Deng, W, Drozdowicz-Tomsia, K, Jin, D & Goldys, EM 2009, 'Enhanced Flow Cytometry-Based Bead Immunoassays Using Metal Nanostructures', Analytical Chemistry, vol. 81, no. 17, pp. 7248-7255.
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Jin, D, Piper, JA, Leif, RC, Yang, S, Ferrari, BC, Yuan, J, Wang, G, Vallarino, LM & Williams, JW 2009, 'Time-gated flow cytometry: an ultra-high selectivity method to recover ultra-rare-event μ-targets in high-background biosamples', Journal of Biomedical Optics, vol. 14, no. 2, pp. 024023-024023.
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Jin, D, Rong, Y, Lou, W, Qin, X, Wu, W, Nin, X, Nin, X, Wang, D, Kuang, T & Qin, Y 2009, 'Therapeutic vaccination against advanced pancreatic cancer by autologous dendritic cells pulsed with a MUC1 peptide: Preclinical results of a clinical phase I trial', Journal of Clinical Oncology, vol. 27, no. 15_suppl, pp. e15652-e15652.
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e15652 Background: Pancreatic ductal adenocarcinoma has a particularly poor prognosis. Therefore, novel therapeutic strategies such as immunotherapy are required. The aim of the present phase I study was to evaluate the safety, immune responses and clinical activity of a vaccine based on autologous dendritic cells (DC) pulsed with a specific MUC1 peptide in advanced pancreatic cancer patients. Methods: Five patients who had pancreatic cancer ductal adenocarcinoma expressing MUC1 in stage of III/IV were enrolled to the clinical trial. Patients underwent leukapheresis to generate dendritic cells by culture in vitro with granulocyte macrophage colony-stimulating factor and interleukin-4 for 5 days. Dendritic cells were then pulsed overnight with MUC1 peptide (GVTSAPDTRPAPGSTAPPAH) and harvested for vaccination. Dendritic cells (3×106-6×106) were injected intradermally every 2 weeks for 3–4 times. Results: All patients remained with progressive disease. Four patients developed strong T-cell IFN-γ and Granzyme B Elispot responses to the vaccine. Most interestingly, the patient who was treated with the highest number of DC(6×106) had more number of CTL than other patients and showed delayed-type hypersensitivity responses at injection sites and this patient stopped application of the analgetics. Another patient with relapsed pancreatic cancer who had finished the 4 times of vaccination and then followed 6 times of chemotherapy with Gemcitabine had a surprisingly long term of survival of 12 month. No evidence of significant treatment related toxicity or auto-immunity was observed. Conclusions: This study showed the safety and clinical response of MUC1 peptide-pulsed dendritic cell therapy for patients with advanced pancreatic cancer. It confirms the capability of this DC vaccine to stimulate an immune response in patients with pancreatic cancer even in the presence of a l...
Kabakova, IV, Corcoran, B, Bolger, JA, de Sterke, M & Eggleton, BJ 2009, 'All-optical self-switching in optimized phase-shifted fiber Bragg grating', Optics Express, vol. 17, no. 7, pp. 5083-5083.
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KUANG, TT, JIN, DY, WANG, DS, XU, XF, NI, XL, WU, WC & LOU, WH 2009, 'Clinical epidemiological analysis of the relationship between pancreatic cancer and diabetes mellitus: Data from a single institution in China', Journal of Digestive Diseases, vol. 10, no. 1, pp. 26-29.
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OBJECTIVE: To investigate the relationship between pancreatic cancer (PC) and diabetes mellitus.METHODS: All PC patients diagnosed and treated at Zhongshan hospital from January 1991 to December 2007 were retrospectively analyzed. During this period, 770 non‐digestive tract, non‐neoplastic and non‐hormone‐related patients matched for sex and age were collected as controls. The incidence of diabetes mellitus between the two groups was compared.RESULTS: Between the PC group and the control group, sex and age of the patients were well matched. The incidence of diabetes mellitus was 34.63% in the PC group and 8.83% in the control group (P < 0.001, RR = 5.19). In the PC group there was no correlation between age, sex, site of the cancer, tumor differentiation, lymph node metastasis, TNM staging and the incidence of diabetes mellitus. In this group with diabetes, 74.56% experienced onset within two years of cancer diagnosis. Of the control patients, 57.35% had had diabetes for under 2 years (P = 0.009, RR = 2.18). In the PC group with diabetes, 5.9% had had diabetes for more than 10 years while compared with 8.8% of the controls (P = 0.42).CONCLUSION: Whether diabetes mellitus is a result of or a risk factor for PC is still unclear. The incidence of diabetes mellitus is much higher in the PC patients. The onset of diabetes mellitus in adults might be an alerting factor that could lead to an early diagnosis of pancreatic cancer.
