Buettner, TFS, Kabakova, IV, Hudson, DD, Pant, R, Poulton, CG, Judge, AC & Eggleton, BJ 2014, 'Phase-locking in Multi-Frequency Brillouin Oscillator via Four Wave Mixing', Scientific Reports, vol. 4.
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Stimulated Brillouin scattering (SBS) and Kerr-nonlinear four wave-mixing(FWM) are among the most important and widely studied nonlinear effects inoptical fibres. At high powers SBS can be cascaded producing multiple Stokeswaves spaced by the Brillouin frequency shift. Here, we investigate the complexnonlinear interaction of the cascade of Stokes waves, generated in aFabry-Perot chalcogenide fibre resonator through the combined action of SBS andFWM. We demonstrate the existence of parameter regimes, in which pump andStokes waves attain a phase-locked steady state. Real-time measurements of 40pspulses with 8GHz repetition rate are presented, confirming short-and long-termstability. Numerical simulations qualitatively agree with experiments and showthe significance of FWM in phase-locking of pump and Stokes waves. Our findingscan be applied for the design of novel picosecond pulse sources with GHzrepetition rate for optical communication systems.
Büttner, TFS, Merklein, M, Kabakova, IV, Hudson, DD, Choi, D-Y, Luther-Davies, B, Madden, SJ & Eggleton, BJ 2014, 'Phase-locked, chip-based, cascaded stimulated Brillouin scattering', Optica, vol. 1, no. 5, pp. 311-311.
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Cheng, G, Wu, H, Yang, Z, Liao, J, Lai, S, Qiu, J & Song, Z 2014, 'Upconversion emission properties of CeO2: Tm3+, Yb3+ inverse opal photonic crystals', Modern Physics Letters B, vol. 28, no. 27, pp. 1450218-1450218.
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The ordered and disordered templates were assembled by vertical deposition of polystyrene microspheres. The CeO 2: Tm 3+, Yb 3+ precursor solution was used to infiltrate into the voids of the ordered and disordered templates, respectively. Then the ordered and disordered templates were calcined at 950°C in an air furnace, and the CeO 2: Tm 3+, Yb 3+ inverse opals were obtained. The upconversion emissions from CeO 2: Tm 3+, Yb 3+ inverse opals were suppressed due to the photon trapping caused by Bragg reflection of lattice planes when the upconversion emission band was in the range of the photonic band gaps in the inverse opals.
Cranfield, CG, Cornell, BA, Grage, SL, Duckworth, P, Carne, S, Ulrich, AS & Martinac, B 2014, 'Transient Potential Gradients and Impedance Measures of Tethered Bilayer Lipid Membranes: Pore-Forming Peptide Insertion and the Effect of Electroporation', BIOPHYSICAL JOURNAL, vol. 106, no. 1, pp. 182-189.
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In this work, we present experimental data, supported by a quantitative model, on the generation and effect of potential gradients across a tethered bilayer lipid membrane (tBLM) with, to the best of our knowledge, novel architecture. A challenge to generating potential gradients across tBLMs arises from the tethering coordination chemistry requiring an inert metal such as gold, resulting in any externally applied voltage source being capacitively coupled to the tBLM. This in turn causes any potential across the tBLM assembly to decay to zero in milliseconds to seconds, depending on the level of membrane conductance. Transient voltages applied to tBLMs by pulsed or ramped direct-current amperometry can, however, provide current-voltage (I/V) data that may be used to measure the voltage dependency of the membrane conductance. We show that potential gradients >~150 mV induce membrane defects that permit the insertion of pore-forming peptides. Further, we report here the novel (to our knowledge) use of real-time modeling of conventional low-voltage alternating-current impedance spectroscopy to identify whether the conduction arising from the insertion of a polypeptide is uniform or heterogeneous on scales of nanometers to micrometers across the membrane. The utility of this tBLM architecture and these techniques is demonstrated by characterizing the resulting conduction properties of the antimicrobial peptide PGLa.
Cun, Y, Yang, Z, Liao, J, Qiu, J, Song, Z & Yang, Y 2014, 'Enhancement of upconversion luminescence of three-dimensional ordered macroporous Bi2Ti2O7:Er3+, Yb3+ by co-doping of Li+ ions', Materials Letters, vol. 131, pp. 154-157.
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Donley, C, McClelland, K, McKeen, HD, Nelson, L, Yakkundi, A, Jithesh, PV, Burrows, J, McClements, L, Valentine, A, Prise, KM, McCarthy, HO & Robson, T 2014, 'Identification of RBCK1 as a novel regulator of FKBPL: implications for tumor growth and response to tamoxifen', Oncogene, vol. 33, no. 26, pp. 3441-3450.
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Fu, F, Wu, Y, Zhu, J, Wen, S, Shen, M & Shi, X 2014, 'Multifunctional Lactobionic Acid-Modified Dendrimers for Targeted Drug Delivery to Liver Cancer Cells: Investigating the Role Played by PEG Spacer', ACS Applied Materials & Interfaces, vol. 6, no. 18, pp. 16416-16425.
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Han, X, Xu, X, Jin, D, Wang, D, Ji, Y & Lou, W 2014, 'Clinicopathological Characteristics and Prognosis-Related Factors of Resectable Pancreatic Neuroendocrine Tumors', Pancreas, vol. 43, no. 4, pp. 526-531.
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Hoiles, W, Krishnamurthy, V, Cranfield, CG & Cornell, B 2014, 'An Engineered Membrane to Measure Electroporation: Effect of Tethers and Bioelectronic Interface', BIOPHYSICAL JOURNAL, vol. 107, no. 6, pp. 1339-1351.
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© 2014 by the Biophysical Society. This article reports on the construction and predictive models for a platform comprised of an engineered tethered membrane. The platform provides a controllable and physiologically relevant environment for the study of the electroporation process. The mixed self-assembled membrane is formed via a rapid solvent exchange technique. The membrane is tethered to the gold electrode and includes an ionic reservoir separating the membrane and gold surface. Above the membrane, there is an electrolyte solution, and a gold counterelectrode. A voltage is applied between the gold electrodes and the current measured. The current is dependent on the energy required to form aqueous pores and the conductance of each pore. A two-level predictive model, consisting of a macroscopic and a continuum model, is developed to relate the pore dynamics to the measured current. The macroscopic model consists of an equivalent circuit model of the tethered membrane, and asymptotic approximations to the Smoluchowski-Einstein equation of electroporation that is dependent on the pore conductance and the energy required to form aqueous pores. The continuum model is a generalized Poisson-Nernst-Planck (GPNP) system where an activity coefficient to account for steric effects of ions is added to the standard PNP system. The GPNP is used to evaluate the conductance of aqueous pores, and the electrical energy required to form the pores. As an outcome of the setup of the device and the two-level model, biologically important variables can be estimated from experimental measurements. To validate the accuracy of the two-level model, the predicted current is compared with experimentally measured current for different tethering densities.
