regulated RhoA and the actin binding proteins, profilin and vinculin. Thus the anti-angiogenic protein, FKBPL, and AD-01, offer a promising and alternative approach for targeting both CD44 positive tumours and vasculature networks. © 2013 Yakkundi et al.
Yang, K, Xiang, J, Bao, G, Dang, Q & Bai, X 2013, 'Synthesis of Highly Substituted 4H-Pyrido[1,2-a]pyrimidines via a One-Pot Three-Component Condensation Reaction', ACS Combinatorial Science, vol. 15, no. 9, pp. 519-524.
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Yang, Z, Liao, J, Lai, S, Wu, H, Fan, Z, Qiu, J, Song, Z, Yang, Y & Zhou, D 2013, 'Energy transfer and photoluminescence properties in Bi<SUP>3+</SUP> and Eu<SUP>3+</SUP> co-doped ZnGa<SUB>2</SUB>O<SUB>4</SUB>', Materials Express, vol. 3, no. 4, pp. 350-354.
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Yuan, X, Wen, S, Shen, M & Shi, X 2013, 'Dendrimer-stabilized silver nanoparticles enable efficient colorimetric sensing of mercury ions in aqueous solution', Analytical Methods, vol. 5, no. 20, pp. 5486-5486.
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Zhang, L, McKay, A & Jin, D 2013, 'High-throughput 3-dimensional time-resolved spectroscopy: simultaneous characterisation of luminescence properties in spectral and temporal domains', RSC Advances, vol. 3, no. 23, pp. 8670-8670.
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Lanthanide luminescence is presented in full spectral and temporal detail by challenging the limits of low-light sensing and high-speed data acquisition. A robust system is demonstrated, capable of constructing high-resolution time-resolved spectra with high throughput processing. This work holds real value in advancing characterisation capability to decode interesting insights within lanthanide materials. © The Royal Society of Chemistry 2013.
Zhao, J, Jin, D, Schartner, EP, Lu, Y, Liu, Y, Zvyagin, AV, Zhang, L, Dawes, JM, Xi, P, Piper, JA, Goldys, EM & Monro, TM 2013, 'Single-nanocrystal sensitivity achieved by enhanced upconversion luminescence', Nature Nanotechnology, vol. 8, no. 10, pp. 729-734.
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Upconversion nanocrystals convert infrared radiation to visible luminescence, and are promising for applications in biodetection1-3, bioimaging4-7, solar cells8-10 and three-dimensional display technologies8,9,11. Although the design of suitable nanocrystals has improved the performance of upconversion nanocrystals10,12-14, their emission brightness is limited by the low doping concentration of activator ions needed to avoid the luminescence quenching that occurs at high concentrations15,16. Here, we demonstrate that high excitation irradiance can alleviate concentration quenching in upconversion luminescence when combined with higher activator concentration, which can be increased from 0.5 mol% to 8 mol% Tm31 in NaYF4. This leads to significantly enhanced luminescence signals, by up to a factor of 70. By using such bright nanocrystals, we demonstrate remote tracking of a single nanocrystal with a microstructured optical-fibre dip sensor. This represents a sensitivity improvement of three orders of magnitude over benchmark nanocrystals such as quantum dots17. © 2013 Macmillan Publishers Limited. All rights reserved.
Zhao, J, Lu, Z, Yin, Y, McRae, C, Piper, JA, Dawes, JM, Jin, D & Goldys, EM 2013, 'Upconversion luminescence with tunable lifetime in NaYF4:Yb,Er nanocrystals: role of nanocrystal size', Nanoscale, vol. 5, no. 3, pp. 944-952.
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Despite recent achievements to reduce surface quenching in NaYF 4:Yb,Er nanocrystals, a complete understanding of how the nanocrystal size affects the brightness of upconversion luminescence is still incomplete. Here we investigated upconversion luminescence of Yb,Er-doped nanocrystals in a broad range of sizes from 6 nm to 45 nm (cubic or hexagonal phases), displaying an increasing red-to-green luminescence intensity ratio and reduced luminescence lifetimes with decreasing size. By analyzing the upconversion process with a set of rate equations, we found that their asymptotic analytic solutions explain lower decay rates of red compared to green upconversion luminescence. Furthermore, we quantified the effect of the surface on luminescence lifetime in a model where nanocrystal emitters are divided between the near-surface and inside regions of each nanocrystal. We clarify the influence of the four nonradiative recombination mechanisms (intrinsic phonon modes, vibration energy of surface ligands, solvent-mediated quenching, and surface defects) on the decay rates for different-size nanocrystals, and find that the defect density dominates decay rates for small (below 15 nm) nanocrystals. Our results indicate that a defect-reduction strategy is a key step in producing small upconversion nanocrystals with increased brightness for a variety of bioimaging and biosensing applications. © 2013 The Royal Society of Chemistry.