Leif, RC, Yang, S, Jin, D, Piper, J, Vallarino, LM, Williams, JW & Zucker, RM 2009, 'Calibration beads containing luminescent lanthanide ion complexes', Journal of Biomedical Optics, vol. 14, no. 2, pp. 024022-024022.
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Rong, Y, Jin, D, Wu, W, Lou, W, Wang, D, Kuang, T, Ni, X & Qin, X 2009, 'Induction of protective and therapeutic anti-pancreatic cancer immunity using a reconstructed MUC1 DNA vaccine', BMC Cancer, vol. 9, no. 1.
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Abstract Background Pancreatic cancer is a common, highly lethal disease with a rising incidence. MUC1 is a tumor-associated antigen that is over-expressed in pancreatic adenocarcinoma. Active immunotherapy that targets MUC1 could have great treatment value. Here we investigated the preventive and therapeutic effect of a MUC1 DNA vaccine on the pancreatic cancer. Methods MUC1-various tandem repeat units(VNTR) DNA vaccine was produced by cloning one repeat of VNTR and inserting the cloned gene into the pcDNA3.1. In the preventive group, female C57BL/6 mice were immunized with the vaccine, pcDNA3.1 or PBS; and challenged with panc02-MUC1 or panc02 cell. In the therapeutic group the mice were challenged with panc02-MUC1 or panc02 cell, and then immunized with the vaccine, pcDNA3.1 or PBS. The tumor size and the survival time of the animals were compared between these groups. Results The DNA vaccine pcDNA3.1-VNTR could raise cytotoxic T lymphocyte (CTL) activity specific for MUC1. In the preventive experiment, the mice survival time was significantly longer in the vaccine group than in the control groups (P < 0.05). In the therapeutic experiment, the DNA vaccine prolonged the survival time of the panc02-MUC1-bearing mice (P < 0.05). In both the preventive and therapeutic experiments, the tumor size was significantly less in the vaccine group than in the control groups (P < 0.05). This pcDNA3.1-VNTR vaccine, however, could not prevent the mice attacked by panc02 cells and had no therapeutic effect on the mice attacked by panc02 cells. <...
Shi, C, Jin, D, Xu, B & Lou, W 2009, 'Value of monitoring postoperative intra‐abdominal drainage fluid for the diagnosis of postoperative pancreatic fistula: Results of a prospective study in 134 patients', Surgical Practice, vol. 13, no. 4, pp. 102-107.
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Objective: To evaluate the value of monitoring postoperative intra‐abdominal drainage in the diagnosis and treatment of postoperative pancreatic fistula (POPF).Methods: Intra‐abdominal drainage adjacent to the pacreaticoenteric anastomosis was prospectively collected from 134 patients accepted for pancreatic surgery in our department between November 2005 and August 2007 to determine the concentration of amylase and bacteriology. Also, the clinical outcome of each patient was recorded. The international study group definition was used to diagnose POPF.Results: The overall incidence of POPF was 39.6% in 53 patients diagnosed as POPF, among which 50 patients (37.3%, 50/134) were graded as I/II of POPF. The rising trend of the amylase level in the drainage fluid (P = 0.001), the texture of the pancreatic remnant (P = 0.042), and the amylase level on the 3rd and 7th postoperative days demonstrated good diagnostic values in univariate analysis (P < 0.001). The amylase value in drains on the 3rd postoperative day (POD3) and the 7th postoperative day (POD7) were the predicting factors (P < 0.001) of POPF by multivariate analysis.Conclusions: The amylase values in drains on the 3rd postoperative day and on the 7th postoperative day (POD7) were both valuable factors in the diagnoses of POPF. It is very important to monitor the dynamic change of amylase concentration in the drainage, not the result of a single test. These clinical factors could help us to detect the incidence of POPF earlier.