Hu, K, Kabakova, IV, Büttner, TFS, Lefrancois, S, Hudson, DD, He, S & Eggleton, BJ 2014, 'Low-threshold Brillouin laser at 2 μm based on suspended-core chalcogenide fiber', Optics Letters, vol. 39, no. 16, pp. 4651-4651.
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We present, to the best of our knowledge, the first demonstration of a 2 μm Brillouin laser based on a thulium-doped fiber pump and a chalcogenide fiber. A short 1.5mpiece of suspended-core chalcogenide As38Se 62 fiber is employed as a gain medium, taking advantage of its small effective mode area and high Brillouin gain coefficient. A record-low lasing threshold of 52 mW is achieved, which is about 10 times lower than previously demonstrated in silica fiber cavities. © 2014 Optical Society of America.
Hu, K, Kabakova, IV, Lefrancois, S, Hudson, DD, He, S & Eggleton, BJ 2014, 'Hybrid Brillouin/thulium multiwavelength fiber laser with switchable single- and double-Brillouin-frequency spacing', Optics Express, vol. 22, no. 26, pp. 31884-31884.
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Huang, Y, Hu, D, Wen, S, Shen, M, Zhu, M & Shi, X 2014, 'Selective removal of mercury ions using thymine-grafted electrospun polymer nanofibers', NEW JOURNAL OF CHEMISTRY, vol. 38, no. 4, pp. 1533-1539.
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Jiang, L, Phang, JM, Yu, J, Harrop, SJ, Sokolova, AV, Duff, AP, Wilk, KE, Alkhamici, H, Breit, SN, Valenzuela, SM, Brown, LJ & Curmi, PMG 2014, 'CLIC proteins, ezrin, radixin, moesin and the coupling of membranes to the actin cytoskeleton: A smoking gun?', BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, vol. 1838, no. 2, pp. 643-657.
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The CLIC proteins are a highly conserved family of metazoan proteins with the unusual ability to adopt both soluble and integral membrane forms. The physiological functions of CLIC proteins may include enzymatic activity in the soluble form and anion channel activity in the integral membrane form. CLIC proteins are associated with the ERM proteins: ezrin, radixin and moesin. ERM proteins act as cross-linkers between membranes and the cortical actin cytoskeleton. Both CLIC and ERM proteins are controlled by Rho family small GTPases. CLIC proteins, ERM and Rho GTPases act in a concerted manner to control active membrane processes including the maintenance of microvillar structures, phagocytosis and vesicle trafficking. All of these processes involve the interaction of membranes with the underlying cortical actin cytoskeleton. The relationships between Rho GTPases, CLIC proteins, ERM proteins and the membrane:actin cytoskeleton interface are reviewed. Speculative models are proposed involving the formation of localised multi-protein complexes on the membrane surface that assemble via multiple weak interactions. This article is part of a Special Issue entitled: Reciprocal influences between cell cytoskeleton and membrane channels, receptors and transporters
Kabakova, IV, Marpaung, D, Poulton, CG & Eggleton, BJ 2014, 'Driving acoustic waves optically on a chip', Australian Physics, vol. 51, no. 3, pp. 84-88.
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Stimulated Brillouin scattering (SBS) is an important nonlinear effect in optical fibers and waveguides that has been traditionally exploited for high-sensitivity distributed sensing, coherent lasers and gyroscopes. Recent advances in nanofabrication led to the extensive growth of SBS research. The ability to generate SBS in nanoscale devices has opened numerous opportunities for photonic integration, resulting in on-chip Brillouin lasers, microwave generation, Brillouin cooling and quantum optomechanics. In this paper we briefly describe principles of inelastic Brillouin scattering and review our recent developments in optical signal manipulation and lasing using on-chip SBS.
Kabakova, IV, Pant, R, Winful, HG & Eggleton, BJ 2014, 'Chalcogenide Brillouin lasers', Journal of Nonlinear Optical Physics & Materials, vol. 23, no. 01, pp. 1450001-1450001.
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In recent years, chalcogenide glasses have emerged as a material platform for nonlinear signal processing and mid-infrared photonics. In addition, high Brillouin gain of these soft glasses enable compact and highly efficient Brillouin lasers (BL). We review recent progress on chalcogenide BL, compare fiber and waveguide laser geometries, and discuss the potential of chalcogenide platform for compact and high-coherence light sources.
Khoo, BL, Warkiani, ME, Tan, DSW, Bhagat, AAS & Irwin, D 2014, 'Erratum: Clinical validation of an ultra high-throughput spiral microfluidics for the detection and enrichment of viable circulating tumor cells (PLoS ONE 9(7) e99409). doi:10.1371/journal.pone.0099409', PLoS ONE, vol. 9, no. 10.
Khoo, BL, Warkiani, ME, Tan, DS-W, Bhagat, AAS, Irwin, D, Lau, DP, Lim, AST, Lim, KH, Krisna, SS, Lim, W-T, Yap, YS, Lee, SC, Soo, RA, Han, J & Lim, CT 2014, 'Clinical Validation of an Ultra High-Throughput Spiral Microfluidics for the Detection and Enrichment of Viable Circulating Tumor Cells', PLoS ONE, vol. 9, no. 7, pp. e99409-e99409.
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BACKGROUND: Circulating tumor cells (CTCs) are cancer cells that can be isolated via liquid biopsy from blood and can be phenotypically and genetically characterized to provide critical information for guiding cancer treatment. Current analysis of CTCs is hindered by the throughput, selectivity and specificity of devices or assays used in CTC detection and isolation. METHODOLOGY/PRINCIPAL FINDINGS: Here, we enriched and characterized putative CTCs from blood samples of patients with both advanced stage metastatic breast and lung cancers using a novel multiplexed spiral microfluidic chip. This system detected putative CTCs under high sensitivity (100%, n = 56) (Breast cancer samples: 12-1275 CTCs/ml; Lung cancer samples: 10-1535 CTCs/ml) rapidly from clinically relevant blood volumes (7.5 ml under 5 min). Blood samples were completely separated into plasma, CTCs and PBMCs components and each fraction were characterized with immunophenotyping (Pan-cytokeratin/CD45, CD44/CD24, EpCAM), fluorescence in-situ hybridization (FISH) (EML4-ALK) or targeted somatic mutation analysis. We used an ultra-sensitive mass spectrometry based system to highlight the presence of an EGFR-activating mutation in both isolated CTCs and plasma cell-free DNA (cf-DNA), and demonstrate concordance with the original tumor-biopsy samples. CONCLUSIONS/SIGNIFICANCE: We have clinically validated our multiplexed microfluidic chip for the ultra high-throughput, low-cost and label-free enrichment of CTCs. Retrieved cells were unlabeled and viable, enabling potential propagation and real-time downstream analysis using next generation sequencing (NGS) or proteomic analysis.
