Ambrosio, L, Vadalà, G, Tavakoli, J, Scaramuzzo, L, Brodano, GB, Lewis, SJ, Kato, S, Cho, SK, Yoon, ST, Kim, H-J, Gary, MF & Denaro, V 2024, 'Surgeon Preference Regarding Wound Dressing Management in Lumbar Fusion Surgery: An AO Spine Global Cross-Sectional Study', Neurospine, vol. 21, no. 1, pp. 204-211.
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Objective: To evaluate the global practice pattern of wound dressing use after lumbar fusion for degenerative conditions.Methods: A survey issued by AO Spine Knowledge Forums Deformity and Degenerative was sent out to AO Spine members. The type of postoperative dressing employed, timing of initial dressing removal, and type of subsequent dressing applied were investigated. Differences in the type of surgery and regional distribution of surgeons’ preferences were analyzed.Results: Right following surgery, 60.6% utilized a dry dressing, 23.2% a plastic occlusive dressing, 5.7% glue, 6% a combination of glue and polyester mesh, 2.6% a wound vacuum, and 1.2% other dressings. The initial dressing was removed on postoperative day 1 (11.6%), 2 (39.2%), 3 (20.3%), 4 (1.7%), 5 (4.3%), 6 (0.4%), 7 or later (12.5%), or depending on drain removal (9.9%). Following initial dressing removal, 75.9% applied a dry dressing, 17.7% a plastic occlusive dressing, and 1.3% glue, while 12.1% used no dressing. The use of no additional coverage after initial dressing removal was significantly associated with a later dressing change (p < 0.001). Significant differences emerged after comparing dressing management among different AO Spine regions (p < 0.001).Conclusion: Most spine surgeons utilized a dry or plastic occlusive dressing initially applied after surgery. The first dressing was more frequently changed during the first 3 postoperative days and replaced with the same type of dressing. While dressing policies tended not to vary according to the type of surgery, regional differences suggest that actual practice may be based on personal experience rather than available evidence.
Chen, L, Li, H, Su, Y, Yang, Z, He, Z, Wang, D, Li, JJ & Xing, D 2024, 'Using A Google Web Search Analysis to Assess the Utility of ChatGPT in Stem Cell Therapy', Stem Cells Translational Medicine, vol. 13, no. 1, pp. 60-68.
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Abstract Objective Since its introduction, the use of ChatGPT has increased significantly for medically related purposes. However, current research has not captured its applications in providing information on stem cell therapy. To address this gap, the present study compared the effectiveness of ChatGPT to Google in answering medical questions related to stem cell therapy. Methods The search term “stem cell therapy” was used to perform a Google web search, and the top 20 frequently asked questions along with answers were recorded together with relevant website sources. Of these questions, the top 10 questions were separately entered into ChatGPT, and the answers and the sources were recorded. Then, the following statement was entered into ChatGPT: “Do a Google search with the search term ‘stem cell therapy’ and record 20 common questions related to the search term.” After obtaining these questions, each question was separately entered into ChatGPT for an answer and source. Results A majority of the top 20 questions provided by Google were related to fact, whereas a majority of the questions provided by ChatGPT were related to policy. The answer sources used by Google were mostly drawn from medical practice, while those used by ChatGPT were mostly drawn from academic information. Conclusion Compared to Google, ChatGPT exhibits stronger capabilities in promoting awareness of stem cell therapy. ChatGPT has the ability to eliminate misleading informatio...
Chowdhury, S, Sais, D, Donnelly, S & Tran, N 2024, 'The knowns and unknowns of helminth–host miRNA cross-kingdom communication', Trends in Parasitology, vol. 40, no. 2, pp. 176-191.
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Crowther, CA, Ashwood, P, Middleton, PF, McPhee, A, Tran, T & Harding, JE 2024, 'Prenatal Intravenous Magnesium at 30–34 Weeks' Gestation and Neurodevelopmental Outcomes in Offspring: The MAGENTA Randomized Clinical Trial', Obstetrical & Gynecological Survey, vol. 79, no. 2, pp. 78-80.