Song, C, Ye, Z, Wang, G, Jin, D, Yuan, J, Guan, Y & Piper, J 2009, 'Preparation and time-gated luminescence bioimaging application of ruthenium complex covalently bound silica nanoparticles', Talanta, vol. 79, no. 1, pp. 103-108.
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Teymourtash, AR & Ebrahimi Warkiani, M 2009, 'Natural convection over a non-isothermal vertical flat plate in supercritical fluids', Scientia Iranica, vol. 16, no. 6 B, pp. 470-478.
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In many applications, convection heat transfer is coupled with conduction and radiation heat transfer, which generate temperature gradients along the walls and may greatly affect natural convection heat transfer. The main objective of this study is to calculate the heat-transfer characteristics for natural convection from a non-isothermal vertical flat plate into a supercritical fluid. The influence of the non-uniformity of wall temperature on the heat transfer by natural convection along a vertical plate, having a linearly distributed temperature (characterized by the slope S) is also investigated. The thermal expansion coefficient is considered as a function of the temperature, the pressure, the van der Waals constants and the compressibility factor. The trends of the curves obtained with this equation and with values from tables of thermodynamic properties were similar and diverged at a critical point. These features confirmed the validity of this equation. Then, the governing systems of partial differential equations are solved numerically using the finite difference method. The local Nusselt number was then calculated and plotted as a function of the local Rayleigh number. It was observed that a positive slope of temperature distribution increases the heat transfer rate and a negative slope decreases it. © Sharif University of Technology, December 2009.
Teymourtash, AR & Warkiani, ME 2009, 'Natural Convection over a Non-Isothermal Vertical Flat Plate in Supercritical Fluids', SCIENTIA IRANICA TRANSACTION B-MECHANICAL ENGINEERING, vol. 16, no. 6, pp. 470-478.
Wu, J, Ye, Z, Wang, G, Jin, D, Yuan, J, Guan, Y & Piper, J 2009, 'Visible-light-sensitized highly luminescent europium nanoparticles: preparation and application for time-gated luminescence bioimaging', Journal of Materials Chemistry, vol. 19, no. 9, pp. 1258-1258.
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Wu, W, Jin, D, Lou, W, Fan, J, Wang, D & Qin, X 2009, 'A novel recombinant tandem repeat DNA vaccine targeting at MUC1', Journal of Clinical Oncology, vol. 27, no. 15_suppl, pp. 3066-3066.
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3066 Background: Tandem repeat (TR) is the key epitope of mucin 1 (MUC1) for inducing cytotoxic T lymphocytes (CTL) to kill the tumor cells specifically. A novel recombinant TR DNA vaccine was constructed to study its induced immune responses. Methods: A recombinant human TR (rhTR) gene encoding a single TR polypeptide of MUC1 was synthesized and cloned into the multiple cloning sites of plasmid pcDNA3.1/Myc-his (+) A to construct the recombinant plasmid pcDNA3.1-TR/Myc-his (+) A (pTR plasmid). Expression of pTR plasmid was confirmed by transfection assay and Western blot analysis. C57BL/6 (H-2b) mice were immunized with pTR plasmid (n=15) by tibial muscle injection. Mice inoculated with the empty vector (EV group, n=15) and 0.9% NaCl solution (NS group, n=15) were used as vector and blank control respectively. Four weeks later, all mice were immunized again. Specific antibody detection and cytotoxic assay were used to evaluate the vaccine-induced TR specific immune responses. Results: DNA sequencing confirmed that the pTR plasmid was exactly constructed. Transfection assay and Western blot analysis found that the transfected COS7 cells expressed TR polypeptide of MUC1 48 hours after transfection. Cytotoxic assay showed that immunization with pTR plasmid into C57BL/6 mice resulted in more efficient induction of CTL specific cytolysis against TR polypeptide than that of EV group and NS group (p<0.01). Vaccine immunized mice had a higher equivalent concentration of anti-TR specific antibodies (2324μg/ml±238μg/ml) than that of EV group (1896μg/ml±533μg/ml, p<0.01) and NS group (1736μg/ml±142μg/ml, p<0.01). Conclusions: The novel recombinant TR DNA vaccine targeting at MUC1 was exactly constructed, immunization with which could induce TR specific CTL response and antibodies response in mice. No significant financial relationships to disclose.