Lai, S, Yang, Z, Liao, J, Li, J, Shao, B, Qiu, J & Song, Z 2014, 'Preparation and photoluminescence property of SiO 2 :Tb 3+ three dimensionally ordered macroporous composites including Ag nanoparticles', Journal of Non-Crystalline Solids, vol. 405, pp. 176-179.
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Lai, S, Yang, Z, Liao, J, Qiu, J, Song, Z, Yang, Y & Zhou, D 2014, 'Investigation of persistent luminescence property of Bi 3+, Dy 3+ co-doped CdSiO 3 phosphor', Materials Research Bulletin, vol. 60, pp. 714-718.
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Li, K, Wang, S, Wen, S, Tang, Y, Li, J, Shi, X & Zhao, Q 2014, 'Enhanced In Vivo Antitumor Efficacy of Doxorubicin Encapsulated within Laponite Nanodisks', ACS Applied Materials & Interfaces, vol. 6, no. 15, pp. 12328-12334.
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Li, RQ, Qiu, JB, Yang, ZW, Liao, JY, Wu, HJ, Lai, SF, Song, ZG, Yang, Y, Zhou, DC & Wang, RF 2014, 'Preparation and up-conversion luminescence properties of Yb3+/Tm3+ Co-doped Sb2O4 powder', Guang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy and Spectral Analysis, vol. 34, no. 3, pp. 630-633.
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The Sb2O4:Yb3+, Tm3+ up-conversion luminescence powder with excellent physical, chemical stability and relative low phonon energy was synthesized by the high temperature solid-state reaction and its up-conversion luminescence property was investigated. Under the 980 nm excitation, infrared and blue up-conversion emissions centered at 800 and 480 nm were observed, which were assigned to the 1G4→3H6 and 3H4→3H6 transitions of Tm3+, respectively. The influence of Yb3+ and Tm3+ concentration on the up-conversion emission property was also obtained. The up-conversion luminescence increases with increasing of Yb3+ and Tm3+ concentration. Additionally, the up-conversion luminescence mechanism was discussed based on the dependence of Tm3+ up-conversion luminescence on pump power. It is interesting that two photon excitation processes for blue and infrared emission were observed in the Sb2O4:Yb3+, Tm3+ powder under a 980 nm excitation. Based on the energy level diagram of Tm3+ and Yb3+ ions, we think that two photons blue emission is contributed to the cooperation energy transfer between Tm3+ and Yb3+ ions. We believe that the Sb2O4:Yb3+, Tm3+ up-conversion luminescence powder will have potential application for new optical devices in up-conversion color displays, sensors, detection of infrared radiation, and lasers.
Liao, J, Yang, Z, Lai, S, Shao, B, Li, J, Qiu, J, Song, Z & Yang, Y 2014, 'Upconversion Emission Enhancement of NaYF4:Yb,Er Nanoparticles by Coupling Silver Nanoparticle Plasmons and Photonic Crystal Effects', The Journal of Physical Chemistry C, vol. 118, no. 31, pp. 17992-17999.
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Metal nanoparticle plasmons or the photonic crystal effect are being widely used to modify luminescence properties of materials. However, coupling of surface plasmons with photonic crystals are seldom reported for enhancing luminescence of materials. In this paper, a new method for upconversion emission enhancement of rare-earth doped nanoparticles is reported, attributed to the coupling of surface plasmons with photonic band gap effects. Opal/Ag hybrid substrates were prepared by depositing Ag nanoparticles on the top layer of opals by magnetron sputtering. The selective enhancement of red or green upconversion emission of NaYF4:Yb3+,Er3+ nanoparticles on the opal/Ag hybrid substrates is attributed to the coupling effect of surface plasmons and Bragg reflection of the photonic band gap. In addition, the upconversion emission enhancement of NaYF4:Yb 3+,Er3+ nanoparticles on the opal/Ag hybrid substrate is attributed to the excitation enhancement was obtained when the excitation light wavelengths overlap with the photonic band gaps of opal/Ag hybrid substrates. We believe that these enhancement effects based on the coupling of metal nanoparticles with the photonic band gap could be extended to other light-emitting materials, which may result in a new generation of lighting devices. © 2014 American Chemical Society.
Liao, J, Yang, Z, Wu, H, Lai, S, Qiu, J, Song, Z, Yang, Y & Yin, Z 2014, 'Continuous modification of upconversion luminescence of fluorescent dye in the crystalline colloidal arrays', Colloid and Polymer Science, vol. 292, no. 3, pp. 613-617.
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The crystalline colloidal arrays with controllable photonic bandgaps were prepared by the change of volume fraction of the polystyrene microspheres. Upconversion emission property of fluorescent dye has investigated in crystalline colloidal array, and continuous modification of the upconversion emission of fluorescent dye was observed. A significant suppression of upconversion emission of dye in the range of the photonic bandgap as well as enhancement at the bandgap edge was obtained in the crystalline colloidal arrays. In addition, upconversion emission of dye was also enhanced when the excited light overlapped with the long or short bandgap edge of the crystalline colloidal arrays, which is due to slow photons effect near the edges of a photonic bandgap. The continuous modification and enhancement of upconversion emission may be important for the development of low-threshold upconversion lasers and displays. © 2013 Springer-Verlag.
LIAO, J, YANG, Z, WU, H, LAI, S, QIU, J, SONG, Z, YANG, Y, ZHOU, D & YIN, Z 2014, 'UPCONVERSION LUMINESCENCE ENHANCEMENT OF NaYF4:Yb3+, Er3+ NANOPARTICLES ON INVERSE OPAL SURFACE', Surface Review and Letters, vol. 21, no. 01, pp. 1450017-1450017.
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LaPO 4 inverse opal photonic crystals with different photonic band gaps were fabricated by template-assisted method. The Yb 3+/ Er 3+ co-doped NaYF 4 nanoparticles were deposited on the surfaces of the inverse opals, and their up-conversion emission properties were investigated. The upconversion emissions of Yb 3+/ Er 3+ co-doped NaYF 4 nanoparticles on the inverse opal surfaces have been enhanced when the upconversion emission bands of the nanoparticles are in the range of photonic band gaps of the inverse opals, which is attributed to an efficient and selective reflection of photonic band gaps.
Lin, G, Makarov, D, Melzer, M, Si, W, Yan, C & Schmidt, OG 2014, 'A highly flexible and compact magnetoresistive analytic device', Lab Chip, vol. 14, no. 20, pp. 4050-4058.
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A highly flexible GMR-based microfluidic analytic device was demonstrated to analyze droplets of various dimensions and different concentrations of magnetic contents.