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(Abstracted from JAMA 2023;330(7):603–614) Preterm birth is a focus of many studies and interventions but remains the leading cause of global neonatal morbidity and mortality. One particular risk that is elevated in preterm infants is cerebral palsy, which affects movement and/or posture, causing health problems and high health care costs for children and their families.
Ding, L, Chen, C, Shan, X, Liu, B, Wang, D, Du, Z, Zhao, G, Su, QP, Yang, Y, Halkon, B, Tran, TT, Liao, J, Aharonovich, I, Zhang, M, Cheng, F, Fu, L, Xu, X & Wang, F 2024, 'Optical Nonlinearity Enabled Super‐Resolved Multiplexing Microscopy', Advanced Materials, vol. 36, no. 2.
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AbstractOptical multiplexing for nanoscale object recognition is of great significance within the intricate domains of biology, medicine, anti‐counterfeiting, and microscopic imaging. Traditionally, the multiplexing dimensions of nanoscopy are limited to emission intensity, color, lifetime, and polarization. Here, a novel dimension, optical nonlinearity, is proposed for super‐resolved multiplexing microscopy. This optical nonlinearity is attributable to the energy transitions between multiple energy levels of the doped lanthanide ions in upconversion nanoparticles (UCNPs), resulting in unique optical fingerprints for UCNPs with different compositions. A vortex beam is applied to transport the optical nonlinearity onto the imaging point‐spread function (PSF), creating a robust super‐resolved multiplexing imaging strategy for differentiating UCNPs with distinctive optical nonlinearities. The composition information of the nanoparticles can be retrieved with variations of the corresponding PSF in the obtained image. Four channels multiplexing super‐resolved imaging with a single scanning, applying emission color and nonlinearity of two orthogonal imaging dimensions with a spatial resolution higher than 150 nm (1/6.5λ), are demonstrated. This work provides a new and orthogonal dimension – optical nonlinearity – to existing multiplexing dimensions, which shows great potential in bioimaging, anti‐counterfeiting, microarray assays, deep tissue multiplexing detection, and high‐density data storage.
Ding, L, Chen, C, Shan, X, Liu, B, Wang, D, Du, Z, Zhao, G, Su, QP, Yang, Y, Halkon, B, Tran, TT, Liao, J, Aharonovich, I, Zhang, M, Cheng, F, Fu, L, Xu, X & Wang, F 2024, 'Optical Nonlinearity Enabled Super‐Resolved Multiplexing Microscopy (Adv. Mater. 2/2024)', Advanced Materials, vol. 36, no. 2.
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Dios, KD, Huynh, N, Tran, TS, Center, JR & Nguyen, TV 2024, 'Association between Fat Mass and Obesity-Related Transcript Polymorphisms and Osteoporosis Phenotypes', Journal of Bone Metabolism, vol. 31, no. 1, pp. 48-55.
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Background: Common variants in the fat mass and obesity-related transcript (<i>FTO</i>) gene are related to body mass index and obesity, suggesting its potential association with bone mineral density (BMD) and fracture risk. This study sought to define the association between <i>FTO</i> gene variants and the following phenotypes: (1) BMD; (2) bone loss; and (3) fracture risk.Methods: This analysis was based on the Dubbo Osteoporosis Epidemiology Study that included 1,277 postmenopausal women aged ≥60 years living in Dubbo, Australia. BMD at the femoral neck and lumbar spine was measured biennially by dual energy X-ray absorptiometry (GE Lunar). Fractures were radiologically ascertained. Six single nucleotide polymorphisms (SNPs; rs1421085, rs1558902, rs1121980, rs17817449, rs9939609, and rs9930506) of the <i>FTO</i> gene were genotyped using TaqMan assay.Results: Women homozygous for the minor allele (GG) of rs9930506 had a significantly higher risk of hip fracture (adjusted hazard ratio, 1.93; 95% confidence interval, 1.15–3.23) than those homozygous for the major allele (AA) after adjusting for potential confounding effects. Similar associations were also observed for the minor allele of rs1121980. However, there was no significant association between the <i>FTO</i> SNPs and BMD or the rate of bone loss.Conclusions: Common variations in the <i>FTO</i> gene are associated with a hip fracture risk in women, and the association is not mediated through BMD or bone loss.