Yang, D, Liu, X, Jin, Y, Zhu, Y, Zeng, D, Jiang, X & Ma, H 2009, 'Electrospinning of Poly(dimethylsiloxane)/Poly(methyl methacrylate) Nanofibrous Membrane: Fabrication and Application in Protein Microarrays', Biomacromolecules, vol. 10, no. 12, pp. 3335-3340.
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Zhou, J, Zhuang, Y, Ye, S, Teng, Y, Lin, G, Zhu, B, Xie, J & Qiu, J 2009, 'Broadband downconversion based infrared quantum cutting by cooperative energy transfer from Eu2+ to Yb3+ in glasses', Applied Physics Letters, vol. 95, no. 14, pp. 141101-141101.
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Near-infrared quantum cutting involving the emission of near-infrared photons by downconversion cooperative energy transfer from Eu2+ to Yb3+ in borate glasses was reported. Electron spin resonance spectra and absorption spectra were measured to prove the existence of Eu2+ in borate glasses. Excitation, emission, and fluorescence decay measurements were performed to examine the occurrence of cooperative energy transfer from Eu2+ to Yb3+ ions. Yb3+ concentration dependent quantum efficiency was calculated and the maximum efficiency approaches 164.19%.
Jin, D, Rong, Y, Lou, W, Qin, X, Wu, W, Nin, X, Nin, X, Wang, D, Kuang, T & Qin, Y 1970, 'Therapeutic vaccination against advanced pancreatic cancer by autologous dendritic cells pulsed with a MUC1 peptide: Preclinical results of a clinical phase I trial', JOURNAL OF CLINICAL ONCOLOGY, 45th Annual Meeting of the American-Society-of-Clinical-Oncology, AMER SOC CLINICAL ONCOLOGY, FL, Orlando.
Kabakova, IV, Corcoran, B, Bolger, JA, De Sterke, CM & Eggleton, BJ 1970, 'All-optical self-switching in an optimized fiber bragg grating with a π phase shift', Optics InfoBase Conference Papers, Conference on Lasers and Electro-Optics/Quantum Electronics and Laser Science Conference (CLEO/QELS 2009), IEEE, Baltimore, MD, pp. 956-957.
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We experimentally demonstrate all-optical self-switching of sub-nanosecond pulses in a fiber grating with a π phase-shift, which acts as a cavity, enhancing the intensity. At 1.5 kW peak power the transmission increases by 4.2 dB. © 2009 Optical Society of America.
Kabakova, IV, Corcoran, B, Bolger, JA, De Sterke, CM & Eggleton, BJ 1970, 'All-optical self-switching in an optimized fiber Bragg grating with a π phase shift', 2009 Conference on Lasers and Electro-Optics and 2009 Conference on Quantum Electronics and Laser Science Conference, CLEO/QELS 2009.
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We experimentally demonstrate all-optical self-switching of sub-nanosecond pulses in a fiber grating with a π phase-shift, which acts as a cavity, enhancing the intensity. At 1.5 kW peak power the transmission increases by 4.2 dB. ©2009 Optical Society of America.
Sadraeian, M & Molaee, Z 1970, 'Bioinformatics Analyses of Deinococcus Radiodurans in Order to Waste Clean Up', 2009 Second International Conference on Environmental and Computer Science, 2009 Second International Conference on Environmental and Computer Science, IEEE.
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Wu, W, Jin, D, Lou, W, Fan, J, Wang, D & Qin, X 1970, 'A novel recombinant tandem repeat DNA vaccine targeting at MUC1', JOURNAL OF CLINICAL ONCOLOGY, 45th Annual Meeting of the American-Society-of-Clinical-Oncology, AMER SOC CLINICAL ONCOLOGY, FL, Orlando.