Liu, H, Wang, H, Xu, Y, Guo, R, Wen, S, Huang, Y, Liu, W, Shen, M, Zhao, J, Zhang, G & Shi, X 2014, 'Lactobionic Acid-Modified Dendrimer-Entrapped Gold Nanoparticles for Targeted Computed Tomography Imaging of Human Hepatocellular Carcinoma', ACS Applied Materials & Interfaces, vol. 6, no. 9, pp. 6944-6953.
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Liu, W, Wen, S, Jiang, L, An, X, Zhang, M, Wang, H, Zhang, Z, Zhang, G & Shi, X 2014, 'PLGA Hollow Microbubbles Loaded with Iron Oxide Nanoparticles and Doxorubicin for Dual-mode US/MR Imaging and Drug Delivery', Current Nanoscience, vol. 10, no. 4, pp. 543-552.
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Liu, W, Wen, S, Shen, M & Shi, X 2014, 'Doxorubicin-loaded poly(lactic-co-glycolic acid) hollow microcapsules for targeted drug delivery to cancer cells', New J. Chem., vol. 38, no. 8, pp. 3917-3924.
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Poly(lactic-co-glycolic acid) hollow microcapsules loaded with doxorubicin can be assembled with folate-functionalized polyethyleneimine for targeted drug delivery to cancer cells.
Liu, Z, Xing, D, Su, QP, Zhu, Y, Zhang, J, Kong, X, Xue, B, Wang, S, Sun, H, Tao, Y & Sun, Y 2014, 'Super-resolution imaging and tracking of protein–protein interactions in sub-diffraction cellular space', Nature Communications, vol. 5, no. 1, pp. 1-8.
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AbstractImaging the location and dynamics of individual interacting protein pairs is essential but often difficult because of the fluorescent background from other paired and non-paired molecules, particularly in the sub-diffraction cellular space. Here we develop a new method combining bimolecular fluorescence complementation and photoactivated localization microscopy for super-resolution imaging and single-molecule tracking of specific protein–protein interactions. The method is used to study the interaction of two abundant proteins, MreB and EF-Tu, in Escherichia coli cells. The super-resolution imaging shows interesting distribution and domain sizes of interacting MreB–EF-Tu pairs as a subpopulation of total EF-Tu. The single-molecule tracking of MreB, EF-Tu and MreB–EF-Tu pairs reveals intriguing localization-dependent heterogonous dynamics and provides valuable insights to understanding the roles of MreB–EF-Tu interactions.
Lu, Y, Lu, J, Zhao, J, Cusido, J, Raymo, FM, Yuan, J, Yang, S, Leif, RC, Huo, Y, Piper, JA, Paul Robinson, J, Goldys, EM & Jin, D 2014, 'On-the-fly decoding luminescence lifetimes in the microsecond region for lanthanide-encoded suspension arrays', Nature Communications, vol. 5, no. 1, pp. 1-8.
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Significant multiplexing capacity of optical time-domain coding has been recently demonstrated by tuning luminescence lifetimes of the upconversion nanoparticles called 'τ-Dots'. It provides a large dynamic range of lifetimes from microseconds to milliseconds, which allows creating large libraries of nanotags/microcarriers. However, a robust approach is required to rapidly and accurately measure the luminescence lifetimes from the relatively slow-decaying signals. Here we show a fast algorithm suitable for the microsecond region with precision closely approaching the theoretical limit and compatible with the rapid scanning cytometry technique.We exploit this approach to further extend optical time-domain multiplexing to the downconversion luminescence, using luminescence microspheres wherein lifetimes are tuned through luminescence resonance energy transfer.We demonstrate real-time discrimination of these microspheres in the rapid scanning cytometry, and apply them to the multiplexed probing of pathogen DNA strands. Our results indicate that tunable luminescence lifetimes have considerable potential in high-throughput analytical sciences. © 2014 Macmillan Publishers Limited. All rights reserved.
Lu, Y, Zhao, J, Zhang, R, Liu, Y, Liu, D, Goldys, EM, Yang, X, Xi, P, Sunna, A, Lu, J, Shi, Y, Leif, RC, Huo, Y, Shen, J, Piper, JA, Robinson, JP & Jin, D 2014, 'Tunable lifetime multiplexing using luminescent nanocrystals', Nature Photonics, vol. 8, no. 1, pp. 32-36.
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Optical multiplexing plays an important role in applications such as optical data storage, document security, molecular probes and bead assays for personalized medicine. Conventional fluorescent colour coding is limited by spectral overlap and background interference, restricting the number of distinguishable identities. Here, we show that tunable luminescent lifetimes τ in the microsecond region can be exploited to code individual upconversion nanocrystals. In a single colour band, one can generate more than ten nanocrystal populations with distinct lifetimes ranging from 25.6 μs to 662.4 μs and decode their well-separated lifetime identities, which are independent of both colour and intensity. Such 'τ-dots' potentially suit multichannel bioimaging, high-throughput cytometry quantification, high-density data storage, as well as security codes to combat counterfeiting. This demonstration extends the optical multiplexing capability by adding the temporal dimension of luminescent signals, opening new opportunities in the life sciences, medicine and data security. © 2013 Macmillan Publishers Limited. All rights reserved.
Martinac, B, Nomura, T, Chi, G, Petrov, E, Rohde, PR, Battle, AR, Foo, A, Constantine, M, Rothnagel, R, Carne, S, Deplazes, E, Cornell, B, Cranfield, CG, Hankamer, B & Landsberg, MJ 2014, 'Bacterial Mechanosensitive Channels: Models for Studying Mechanosensory Transduction', ANTIOXIDANTS & REDOX SIGNALING, vol. 20, no. 6, pp. 952-969.
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Significance: Sensations of touch and hearing are manifestations of mechanical contact and air pressure acting on touch receptors and hair cells of the inner ear, respectively. In bacteria, osmotic pressure exerts a significant mechanical force on their cellular membrane. Bacteria have evolved mechanosensitive (MS) channels to cope with excessive turgor pressure resulting from a hypo-osmotic shock. MS channel opening allows the expulsion of osmolytes and water, thereby restoring normal cellular turgor and preventing cell lysis. Recent Advances: As biological force-sensing systems, MS channels have been identified as the best examples of membrane proteins coupling molecular dynamics to cellular mechanics. The bacterial MS channel of large conductance (MscL) and MS channel of small conductance (MscS) have been subjected to extensive biophysical, biochemical, genetic, and structural analyses. These studies have established MscL and MscS as model systems for mechanosensory transduction. Critical Issues: In recent years, MS ion channels in mammalian cells have moved into focus of mechanotransduction research, accompanied by an increased awareness of the role they may play in the pathophysiology of diseases, including cardiac hypertrophy, muscular dystrophy, or Xerocytosis. Future Directions: A recent exciting development includes the molecular identification of Piezo proteins, which function as nonselective cation channels in mechanosensory transduction associated with senses of touch and pain. Since research on Piezo channels is very young, applying lessons learned from studies of bacterial MS channels to establishing the mechanism by which the Piezo channels are mechanically activated remains one of the future challenges toward a better understanding of the role that MS channels play in mechanobiology. Antioxid. Redox Signal. 20, 952-969. © 2014 Mary Ann Liebert, Inc.