Douville, C, Lahouel, K, Kuo, A, Grant, H, Avigdor, BE, Curtis, SD, Summers, M, Cohen, JD, Wang, Y, Mattox, A, Dudley, J, Dobbyn, L, Popoli, M, Ptak, J, Nehme, N, Silliman, N, Blair, C, Romans, K, Thoburn, C, Gizzi, J, Schoen, RE, Tie, J, Gibbs, P, Ho-Pham, LT, Tran, BNH, Tran, TS, Nguyen, TV, Goggins, M, Wolfgang, CL, Wang, T-L, Shih, I-M, Lennon, AM, Hruban, RH, Bettegowda, C, Kinzler, KW, Papadopoulos, N, Vogelstein, B & Tomasetti, C 2024, 'Machine learning to detect the SINEs of cancer', Science Translational Medicine, vol. 16, no. 731.
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We previously described an approach called RealSeqS to evaluate aneuploidy in plasma cell-free DNA through the amplification of ~350,000 repeated elements with a single primer. We hypothesized that an unbiased evaluation of the large amount of sequencing data obtained with RealSeqS might reveal other differences between plasma samples from patients with and without cancer. This hypothesis was tested through the development of a machine learning approach called Alu Profile Learning Using Sequencing (A-PLUS) and its application to 7615 samples from 5178 individuals, 2073 with solid cancer and the remainder without cancer. Samples from patients with cancer and controls were prespecified into four cohorts used for model training, analyte integration, and threshold determination, validation, and reproducibility. A-PLUS alone provided a sensitivity of 40.5% across 11 different cancer types in the validation cohort, at a specificity of 98.5%. Combining A-PLUS with aneuploidy and eight common protein biomarkers detected 51% of the cancers at 98.9% specificity. We found that part of the power of A-PLUS could be ascribed to a single feature—the global reduction of AluS subfamily elements in the circulating DNA of patients with solid cancer. We confirmed this reduction through the analysis of another independent dataset obtained with a different approach (whole-genome sequencing). The evaluation of Alu elements may therefore have the potential to enhance the performance of several methods designed for the earlier detection of cancer.
Goss, DM, Vasilescu, SA, Vasilescu, PA, Cooke, S, Kim, SHK, Sacks, GP, Gardner, DK & Warkiani, ME 2024, 'Evaluation of an artificial intelligence-facilitated sperm detection tool in azoospermic samples for use in ICSI', Reproductive BioMedicine Online, pp. 103910-103910.
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Hossain, MS, Bacaoco, M, Mai, TNA, Ponchon, G, Chen, C, Ding, L, Chen, Y, Ekimov, E, Xu, X, Solntsev, AS & Tran, TT 2024, 'Fiber-Based Ratiometric Optical Thermometry with Silicon Vacancy in Microdiamonds', ACS Applied Optical Materials, vol. 2, no. 1, pp. 97-107.
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Karsa, M, Xiao, L, Ronca, E, Bongers, A, Spurling, D, Karsa, A, Cantilena, S, Mariana, A, Failes, TW, Arndt, GM, Cheung, LC, Kotecha, RS, Sutton, R, Lock, RB, Williams, O, de Boer, J, Haber, M, Norris, MD, Henderson, MJ & Somers, K 2024, 'FDA-approved disulfiram as a novel treatment for aggressive leukemia', Journal of Molecular Medicine, vol. 102, no. 4, pp. 507-519.