Zheng, F, Wang, S, Wen, S, Shen, M, Zhu, M & Shi, X 2013, 'Characterization and antibacterial activity of amoxicillin-loaded electrospun nano-hydroxyapatite/poly(lactic-co-glycolic acid) composite nanofibers', Biomaterials, vol. 34, no. 4, pp. 1402-1412.
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Zhou, J, Deng, J, Zhu, H, Chen, X, Teng, Y, Jia, H, Xu, S & Qiu, J 2013, 'Up-conversion luminescence in LaF3:Ho3+via two-wavelength excitation for use in solar cells', Journal of Materials Chemistry C, vol. 1, no. 48, pp. 8023-8023.
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An efficient broadband excited near-infrared to visible up-conversion is observed in LaF3:Ho3+ as the result of a two-wavelength excitation. The visible up-conversion emission intensity is greatly enhanced upon simultaneous excitation at 970 nm and 1150 nm, due to an energy transfer up-conversion mechanism. Multi-wavelength excitation based on the ground-state absorption, excited-state absorption, and phonon-coupled absorption of rare-earth ions results in an efficient broadband excited up-conversion emission, which may provide a new approach to fully harvest NIR solar energy and has potential application in solar cells.
Zhou, J, Shirahata, N, Sun, H-T, Ghosh, B, Ogawara, M, Teng, Y, Zhou, S, Chu, RGS, Fujii, M & Qiu, J 2013, 'Efficient Dual-Modal NIR-to-NIR Emission of Rare Earth Ions Co-doped Nanocrystals for Biological Fluorescence Imaging', JOURNAL OF PHYSICAL CHEMISTRY LETTERS, vol. 4, no. 3, pp. 402-408.
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A novel approach has been developed for the realization of efficient near-infrared to near-infrared (NIR-to-NIR) upconversion and down-shifting emission in nanophosphors. The efficient dual-modal NIR-to-NIR emission is realized in a β-NaGdF4/Nd3+@NaGdF4/Tm3+–Yb3+ core–shell nanocrystal by careful control of the identity and concentration of the doped rare earth (RE) ion species and by manipulation of the spatial distributions of these RE ions. The photoluminescence results reveal that the emission efficiency increases at least 2-fold when comparing the materials synthesized in this study with those synthesized through traditional approaches. Hence, these core–shell structured nanocrystals with novel excitation and emission behaviors enable us to obtain tissue fluorescence imaging by detecting the upconverted and down-shifted photoluminescence from Tm3+ and Nd3+ ions, respectively. The reported approach thus provides a new route for the realization of high-yield emission from RE ion doped nanocrystals, which could prove to be useful for the design of optical materials containing other optically active centers.
Zhu, Y, Gupta, B, Guan, B, Ciampi, S, Reece, PJ & Gooding, JJ 2013, 'Photolithographic Strategy for Patterning Preformed, Chemically Modified, Porous Silicon Photonic Crystal Using Click Chemistry', ACS Applied Materials & Interfaces, vol. 5, no. 14, pp. 6514-6521.
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Zou, LE, Kabakova, IV, Mägi, EC, Li, E, Florea, C, Aggarwal, ID, Shaw, B, Sanghera, JS & Eggleton, BJ 2013, 'Efficient inscription of Bragg gratings in As_2S_3 fibers using near bandgap light', Optics Letters, vol. 38, no. 19, pp. 3850-3850.
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Buttner, TFS, Kabakova, IV, Hudson, DD & Eggleton, BJ 1970, 'Picosecond pulse burst generation using cascaded Stimulated Brillouin Scattering in a chalcogenide As<inf>2</inf>Se<inf>3</inf> fiber cavity', 2013 Conference on Lasers & Electro-Optics Europe & International Quantum Electronics Conference CLEO EUROPE/IQEC, 2013 Conference on Lasers & Electro-Optics Europe & International Quantum Electronics Conference CLEO EUROPE/IQEC, IEEE.