Pant, R, Marpaung, D, Kabakova, IV, Morrison, B, Poulton, CG & Eggleton, BJ 2014, 'On-chip stimulated Brillouin Scattering for microwave signal processing and generation', Laser & Photonics Reviews, vol. 8, no. 5, pp. 653-666.
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Rong, Y, Wang, D, Wu, W, Jin, D, Kuang, T, Ni, X, Zhang, L & Lou, W 2014, 'TRAF6 is over-expressed in pancreatic cancer and promotes the tumorigenicity of pancreatic cancer cells', Medical Oncology, vol. 31, no. 11.
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Salavati-Niasari, M, Behfard, Z & Maddahfar, M 2014, 'Controllable synthesis of Bi2S3 via a simple hydrothermal approach starting from an inorganic precursor', Journal of Industrial and Engineering Chemistry, vol. 20, no. 6, pp. 4066-4075.
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Si, W, Mönch, I, Yan, C, Deng, J, Li, S, Lin, G, Han, L, Mei, Y & Schmidt, OG 2014, 'A Single Rolled‐Up Si Tube Battery for the Study of Electrochemical Kinetics, Electrical Conductivity, and Structural Integrity', Advanced Materials, vol. 26, no. 47, pp. 7973-7978.
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Sun, H-T, Zhou, J & Qiu, J 2014, 'Recent advances in bismuth activated photonic materials', Progress in Materials Science, vol. 64, pp. 1-72.
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Bismuth is one of the most thoroughly investigated main group elements, which has been regarded as ‘the wonder metal’ because of its diverse oxidation states and profound propensities to form bismuth clusters, resulting from the easy involvement in chemical combinations for the electrons in the p orbital. This peculiarity allows them to behave as smart optically active centers in diverse host materials. Remarkable progress in the research of bismuth activated photonic materials has been seen over the last ten years owing to their unique properties and important applications in areas of telecommunication, biomedicine, white light illumination and lasers. The aim of this review is to present a critical overview of the current state of the art in bismuth activated photonic materials, their features, advantages and limitations as well as the future research trends. We first shortly introduce the fundamental properties of bismuth element including principles of bismuth-related luminescence and characterization techniques available. This is followed by a detailed discussion on the recent progress in the synthesis and characterization of bismuth-activated photonic materials, with an emphasis on material systems emitting in the near-infrared (NIR) spectral region. Furthermore, we describe the representative achievements regarding their prospective applications in broadband NIR optical amplifiers, fiber lasers, bioimaging, and white light-emitting diodes. Finally, we point out what key scientific questions remain to be answered, and present our perspectives on future research trends in this exciting field of sciences.
Sun, K, Eriksson, SE, Tan, Y, Zhang, L, Arnér, ESJ & Zhang, J 2014, 'Serum thioredoxin reductase levels increase in response to chemically induced acute liver injury', Biochimica et Biophysica Acta (BBA) - General Subjects, vol. 1840, no. 7, pp. 2105-2111.
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Warkiani, ME, Guan, G, Luan, KB, Lee, WC, Bhagat, AAS, Kant Chaudhuri, P, Tan, DS-W, Lim, WT, Lee, SC, Chen, PCY, Lim, CT & Han, J 2014, 'Slanted spiral microfluidics for the ultra-fast, label-free isolation of circulating tumor cells', Lab Chip, vol. 14, no. 1, pp. 128-137.
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Warkiani, ME, Khoo, BL, Tan, DS-W, Bhagat, AAS, Lim, W-T, Yap, YS, Lee, SC, Soo, RA, Han, J & Lim, CT 2014, 'An ultra-high-throughput spiral microfluidic biochip for the enrichment of circulating tumor cells', The Analyst, vol. 139, no. 13, pp. 3245-3255.
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We demonstrate the high-throughput and high-resolution separation of rare circulating tumor cells (CTCs) from blood using a multiplexed spiral microfluidic device.
Wen, S, Zhao, Q, An, X, Zhu, J, Hou, W, Li, K, Huang, Y, Shen, M, Zhu, W & Shi, X 2014, 'Multifunctional PEGylated Multiwalled Carbon Nanotubes for Enhanced Blood Pool and Tumor MR Imaging', Advanced Healthcare Materials, vol. 3, no. 10, pp. 1568-1577.
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Wu, H, Yang, Z, Liao, J, Lai, S, Qiu, J, Song, Z, Yang, Y & Zhou, D 2014, 'Enhancement of near-infrared to near-infrared upconversion emission in the CeO_2: Er^3+, Tm^3+, Yb^3+ inverse opals', Optics Letters, vol. 39, no. 4, pp. 918-918.
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Wu, W, Yao, X, Lin, C, Jin, D, Wang, D, Lou, W & Qin, X 2014, 'Should Steroid Therapy Be Necessarily Needed for Autoimmune Pancreatitis Patients with Lesion Resected due to Misdiagnosed or Suspected Malignancy?', Gastroenterology Research and Practice, vol. 2014, pp. 1-6.
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To explore whether steroid therapy should be needed for autoimmune pancreatitis patients after operation, eight AIP patients receiving operation were enrolled in this study from January 2007 to July 2013. All patients underwent liver function, CA19-9, and contrast-enhanced CT and/or MRI. Tests of IgG and IgG4 were performed in some patients. Tests of serum TB/DB,γ-GT, andγ-globulin were undergone during the perioperative period. Six cases receiving resection were pathologically confirmed as AIP patients and two were confirmed by intraoperative biopsy. For seven patients, TB/DB level was transiently elevated 1 day or 4 days after operation but dropped below preoperative levels or to normal levels 7 days after operation, and serumγ-GT level presented a downward trend. Serumγ-globulin level exhibited a downward trend among six AIP patients after resection, while an upward trend was found in another two AIP patients receiving internal drainage. Steroid therapy was not given to all six AIP patients until two of them showed new lines of evidence of residual or extrapancreatic AIP lesion after operation, while another two cases without resection received steroid medication. Steroid therapy might not be recommended unless there are new lines of evidence of residual extrapancreatic AIP lesions after resection.
Wu, Y, Guo, R, Wen, S, Shen, M, Zhu, M, Wang, J & Shi, X 2014, 'Folic acid-modified laponite nanodisks for targeted anticancer drug delivery', J. Mater. Chem. B, vol. 2, no. 42, pp. 7410-7418.