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Abstract Acute leukemia continues to be a major cause of death from disease worldwide and current chemotherapeutic agents are associated with significant morbidity in survivors. While better and safer treatments for acute leukemia are urgently needed, standard drug development pipelines are lengthy and drug repurposing therefore provides a promising approach. Our previous evaluation of FDA-approved drugs for their antileukemic activity identified disulfiram, used for the treatment of alcoholism, as a candidate hit compound. This study assessed the biological effects of disulfiram on leukemia cells and evaluated its potential as a treatment strategy. We found that disulfiram inhibits the viability of a diverse panel of acute lymphoblastic and myeloid leukemia cell lines (n = 16) and patient-derived xenograft cells from patients with poor outcome and treatment-resistant disease (n = 15). The drug induced oxidative stress and apoptosis in leukemia cells within hours of treatment and was able to potentiate the effects of daunorubicin, etoposide, topotecan, cytarabine, and mitoxantrone chemotherapy. Upon combining disulfiram with auranofin, a drug approved for the treatment of rheumatoid arthritis that was previously shown to exert antileukemic effects, strong and consistent synergy was observed across a diverse panel of acute leukemia cell lines, the mechanism of which was based on enhanced ROS induction. Acute leukemia cells were more sensitive to the cytotoxic activity of disulfiram than solid cancer cell lines and non-malignant cells. While disulfiram is currently under investigation in clinical trials for solid cancers, this study provides evidence for the potential of disulfiram for acute leukemia treatment. Key messages
Lai, N, Chang, G, Yang, Y, He, M, Tang, W, Huang, Q, Zhang, Q, Su, QP, Liao, J, Yang, Y, Wang, C & Wang, R 2024, 'CsPbX3 quantum Dots@ZIF-8 composites with enhanced luminescence emission and stability', Journal of Luminescence, vol. 266, pp. 120280-120280.
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Mai, TNA, Ali, S, Hossain, MS, Chen, C, Ding, L, Chen, Y, Solntsev, AS, Mou, H, Xu, X, Medhekar, N & Tran, TT 2024, 'Cryogenic Thermal Shock Effects on Optical Properties of Quantum Emitters in Hexagonal Boron Nitride', ACS Applied Materials & Interfaces, vol. 16, no. 15, pp. 19340-19349.
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Solid-state quantum emitters are vital building blocks for quantum information science and quantum technology. Among various types of solid-state emitters discovered to date, color centers in hexagonal boron nitride have garnered tremendous traction in recent years, thanks to their environmental robustness, high brightness, and room-temperature operation. Most recently, these quantum emitters have been employed for satellite-based quantum key distribution. One of the most important requirements to qualify these emitters for space-based applications is their optical stability against cryogenic thermal shock. Such an understanding has, however, remained elusive to date. Here, we report on the effects caused by such thermal shock that induces random, irreversible changes in the spectral characteristics of the quantum emitters. By employing a combination of structural characterizations and density functional calculations, we attribute the observed changes to lattice strain caused by cryogenic temperature shock. Our study sheds light on the stability of the quantum emitters under extreme conditions─similar to those countered in outer space.
Manandhar, B, Paudel, KR, Clarence, DD, De Rubis, G, Madheswaran, T, Panneerselvam, J, Zacconi, FC, Williams, KA, Pont, LG, Warkiani, ME, MacLoughlin, R, Oliver, BG, Gupta, G, Singh, SK, Chellappan, DK, Hansbro, PM & Dua, K 2024, 'Zerumbone-incorporated liquid crystalline nanoparticles inhibit proliferation and migration of non-small-cell lung cancer in vitro', Naunyn-Schmiedeberg's Archives of Pharmacology, vol. 397, no. 1, pp. 343-356.