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Stimulated Brillouin Scattering (SBS) is an inelastic scattering process that has been exploited for applications ranging from tunable delay, microwave generation to sensing and lasers. The recent demonstration [1] of a frequency comb laser based on cascaded SBS in silica fiber has renewed interest in using SBS to generate ultra-high frequency picosecond pulses [2]. In [1] a broad SBS frequency comb is generated in a long (e.g. kilometres) ring cavity using an erbium doped fiber amplifier (EDFA) as gain medium. © 2013 IEEE.
Büttner, TFS, Kabakova, IV, Hudson, DD & Eggleton, BJ 1970, 'Picosecond pulse burst generation using cascaded Stimulated Brillouin Scattering in a chalcogenide As2Se3 fiber cavity', Optics InfoBase Conference Papers.
Cranfield, CG, Cornell, B, Grage, SL, Duckworth, P, Carne, S, Ulrich, AS & Martinac, B 1970, 'Characterization of Antimicrobial Peptide Insertion in Tethered Bilayer Lipid Membranes by Pulse Amperometry and Linear Sweep Voltammetry Methods', BIOPHYSICAL JOURNAL, 57th Annual Meeting of the Biophysical-Society, CELL PRESS, Philadelphia, PA, pp. 600A-600A.
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Dawes, JM, Zhao, J, Lu, Z, Yin, Y, Zhang, L, McKay, A, McRae, C, Piper, J, Jin, D & Goldys, EM 1970, 'Characterisation of Upconversion Nanoparticles for Imaging', Asia Communications and Photonics Conference 2013, Asia Communications and Photonics Conference, OSA.
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Eggleton, BJ, Poulton, CG, Marpaung, D, Morrison, B, Kabakova, IV, Choi, D-Y, Madden, SJ, Luther-Davies, B & Pant, R 1970, 'On-chip stimulated Brillouin scattering and its applications', SPIE Proceedings, SPIE NanoScience + Engineering, SPIE, San Diego, California, United States.
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We review recent demonstration of stimulated Brillouin scattering in a chalcogenide photonic chip and its application to optical and microwave signal processing tasks. The interaction between light and sound via stimulated Brillouin scattering (SBS) was exploited in chalcogenide photonic circuits to achieve on-chip SBS slow and fast light, microwave photonic filters, and dynamic gratings using travelling-wave geometry. Using a ring-resonator geometry, photonic-chip based Brillouin laser was demonstrated. © 2013 SPIE.
Eilenberger, F, Kabakova, I, Martin De Sterke, C, Eggleton, BJ & Pertsch, T 1970, 'Cavity optical pulse extraction: A novel, ultrashort light source based on nonlinearly seeded hawking radiation', Optics InfoBase Conference Papers.
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We show that light trapped in a linear cavity can be efficiently released into an ultrashort coherent pulse, using a non-resonant trigger pulse. We derive a simplified analytic description of the process and confirm its complex behavior by numerical simulation. This 'Cavity Optical Pulse Extraction' (COPE) process is shown to be analogous to classical Hawking radiation emitted from an optical event horizon with extremely high Hawking temperature. Nonlinear Optics Technical Digest. © OSA 2013.
Eilenberger, F, Kabakova, I, Martin de Sterke, C, Eggleton, BJ & Pertsch, T 1970, 'Cavity Optical Pulse Extraction: a novel, ultrashort light source based on nonlinearly seeded Hawking radiation', Nonlinear Optics, Nonlinear Optics, OSA.
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Guan, G, Warkiani, ME, Luan, KB, Lim, CT, Chen, PCY & Han, J 1970, 'High throughput circulating tumor cell isolation using trapezoidal inertial microfluidics', 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, pp. 23-25.
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A novel Dean coupled inertial microfluidic device with trapezoidal cross-section spiral channel for ultra-fast, label- free enrichment of circulating tumor cells (CTCs) from clinically relevant blood volumes is reported in this work. Using this single spiral microchannel with one inlet and two outlets, we have successfully isolated and recovered more than 80% of cancer cell line cells spiked in 7.5 mL of blood within 8 min with high purity. Putative CTCs were detected and isolated from 100% patient samples with advanced stage metastatic breast and lung cancer using standard biomarkers. DNA fluorescence in-situ hybridization (FISH) was also carried out to evaluate HER2 status in CTCs isolated from patient samples. Copyright © (2013) by the Chemical and Biological Microsystems Society All rights reserved.