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Folic acid-modified laponite nanodisks can be used as an efficient platform for targeted delivery of doxorubicinviaa receptor-mediated pathway.
Xie, H, Jin, D, Yu, J, Peng, T, Ding, Y, Zhou, C & Xi, P 2014, 'Schlieren confocal microscopy for phase-relief imaging', OPTICS LETTERS, vol. 39, no. 5, pp. 1238-1241.
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We demonstrate a simple phase-sensitive microscopic technique capable of imaging the phase gradient of a transparent specimen, based on the Schlieren modulation and confocal laser scanning microscopy (CLSM). The incident laser is refracted by the phase gradient of the specimen and excites a fluorescence plate behind the specimen to create a secondary illumination; then the fluoresence is modulated by a partial obstructor before entering the confocal pinhole. The quantitative relationship between the image intensity and the sample phase gradient can be derived. This setup is very easy to be adapted to current confocal setups, so that multimodality fluorescence/structure images can be obtained within a single system.
Xuefeng, X, Jian’ang, L, Xu, H, Chenye, S, Dayong, J & Wenhui, L 2014, 'Clinical characteristics and prognostic factors of patients with gastric neuroendocrine carcinoma treated with radical surgery', Chinese Medical Journal, vol. 127, no. 13, pp. 2419-2422.
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Background Gastric neuroendocrine carcinomas (g-NECs) are rare tumors that have aggressive biological behaviors and poor prognosis, but the prognostic factors of postoperative patients with g-NEC are still unclear. Our aim was to study and explore the clinical characteristics and prognostic factors of patients with g-NEC treated with radical surgery. Methods The clinical data of 43 g-NEC patients who underwent surgery from January 2002 to January 2011 at the Zhongshan Hospital of Fudan University were analyzed. Follow-up was conducted by telephone, mail, or returning visit survey. Results The sizes of the 43 neuroendocrine carcinomas (G3) were 1.5 cm × 1.5 cm × 0.5 cm to 7 cm × 8 cm × 1.5 cm. Eight NECs were localized, and 35 had lymph node involvement, of which 1 also had hepatic metastasis. At the end of the follow-up, the follow-up rate was 97.7% (42/43), and the median follow-up time was 22.2 months. The median overall survival of g-NEC patients was 36.5 months, and the 1-, 3-, and 5-year overall survival rates were 86.0%, 51.6%, and 36.7%, respectively. Sex (P <0.05) and lymph node involvement (P <0.05) were prognostic factors of postoperative g-NEC patients, among which sex was an independent prognostic factor (P <0.05), as a survival advantage of female patients over male was observed. Conclusions Most of the g-NECs were diagnosed at an advanced stage. The prognosis of g-NECs was related with sex and lymph node involvement, of which...
Yang, Z, Liao, J, Wang, T, Wu, H, Qiu, J, Song, Z & Zhou, D 2014, 'Ultraviolet long afterglow emission in Bi<SUP>3</SUP><SUP>+</SUP> doped CdSiO<SUB>3</SUB> phosphors', Materials Express, vol. 4, no. 2, pp. 172-176.
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Ye, Z, Zhang, R, Song, B, Dai, Z, Jin, D, Goldys, EM & Yuan, J 2014, 'Development of a functional ruthenium(ii) complex for probing hypochlorous acid in living cells', Dalton Transactions, vol. 43, no. 22, pp. 8414-8414.
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A functional ruthenium(ii) complex, [Ru(bpy)2(AN-bpy)](PF 6)2 (bpy: 2,2′-bipyridine, AN-bpy: 4-methyl-4′-(4-amino-3-nitro-phenoxy-methylene)-2,2′-bipyridine), has been designed and synthesized as a turn-on luminescent probe for the imaging of hypochlorous acid (HOCl) in living cells. Due to the intramolecular photoinduced electron transfer (PET), the ruthenium(ii) complex itself is almost non-luminescent. However, it can specifically and rapidly react with HOCl in aqueous media to afford a highly luminescent derivative, [Ru(bpy) 2(HM-bpy)](PF6)2 (HM-bpy: 4-hydroxymethyl- 4′-methyl-2,2′-bipyridine), accompanied by a 110-fold luminescence enhancement. Taking advantage of high specificity and sensitivity, and excellent photophysical properties of the ruthenium(ii) complex probe, [Ru(bpy) 2(AN-bpy)](PF6)2 was successfully applied to the luminescence imaging of the exogenous HOCl in living HeLa cells and the endogenous HOCl in porcine neutrophils. The results corroborate that indeed [Ru(bpy)2(AN-bpy)](PF6)2 is a useful luminescent probe for the monitoring of HOCl in biological systems. © the Partner Organisations 2014.
Zhang, L, Wei, Y & Zhang, J 2014, 'Novel Mechanisms of Anticancer Activities of Green Tea Component Epigallocatechin- 3-Gallate', Anti-Cancer Agents in Medicinal Chemistry, vol. 14, no. 6, pp. 779-786.
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Zhang, L, Zheng, X, Deng, W, Lu, Y, Lechevallier, S, Ye, Z, Goldys, EM, Dawes, JM, Piper, JA, Yuan, J, Verelst, M & Jin, D 2014, 'Practical Implementation, Characterization and Applications of a Multi-Colour Time-Gated Luminescence Microscope', Scientific Reports, vol. 4, no. 1, pp. 1-6.
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AbstractTime-gated luminescence microscopy using long-lifetime molecular probes can effectively eliminate autofluorescence to enable high contrast imaging. Here we investigate a new strategy of time-gated imaging for simultaneous visualisation of multiple species of microorganisms stained with long-lived complexes under low-background conditions. This is realized by imaging two pathogenic organisms (Giardia lamblia stained with a red europium probe and Cryptosporidium parvum with a green terbium probe) at UV wavelengths (320–400 nm) through synchronization of a flash lamp with high repetition rate (1 kHz) to a robust time-gating detection unit. This approach provides four times enhancement in signal-to-background ratio over non-time-gated imaging, while the average signal intensity also increases six-fold compared with that under UV LED excitation. The high sensitivity is further confirmed by imaging the single europium-doped Y2O2S nanocrystals (150 nm). We report technical details regarding the time-gating detection unit and demonstrate its compatibility with commercial epi-fluorescence microscopes, providing a valuable and convenient addition to standard laboratory equipment.
Zhang, Y, Zhang, L, Deng, R, Tian, J, Zong, Y, Jin, D & Liu, X 2014, 'Multicolor Barcoding in a Single Upconversion Crystal', Journal of the American Chemical Society, vol. 136, no. 13, pp. 4893-4896.