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AbstractLung cancer is the second most prevalent type of cancer and is responsible for the highest number of cancer-related deaths worldwide. Non-small-cell lung cancer (NSCLC) makes up the majority of lung cancer cases. Zerumbone (ZER) is natural compound commonly found in the roots of Zingiber zerumbet which has recently demonstrated anti-cancer activity in both in vitro and in vivo studies. Despite their medical benefits, ZER has low aqueous solubility, poor GI absorption and oral bioavailability that hinders its effectiveness. Liquid crystalline nanoparticles (LCNs) are novel drug delivery carrier that have tuneable characteristics to enhance and ease the delivery of bioactive compounds. This study aimed to formulate ZER-loaded LCNs and investigate their effectiveness against NSCLC in vitro using A549 lung cancer cells. ZER-LCNs, prepared in the study, inhibited the proliferation and migration of A549 cells. These inhibitory effects were superior to the effects of ZER alone at a concentration 10 times lower than that of free ZER, demonstrating a potent anti-cancer activity of ZER-LCNs. The underlying mechanisms of the anti-cancer effects by ZER-LCNs were associated with the transcriptional regulation of tumor suppressor genes P53 and PTEN, and metastasis-associated gene KRT18. The protein array data showed downregulation of several proliferation associated proteins such as AXL, HER1, PGRN, and BIRC5 and metastasis-associated proteins such as DKK1, CAPG, CTSS, CTSB, CTSD, and PLAU. This study provides evidence of potential for increasing the potency and effectiveness of ZER with LCN formulation and developing ZER-LCNs as a treatment strategy for mitigation and treatment of NSCLC.
Nguyen, HG, Nguyen, HT, Nguyen, LTT, Tran, TS, Ho-Pham, LT, Ling, SH & Nguyen, TV 2024, 'Development of a shape-based algorithm for identification of asymptomatic vertebral compression fractures: A proof-of-principle study', Osteoporosis and Sarcopenia, vol. 10, no. 1, pp. 22-27.
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Razavi Bazaz, S, Sayyah, A, Hazeri, AH, Salomon, R, Abouei Mehrizi, A & Ebrahimi Warkiani, M 2024, 'Micromixer research trend of active and passive designs', Chemical Engineering Science, vol. 293, pp. 120028-120028.
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Sadeghirad, H, Yaghoubi Naei, V, O’Byrne, K, Warkiani, ME & Kulasinghe, A 2024, 'In situ characterization of the tumor microenvironment', Current Opinion in Biotechnology, vol. 86, pp. 103083-103083.
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Safarkhani, M, Farasati Far, B, Lima, EC, Jafarzadeh, S, Makvandi, P, Varma, RS, Huh, Y, Ebrahimi Warkiani, M & Rabiee, N 2024, 'Integration of MXene and Microfluidics: A Perspective', ACS Biomaterials Science & Engineering, vol. 10, no. 2, pp. 657-676.
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Salib, A, Jayatilleke, N, Seneviratne, JA, Mayoh, C, De Preter, K, Speleman, F, Cheung, BB, Carter, DR & Marshall, GM 2024, 'MYCN and SNRPD3 cooperate to maintain a balance of alternative splicing events that drives neuroblastoma progression', Oncogene, vol. 43, no. 5, pp. 363-377.
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AbstractMany of the pro-tumorigenic functions of the oncogene MYCN are attributed to its regulation of global gene expression programs. Alternative splicing is another important regulator of gene expression and has been implicated in neuroblastoma development, however, the molecular mechanisms remain unknown. We found that MYCN up-regulated the expression of the core spliceosomal protein, SNRPD3, in models of neuroblastoma initiation and progression. High mRNA expression of SNRPD3 in human neuroblastoma tissues was a strong, independent prognostic factor for poor patient outcome. Repression of SNRPD3 expression correlated with loss of colony formation in vitro and reduced tumorigenicity in vivo. The effect of SNRPD3 on cell viability was in part dependent on MYCN as an oncogenic co-factor. RNA-sequencing revealed a global increase in the number of genes being differentially spliced when MYCN was overexpressed. Surprisingly, depletion of SNRPD3 in the presence of overexpressed MYCN further increased differential splicing, particularly of cell cycle regulators, such as BIRC5 and CDK10. MYCN directly bound SNRPD3, and the protein arginine methyltransferase, PRMT5, consequently increasing SNRPD3 methylation. Indeed, the PRMT5 inhibitor, JNJ-64619178, reduced cell viability and SNRPD3 methylation in neuroblastoma cells with high SNRPD3 and MYCN expression. Our findings demonstrate a functional relationship between MYCN and SNRPD3, which maintains the fidelity of MYCN-driven alternative splicing in the narrow range required for neuroblastoma cell growth. SNRPD3 methylation and its protein-protein interface with MYCN represent novel therapeutic targets.