Jing, T, Ramji, R, Warkiani, ME, Lim, CT, Han, J & Chen, CH 1970, 'High throughput single cancer cell encapsulation and self sorting for protease assay by using jetting microfluidics', 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, pp. 1373-1375.
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In this work, a droplet microfluidic device integrated with droplet sorting function was presented for single cancer cell encapsulations and protease activity measurements. Individual cells were encapsulated into aqueous droplets at flow focusing junction under jetting conditions. Droplets with cells encapsulated inside are larger than other empty droplets, enabling effective droplet sorting through a deterministic lateral displacement micro-pillar design. All droplets with cells are therefore collected by an observation chamber for enzymatic activity monitoring. Here we focused on measuring matrix metalloproteinase (MMPs) secretion intensities at the individual cell level to obtain further insights into the malignant characteristics of these cells.
Kabakova, IV, Büttner, TFS & Eggleton, BJ 1970, 'Frequency Combs Formed via Stimulated Brillouin Scattering in Chalcogenide', Workshop on Specialty Optical Fibers and their Applications, Workshop on Specialty Optical Fibers and their Applications, OSA.
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Kabakova, IV, Büttner, TFS & Eggleton, BJ 1970, 'Frequency combs formed via stimulated brillouin scattering in chalcogenide', Optics InfoBase Conference Papers.
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We exploit the strong Brillouin gain of chalcogenide glasses to generate frequency combs in a centimeter scale fiber devices. These can be applied to generation of ultra-high frequency picosecond pulse trains or complex reflection filters. © OSA 2013.
Kabakova, IV, Pant, R, Luther-Davies, B, Choi, D-Y, Madden, SJ, Eggleton, BJ & Debbarma, S 1970, 'Narrow linewidth Brillouin laser based on chalcogenide chip', 39th European Conference and Exhibition on Optical Communication (ECOC 2013), 39th European Conference and Exhibition on Optical Communication (ECOC 2013), Institution of Engineering and Technology, pp. 411-413.
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We demonstrate a narrow linewidth Brillouin ring-cavity laser based on chalcogenide photonic chip. The gain medium is a 7 cm-long As2S 3 waveguide with a cross-section of 4μm x 850nm and the Brillouin gain coefficient of 0.7 10-9 m/W. The waveguides are equipped with vertical tapers so that the coupling loss is reduced. The lasing threshold is approximately PL=360 mW, almost 5 times lower than the calculated single-pass threshold P0=1.73 W in the same waveguide. The slope efficiency is found to be 30% and the linewidth of 100 kHz is measured using a self-heterodyne method.
Lu, Y, Lu, J, Piper, JA, Xi, P & Jin, D 1970, 'Orthogonal scanning automated microscopy (OSAM) speeds up time-gated luminescence detection', Bio-Optics: Design and Application, BODA 2013.
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We report a versatile platform of orthogonal scanning automated microscopy (OSAM) cooperated with time-gated detection technique. Featuring rapid processing and background-free detection, it offers ultra-high sensitivity to spot rare-event cells and quantitate low-expression surface molecules. Optics in the Life Sciences Congress Technical Digest © 2013 The Optical Society (OSA).
Lu, Y, Lu, J, Piper, JA, Xi, P & Jin, D 1970, 'Orthogonal scanning automated microscopy speeds up time-gated luminescence detection', Optical Trapping Applications, OTA 2013.
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We report a versatile platform of orthogonal scanning automated microscopy (OSAM) cooperated with time-gated detection technique. Featuring rapid processing and background-free detection, it offers ultra-high sensitivity to spot rare-event cells and quantitate low-expression surface molecules. © 2013 The Optical Society (OSA).
Lu, Y, Lu, J, Piper, JA, Xi, P & Jin, D 1970, 'Orthogonal Scanning Automated Microscopy Speeds Up Time-Gated Luminescence Detection', Optics in the Life Sciences, Bio-Optics: Design and Application, OSA.
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We report a versatile platform of orthogonal scanning automated microscopy (OSAM) cooperated with time-gated detection technique. Featuring rapid processing and background-free detection, it offers ultra-high sensitivity to spot rare-event cells and quantitate low-expression surface molecules. ©2013 The Optical Society (OSA).