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We report the synthesis of luminescent crystals based on hexagonal-phase NaYF4 upconversion microrods. The synthetic procedure involves an epitaxial end-on growth of upconversion nanocrystals comprising different lanthanide activators onto the NaYF4 microrods. This bottom-up method readily affords multicolor-banded crystals in gram quantity by varying the composition of the activators. Importantly, the end-on growth method using one-dimensional microrods as the template enables facile multicolor tuning in a single crystal, which is inaccessible in conventional upconversion nanoparticles. We demonstrate that these novel materials offer opportunities as optical barcodes for anticounterfeiting and multiplexed labeling applications. © 2014 American Chemical Society.
Zheng, S, Chen, W, Tan, D, Zhou, J, Guo, Q, Jiang, W, Xu, C, Liu, X & Qiu, J 2014, 'Lanthanide-doped NaGdF4 core–shell nanoparticles for non-contact self-referencing temperature sensors', Nanoscale, vol. 6, no. 11, pp. 5675-5679.
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A self-referencing nanothermometer is developed based on near infrared laser stimulated visible upconversion from lanthanide-activated core–shell nanoparticles.
Zhou, B, Zheng, L, Peng, C, Li, D, Li, J, Wen, S, Shen, M, Zhang, G & Shi, X 2014, 'Synthesis and Characterization of PEGylated Polyethylenimine-Entrapped Gold Nanoparticles for Blood Pool and Tumor CT Imaging', ACS Applied Materials & Interfaces, vol. 6, no. 19, pp. 17190-17199.
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Zhu, J, Zheng, L, Wen, S, Tang, Y, Shen, M, Zhang, G & Shi, X 2014, 'Targeted cancer theranostics using alpha-tocopheryl succinate-conjugated multifunctional dendrimer-entrapped gold nanoparticles', Biomaterials, vol. 35, no. 26, pp. 7635-7646.
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Büttner, TFS, Hudson, DD, Kabakova, IV & Eggleton, BJ 1970, 'Phase-locked, multiwavelength, distributed feedback brillouin laser', Optics InfoBase Conference Papers.
Büttner, TFS, Kabakova V., IV, Hudson D., DD, Pant, R, Poulton G., CG, Judge C., AC & Eggleton J., BJ 1970, 'Phase-locking in multi-frequency brillouin oscillator via four-wave mixing', Optics InfoBase Conference Papers.
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We report the experimental demonstration and numerical modeling of phase-locking cascaded Stokes waves generated by Stimulated Brillouin Scattering via Kerr nonlinear four-wave mixing in a short, chalcogenide fiber resonator, producing phase-locked trains of picosecond pulses. © 2014 OSA.
Büttner, TFS, Kabakova, IV, Hudson, DD, Pant, R, Poulton, CG, Judge, AC & Eggleton, BJ 1970, 'Phase-locking in multi-frequency brillouin oscillator via four-wave mixing', Conference on Lasers and Electro-Optics Europe - Technical Digest, Conference on Lasers and Electro-Optics (CLEO) - Laser Science to Photonic Applications, IEEE, San Jose, CA, USA.
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We report the experimental demonstration and numerical modeling of phase-locking cascaded Stokes waves generated by Stimulated Brillouin Scattering via Kerr nonlinear four-wave mixing in a short, chalcogenide fiber resonator, producing phase-locked trains of picosecond pulses.
Büttner, TFS, Kabakova, IV, Hudson, DD, Pant, R, Poulton, CG, Judge, AC & Eggleton, BJ 1970, 'Phase-Locking in Multi-Frequency Brillouin Oscillator via Four-Wave Mixing', CLEO: 2014, CLEO: QELS_Fundamental Science, OSA.
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Büttner, TFS, Kabakova, IV, Merklein, M, Hudson, DD, Choi, D-Y, Luther-Davies, B, Madden, SJ & Eggleton, BJ 1970, 'Phase-locked Cascaded Stimulated Brillouin Scattering and Pulse Train Generation on a Photonic Chip', Advanced Photonics, Nonlinear Photonics, OSA.
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We experimentally demonstrate the generation of phase-locked Brillouin-frequency combs and gigahertz repetition rate pulse trains via the interplay of stimulated Brillouin scattering and Kerr-nonlinear four-wave mixing on a chalcogenide chip.
Büttner, TFS, Kabakova, IV, Merklein, M, Hudson, DD, Choi, DY, Luther-Davies, B, Madden, SJ & Eggleton, BJ 1970, 'Phase-locked cascaded stimulated Brillouin scattering and pulse train generation on a photonic chip', Nonlinear Photonics, NP 2014.
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We experimentally demonstrate the generation of phase-locked Brillouin-frequency combs and gigahertz repetition rate pulse trains via the interplay of stimulated Brillouin scattering and Kerr-nonlinear four-wave mixing on a chalcogenide chip.
Cao, X, Tao, L, Wen, S, Hou, W & Shi, X 1970, 'Hyaluronic acid-modified multiwalled carbon nanotubes as drug delivery carrier for targeted cancer therapy', ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 247th National Spring Meeting of the American-Chemical-Society (ACS), AMER CHEMICAL SOC, Dallas, TX.
Cranfield, CG, Carne, S, Alkhamici, H, Duckworth, P, Lacey, E, Martinac, B & Cornell, B 1970, 'Screening the Insertion of Families of Bioactive Microbial Metabolites into Tethered Bilayer Lipid Membranes (TBLMS)', BIOPHYSICAL JOURNAL, pp. 294A-294A.
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Hu, K, Kabakova, IV, Büttner, TFS, Lefrancois, S, Hudson, DD, He, S & Eggleton, BJ 1970, 'Low-threshold Brillouin laser at 2 μm based on suspended-core chalcogenide fiber', Advanced Solid State Lasers, Advanced Solid State Lasers, OSA.
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We present the first demonstration of a 2 μm Brillouin laser based on chalcogenide fiber. A record-low lasing threshold of 52 mW is achieved, which is about 10 times lower than the earlier reports.
Hu, K, Kabakova, IV, Büttner, TFS, Lefrancois, S, Hudson, DD, He, S & Eggleton, BJ 1970, 'Low-threshold Brillouin laser at 2 μm based on suspended-core chalcogenide fiber.', Opt Lett, OPTICAL SOC AMER, United States, pp. 4651-4654.
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We present, to the best of our knowledge, the first demonstration of a 2 μm Brillouin laser based on a thulium-doped fiber pump and a chalcogenide fiber. A short 1.5 m piece of suspended-core chalcogenide As38Se62 fiber is employed as a gain medium, taking advantage of its small effective mode area and high Brillouin gain coefficient. A record-low lasing threshold of 52 mW is achieved, which is about 10 times lower than previously demonstrated in silica fiber cavities.