Stylianou, N, Sebina, I, Matigian, N, Monkman, J, Doehler, H, Röhl, J, Allenby, M, Nam, A, Pan, L, Rockstroh, A, Sadeghirad, H, Chung, K, Sobanski, T, O'Byrne, K, Almeida, ACSF, Rebutini, PZ, Machado‐Souza, C, Stonoga, ETS, Warkiani, ME, Salomon, C, Short, K, McClements, L, de Noronha, L, Huang, R, Belz, GT, Souza‐Fonseca‐Guimaraes, F, Clifton, V & Kulasinghe, A 2024, 'Whole transcriptome profiling of placental pathobiology in SARS‐CoV‐2 pregnancies identifies placental dysfunction signatures', Clinical & Translational Immunology, vol. 13, no. 2.
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AbstractObjectivesSevere Acute Respiratory Syndrome Coronavirus 2 (SARS‐CoV‐2) virus infection in pregnancy is associated with higher incidence of placental dysfunction, referred to by a few studies as a ‘preeclampsia‐like syndrome’. However, the mechanisms underpinning SARS‐CoV‐2‐induced placental malfunction are still unclear. Here, we investigated whether the transcriptional architecture of the placenta is altered in response to SARS‐CoV‐2 infection.MethodsWe utilised whole‐transcriptome, digital spatial profiling, to examine gene expression patterns in placental tissues from participants who contracted SARS‐CoV‐2 in the third trimester of their pregnancy (n = 7) and those collected prior to the start of the coronavirus disease 2019 (COVID‐19) pandemic (n = 9).ResultsThrough comprehensive spatial transcriptomic analyses of the trophoblast and villous core stromal cell subpopulations in the placenta, we identified SARS‐CoV‐2 to promote signatures associated with hypoxia and placental dysfunction. Notably, genes associated with vasodilation (NOS3), oxidative stress (GDF15, CRH) and preeclampsia (FLT1, EGFR, KISS1, PAPPA2) were enriched with SARS‐CoV‐2. Pathways related to increased nutrient uptake, vascular tension, hypertension and inflammation were also enriched in SARS‐CoV‐2 samples compared to uninfected controls.ConclusionsOur findings demonstrate the utility of spatially resolved transcriptomic analysis in defining the underlying pathogenic mechanisms of S...
Tai, T-W, Chen, H-Y, Shih, C-A, Huang, C-F, McCloskey, E, Lee, J-K, Yeap, SS, Cheung, C-L, Charatcharoenwitthaya, N, Jaisamrarn, U, Kuptniratsaikul, V, Yang, R-S, Lin, S-Y, Taguchi, A, Mori, S, Li-Yu, J, Ang, SB, Chan, D-C, Chan, WS, Ng, H, Chen, J-F, Tu, S-T, Chuang, H-H, Chang, Y-F, Chen, F-P, Tsai, K-S, Ebeling, PR, Marin, F, Nistal Rodríguez, FJ, Shi, H, Hwang, KR, Kim, K-K, Chung, Y-S, Reid, IR, Chandran, M, Ferrari, S, Lewiecki, EM, Hew, FL, Ho-Pham, LT, Nguyen, TV, Nguyen, VH, Lekamwasam, S, Pandey, D, Bhadada, S, Chen, C-H, Hwang, J-S & Wu, C-H 2024, 'Asia-Pacific consensus on long-term and sequential therapy for osteoporosis', Osteoporosis and Sarcopenia, vol. 10, no. 1, pp. 3-10.