Nickel, F, Gottlob, DM, Krug, IP, Doganay, H, Cramm, S, Kaiser, AM, Lin, G, Makarov, D, Schmidt, OG & Schneider, CM 1970, 'Time-resolved magnetic imaging in an aberration-corrected, energy-filtered photoemission electron microscope', Ultramicroscopy, 8th International Workshop on Low Energy Electron Microscopy and Photoemission Electron Microscopy (LEEM/PEEM), Elsevier BV, Hong Kong, PEOPLES R CHINA, pp. 54-62.
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Schartner, EP, Jin, D, Zhao, J & Monro, TM 1970, 'Sensitive detection of NaYF4: Yb/Tm nanoparticles using suspended core microstructured optical fibers', Colloidal Nanocrystals for Biomedical Applications VIII, SPIE BiOS, SPIE, San Francisco, California, United States.
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Rare-earth doped upconversion nanocrystals are emerging as the next-generation luminescent biomaterials. Here we load NaYF4: Yb/Er and NaYF4: Yb/Tm upconversion nanocrystals into a soft-glass suspended-core optical fiber dip sensor, allowing sensitive measurements and power-dependent characterizations to be performed. This, in combination with negligible background autofluorescence from the glass fiber when using infrared excitation has provided a significant improvement in terms of sensitivity over what has previously been demonstrated using an optical fiber dip sensor. For detection we employ suspended-core optical fibers, which have found extensive use in sensing applications. These combine the high evanescent overlap comparable to that of a nanowire, with the robust handling characteristics and long interaction length of a conventional fiber. The fiber sensor platform allows measurements to be performed using minimal sample volumes (<20 nL) while still maintaining the sensitivity of the platform. © 2013 Copyright SPIE.
Warkiani, ME, Khoo, BL, Tan, DSW, Bhagat, AAS, Lim, WT, Han, J & Lim, CT 1970, 'Circulating tumor cell (CTC) enrichment: Ultra high throughput processing of clinically relevant blood volumes using a multiplexed spiral biochip', 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, pp. 1156-1158.
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Detection and characterization of circulating tumor cells (CTCs) from minimally invasive 'liquid biopsy' provides critical insights into tumor biology and is critical for companion diagnostics and care. Here, we present a multiplexed spiral biochip for ultra-high throughput isolation of CTCs using inertial microfluidics to realize a single step label-free enrichment process. This device is capable of efficient cell separation of clinically relevant blood volumes in a short period of time (7.5 mL blood in 35 min). CTCs were successfully detected and isolated from 100% (50/50) blood samples collected from patients with advanced stage metastatic breast and lung cancer. They were identified under immunofluorescence assays (cytokeratin positive), as well as molecular probes (EGFR or HER2 positive). CTC recovery rate ranges from 3-1535 CTCs/mL and obtained under high purity (1 CTC for every 30-100 white blood cells detected). Retrieved cells are unlabelled and hence more viable for propagation, drug development and other downstream analysis.
Watts, AL, Singh, N, Poulton, CG, Magi, E, Kabakova, IV, Hudson, D & Eggleton, BJ 1970, 'Characterization of axial quantization in WGM chalcogenide microfiber resonators', Frontiers in Optics 2013, Frontiers in Optics, OSA.
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We experimentally and numerically characterize the axial quantization in whispering gallery mode (WGM) microfiber resonators exploiting a positive photoinduced index perturbation. Experimental results are in good agreement with numerical calculations. © OSA 2013.
Watts, AL, Singh, N, Poulton, CG, Magi, E, Kabakova, IV, Hudson, D & Eggleton, BJ 1970, 'Characterization of axial quantization in WGM chalcogenide microfiber resonators', Optics InfoBase Conference Papers.
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We experimentally and numerically characterize the axial quantization in whispering gallery mode (WGM) microfiber resonators exploiting a positive photoinduced index perturbation. Experimental results are in good agreement with numerical calculations. © OSA 2013.
Wen, S, Liu, H, Cai, H, Shen, M & Shi, X 1970, 'Targeted and pH-responsive delivery of doxorubicin to cancer cells using mutifunctional dendrimer-modified multi-walled carbon nanotubes', ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 245th National Spring Meeting of the American-Chemical-Society (ACS), AMER CHEMICAL SOC, New Orleans, LA.