Jin, G, Han, X, Jin, D, Hu, X, Wang, D, Shao, C, Ji, Y, Zhang, Y, Xu, X & Lou, WB 1970, 'Characteristics and Determinants of Long-Term Outcome for Resectable Sporadic Non-Functional Pancreatic Neuroendocrine Tumors (NF-PNETs): Updated 16-year Experience from Two Large Chinese Centers', NEUROENDOCRINOLOGY, 11th Annual ENETS Conference for the Diagnosis and Treatment of Neuroendocrine Tumor Disease, KARGER, SPAIN, Barcelona, pp. 286-286.
Kabakova, IV, Büttner, TFS, Merklein, M, Choi, DY, Madden, S, Luther-Davies, B & Eggleton, BJ 1970, 'Advances in chip-based Brillouin sources', 2014 OptoElectronics and Communication Conference, OECC 2014 and Australian Conference on Optical Fibre Technology, ACOFT 2014, 19th OptoElectronics and Communication Conference (OECC) / 39th Australian Conference on Optical Fibre Technology (ACOFT), IEEE, Engineers Australia, Melbourne, AUSTRALIA, pp. 335-336.
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We present single- and multifrequency Brillouin lasers based on chalcogenide photonic chip. The high Brillouin gain of chalcogenide and the small waveguide cross-section make it possible to achieve lasing in a few centimeter long waveguide. © 2014 Engineers Australia.
Khoo, BL, Warkiani, ME, Guan, G, Tan, DS-W, Lim, AST, Lim, W-T, Yap, YS, Lee, SC, Soo, RA, Han, J & Lim, CT 1970, 'Ultra-High Throughput Enrichment of Viable Circulating Tumor Cells', IFMBE Proceedings, Springer International Publishing, pp. 1-4.
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© Springer International Publishing Switzerland 2014. Detection, enumeration and characterization of rare circulating tumor cells (CTCs) from the peripheral blood of cancer patients potentially provide critical insights into tumor biology and is promising for cancer diagnosis and prognosis. Here, we present a novel multiplexed spiral microfluidic device for ultra-high throughput, label-free enrichment of CTCs from clinically relevant blood volumes. The fast processing time of the technique (7.5 mL blood in < 5 min) and high sensitivity of the device lends itself to a broad range of potential genomic and transcriptomic applications. The method can specifically separate and preserve all fractions of blood (i.e., plasma, CTCs and PBMC) for diverse downstream analysis. CTCs were detected from 100% (10/10) of blood samples collected from patients with advanced stage metastatic breast (12- 56 CTC/ml) or lung cancer (30-153 CTC/ml). Cancer cells were characterized with immunostaining and fluorescence in situ hybridization (FISH) (HER2/neu). Retrieved cells were unlabelled and hence more viable for propagation and other informative analysis such as the next generation sequencing (NGS) to guide treatment and individualized patient care.
Merklein, M, Kabakova, IV, Buettner, T, Madden, S, Luther-Davies, B, Choi, D-Y & Eggleton, BJ 1970, 'Slow-light enhanced Brillouin frequency comb generation on a chip', CLEO: 2014, CLEO: Science and Innovations, OSA.
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Merklein, M, Kabakova, IV, Buettner, TFS, Madden, SJ, Luther-Davies, B, Choi, DY & Eggleton, BJ 1970, 'Slow-light enhanced brillouin frequency comb generation on a chip', Conference on Lasers and Electro-Optics Europe - Technical Digest.
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We present the experimental observation of the slow-light enhancement effect on the generation of a frequency comb formed by stimulated Brillouin scattering (SBS) in a chip scale As2S3 rib waveguide.
Soeriyadi, AH, Zhu, Y, Reece, P & Gooding, JJ 1970, 'Modification of porous silicon rugate filters through thiol-yne photochemistry', AIP Conference Proceedings, 5TH NANOSCIENCE AND NANOTECHNOLOGY SYMPOSIUM (NNS2013), AIP Publishing LLC, Surabaya, INDONESIA, pp. 35-38.
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Warkiani, ME & Gong, HQ 1970, 'Micro-fabricated Membranes with Regular Pores for Efficient Pathogen Removal', IFMBE Proceedings, Springer International Publishing, pp. 424-427.
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© Springer International Publishing Switzerland 2014. Rapid and accurate detection of pathogenic bacteria in drinking-water systems is a challenging problem. Filtration based concentration techniques have been widely used for isolation and recovery of C. parvum and Giardia (oo)cysts into small volumes for downstream analysis. Micro-fabricated membranes that contain pores with the same size and shape have been shown to be a good choice for efficient pathogen removal. In this study, a robust isoporous membrane was fabricated and validated for concentration and recovery of C. parvum and Giardia (oo)cysts from tap-water according to the EPA standard protocol. Microfiltration results of the isoporous polymeric microfilter revealed that using a simple backflushing procedure, more than 90% of the C. parvum and Giardia (oo)cysts spiked in the tap-water samples can be recovered, showing greater performance than available commercial microfilters. This research demonstrated the potential application of micro-fabricated filters with regular pores for large-scale filtration and monitoring of C. parvum and Giardia (oo)cysts contamination in drinking-water distribution systems.
Warkiani, ME, Tay, AKP, Guan, G & Han, J 1970, 'Next-generation microfilter: Large scale, continuous mammalian cell retention for perfusion bioreactors', 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, pp. 2474-2476.
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In this study, we report on the development of the first membrane-less, clog-free microfiltration platform for ultra-high throughput (up to 1000 mL/min) cell separation, using a massively-multiplexed array of inertial microfluidic cell sorting channels. Our developed system consists of multiple layer (10∼20) of PDMS sheets with embossed microchannels (i.e., ∼ 200 individual spirals) bonded together for continuous size-based cell sorting from a large volume of biological fluid. Subsequent perfusion culture experiments using the cell retention system show the potential to significantly enhance overall efficiency of perfusion cell culture.
Warkiani, ME, Tay, AKP, Khoo, BL, Xu, X, Lim, CT & Han, J 1970, 'Enabling reliable detection of low abundance malaria parasites from blood using inertial microfluidics', 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, pp. 1157-1159.
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In this paper, a highly efficient inertial microfluidic device was described to enable the enrichment and purification of malaria parasites from blood, which leads to more reliable and specific PCR-based malaria detection. The device makes use of equilibrium between shear-modulated inertial lift and wall-induced lift forces to remove WBCs, thereby facilitating malaria parasite enrichment. The cascaded system is able to process 1 mL of lysed blood in 15 min, with the WBC depletion efficiency of 99.99% which is higher than any commercially available kit (i.e., CD-45 coated beads) for this purpose. Parasite densities ranging from 103 to 104 P. falciparum parasites per mL (∼ 2 per μL) have been quantified in whole blood using quantitative PCR. Obtained results revealed that the sample preparation using the inertial microfluidic device can significantly enhance the PCR results and make it more reliable by removing most of the unwanted genomic materials and PCR inhibitors.