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Welsh, JA, Goberdhan, DCI, O'Driscoll, L, Buzas, EI, Blenkiron, C, Bussolati, B, Cai, H, Di Vizio, D, Driedonks, TAP, Erdbrügger, U, Falcon‐Perez, JM, Fu, Q, Hill, AF, Lenassi, M, Lim, SK, Mahoney, MG, Mohanty, S, Möller, A, Nieuwland, R, Ochiya, T, Sahoo, S, Torrecilhas, AC, Zheng, L, Zijlstra, A, Abuelreich, S, Bagabas, R, Bergese, P, Bridges, EM, Brucale, M, Burger, D, Carney, RP, Cocucci, E, Crescitelli, R, Hanser, E, Harris, AL, Haughey, NJ, Hendrix, A, Ivanov, AR, Jovanovic‐Talisman, T, Kruh‐Garcia, NA, Ku'ulei‐Lyn Faustino, V, Kyburz, D, Lässer, C, Lennon, KM, Lötvall, J, Maddox, AL, Martens‐Uzunova, ES, Mizenko, RR, Newman, LA, Ridolfi, A, Rohde, E, Rojalin, T, Rowland, A, Saftics, A, Sandau, US, Saugstad, JA, Shekari, F, Swift, S, Ter‐Ovanesyan, D, Tosar, JP, Useckaite, Z, Valle, F, Varga, Z, van der Pol, E, van Herwijnen, MJC, Wauben, MHM, Wehman, AM, Williams, S, Zendrini, A, Zimmerman, AJ, Théry, C & Witwer, KW 2024, 'Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches', Journal of Extracellular Vesicles, vol. 13, no. 2.
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AbstractExtracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year‐on‐year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non‐vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.
Wu, C, Wan, B, Entezari, A, Fang, J, Xu, Y & Li, Q 2024, 'Machine learning-based design for additive manufacturing in biomedical engineering', International Journal of Mechanical Sciences, vol. 266, pp. 108828-108828.
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Yang, B, Sang, R, Li, Y, Goldys, E & Deng, W 2024, 'Abstract 5743: Liposome platform enables X-ray induced photodynamic therapy treatment against human triple negative breast cancer cells', Cancer Research, vol. 84, no. 6_Supplement, pp. 5743-5743.
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Abstract In this study, we employed X-ray induced photodynamic therapy (X-PDT) for the treatment on triple negative breast cancer (TNBC) cells. To do this, we rationally developed a liposome delivery system co-loaded with protoporphyrin IX (PPIX) and perfluorooctyl bromide (PFOB). Low-dose X-ray at 2Gy was employed to activate PPIX for reactive oxygen species (ROS) generation, and the co-loading of PFOB provided additional oxygen to augment ROS production. The highly toxic ROS triggered TNBC cell death. In vitro X-PDT effects including intracellular ROS generation, cytotoxicity, cell viability and apoptosis/necrosis assay in TNBC cells were studied. Our results indicate that the nanocarriers effectively induced X-PDT effect with very low dose radiation, which makes it possible to damage cancer cells. Our strategy may offer a paradigm-shifting treatment alternative for TNBC patients who need neoadjuvant radiotherapy but wish to avoid long term detrimental effect on functional outcome by undergoing X-PDT using only a fraction of the conventional radiotherapy. Citation Format: Biyao Yang, Rui Sang, Yi Li, Ewa Goldys, Wei Deng. Liposome platform enables X-ray induced photodynamic therapy treatment against human triple negative breast cancer cells [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 5743.
Yang, Y, Qi, J, Hu, J, Zhou, Y, Zheng, J, Deng, W, Inam, M, Guo, J, Xie, Y, Li, Y, Xu, C, Deng, W & Chen, W 2024, 'Lovastatin/SN38 co-loaded liposomes amplified ICB therapeutic effect via remodeling the immunologically-cold colon tumor and synergized stimulation of cGAS-STING pathway', Cancer Letters, vol. 588, pp. 216765-216765.
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