Xi, P, Xie, H, Liu, Y & Jin, D 1970, 'Stimulated emission depletion point spread function generation with vector solution', Nanophotonics, Nanoelectronics and Nanosensor, N3 2013.
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The excitation and depletion point spread functions of stimulated emission depletion optical microscopy have been derived, based on the vectorial diffraction theory. The performance of STED with high numerical aperture objective can be simulated with this method. © OSA 2013.
Xie, H, Ding, Y, Liu, Y, Zhang, L, Jin, D, Santangelo, PJ, Ren, Q & Xi, P 1970, 'STED3D: point spread function simulation for high numerical aperture objective and resolution evaluation', Optics in the Life Sciences, Bio-Optics: Design and Application, OSA.
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We developed STED3D to accelerate the calculation of point spread function with vector diffraction theory. Electromagnetic fields for both excitation and vortex modulated depletion beams can be simulated, to yield the resolution of STED nanoscopy.©Optics in the Life Sciences Congress Technical Digest.
Xie, H, Ding, Y, Liu, Y, Zhang, L, Jin, D, Santangelo, PJ, Ren, Q & Xi, P 1970, 'STED3D: Point spread function simulation for high numerical aperture objective and resolution evaluation', Bio-Optics: Design and Application, BODA 2013.
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We developed STED3D to accelerate the calculation of point spread function with vector diffraction theory. Electromagnetic fields for both excitation and vortex modulated depletion beams can be simulated, to yield the resolution of STED nanoscopy. Optics in the Life Sciences Congress Technical Digest © 2013 The Optical Society (OSA).
Xie, H, Ding, Y, Liu, Y, Zhang, L, Jin, D, Santangelo, PJ, Ren, Q & Xi, P 1970, 'Sted3D: Point spread function simulation for high numerical aperture objective and resolution evaluation', Optical Trapping Applications, OTA 2013.
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We developed STED3D to accelerate the calculation of point spread function with vector diffraction theory. Electromagnetic fields for both excitation and vortex modulated depletion beams can be simulated, to yield the resolution of STED nanoscopy. © 2013 The Optical Society (OSA).
Yu, Z, Kabakova, IV, Fonjallaz, PY, Tarasenko, O, Margulis, W & De Sterke, CM 1970, 'Dynamic frequency tuning in a fiber grating cavity', Bragg Gratings, Photosensitivity, and Poling in Glass Waveguides, BGPP 2012.
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Dynamic frequency tuning of trapped light in a phase-shifted fiber grating cavity is demonstrated by high-voltage electrical pulses. Y-polarization light is found to be sensitive to refractive index changes caused by a transverse pressure-wave. © 2012 OCA.
Yu, Z, Rugeland, P, Kabakova, IV, Fonjallaz, PY, Tarasenko, O, De Sterke, CM & Margulis, W 1970, 'Dynamic wavelength switching in a fiber grating cavity', CLEO: Science and Innovations, CLEO_SI 2013.
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Dynamic wavelength switching of a phase-shifted fiber grating cavity with electrical pulses is studied experimentally and numerically. Simulations accurately describe observations. The effect of acoustic oscillations and fiber Bragg grating cavity refilling explains the results. © 2013 Optical Society of America.
Yu, Z, Rugeland, P, Kabakova, IV, Fonjallaz, P-Y, Tarasenko, O, de Sterke, CM & Margulis, W 1970, 'Dynamic Wavelength Switching in a Fiber Grating Cavity', CLEO: 2013, CLEO: Science and Innovations, OSA, San Jose, CA.
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Zhao, J, Lu, Z, Yin, Y, Zhang, L, McKay, A, McRae, C, Piper, JA, Jin, D, Goldys, EM & Dawes, JM 1970, 'Characterisation of Upconversion Nanoparticles for Imaging', Asia Communications and Photonics Conference 2013, Asia Communications and Photonics Conference, OSA, Beijing, China.
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By controlling the size of Yb,Er, doped upconversion nanoparticles, we obtain varying emission lifetimes and emission ratios of green and red light, which we explain by modelling of excited state decay mechanisms. © OSA 2013.
Zheng, F, Wang, S, Wen, S, Shen, M & Shi, X 1970, 'Amoxicillin-loaded electrospun nano-hydroxyapatite/poly(lactic-co-glycolic acid) composite nanofibers: Preparation, characterization and antibacterial activity', Journal of Controlled Release, Elsevier BV, pp. e30-e31.
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