Results The expression analysis of clinical CRC samples identified 37 miRNAs that were differentially expressed between CRC and normal tissue. Furthermore, several of these miRNAs were associated with CRC tumor progression including loss of miR-133a and gain of miR-224. We identified 11 common miRNAs that were differentially expressed between normal colon and CRC in both the cell line models and clinical samples. In vitro functional studies indicated that miR-143 and miR-145 appear to function in opposing manners to either inhibit or augment cell proliferation in a metastatic CRC model. The pathways targeted by miR-143 and miR-145 showed no significant overlap. Furthermore, gene expression analysis of metastatic versus non-metastatic isogenic cell lines indicated that miR-145 targets involved in cell cycle and neuregulin pathways were significantly down-regulated in the metastatic context.
Baxter, RM, Steinbeck, MJ, Tipper, JL, Parvizi, J, Marcolongo, M & Kurtz, SM 2009, 'Comparison of periprosthetic tissue digestion methods for ultra‐high molecular weight polyethylene wear debris extraction', Journal of Biomedical Materials Research Part B: Applied Biomaterials, vol. 91B, no. 1, pp. 409-418.
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AbstractThere is considerable interest in characterization of wear debris from polyethylene (UHMWPE) bearing components used in total joint replacement. To isolate UHMWPE wear debris, tissue samples must be excised from regions adjacent to revised UHMWPE implant components, followed by exposure to one of many available tissue digestion methods. Numerous studies demonstrate successful digestion, but the relative efficiency of each method is not clear. The purpose of this study was to evaluate a variety of conditions for tissue digestion to provide a quantitative comparison of methods. Porcine and human hip tissues were exposed for 24 h to basic, acidic or enzymatic agents, filtered and digestion efficiency calculated based on the percentage of initial to final tissue weight. Of the conditions tested, 5 M NaOH, 5 M KOH, 15 M KOH or 15.8 M HNO3 yielded the most complete porcine hip tissue digestion (<1% residual tissue weight; p < 0.05). Proteinase K and Liberase Blendzyme 3 did not effectively digest tissue in a 24 h period. Similar to results from the porcine dataset, human tissues digestion was most efficient using 5 M NaOH, 5 M KOH or 15.8 M HNO3 (<1% residual tissue weight; p < 0.05). To verify that particle surface modifications did not occur after prolonged reagent exposure, GUR415 and Ceridust 3715 particles were immersed in each solution for 24 h. Overall, this study provides a framework for thorough and efficient digestive methods for UHMWPE wear debris extraction. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009
Beveridge, NJ, Tooney, PA, Carroll, AP, Tran, N & Cairns, MJ 2009, 'Down-regulation of miR-17 family expression in response to retinoic acid induced neuronal differentiation', Cellular Signalling, vol. 21, no. 12, pp. 1837-1845.
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Whole-genome microRNA and gene expression analyses were used to monitor changes during retinoic acid induced differentiation of neuroblasts in vitro. Interestingly, the entire miR-17 family was over-represented among the down-regulated miRNA. The implications of these changes are considerable, as target gene prediction suggests that the miR-17 family is involved in the regulation of the mitogen-activated protein kinase (MAPK) signaling pathway, synaptic plasticity and other markers of neuronal differentiation. Significantly, many of the target responses predicted by changes in miRNA expression were supported by the observed changes in gene expression. As expected, markers of neuronal differentiation such as anti-apoptotic protein B-cell lymphoma 2 (BCL2), myocyte enhancer factor-2D (MEF2D) and zipper protein kinase (MAP3K12; aka ZPK/MUK/DLK) were each up-regulated in response to differentiation. The expression of these genes was also reduced in response to miR-17 and miR-20a transfection, and more specifically they were also shown to contain functional miRNA recognition elements for members of the miR-17 family by reporter gene assay. This suggests that the miR-17 family have an integral role in fine-tuning the pathways involved in the regulation of neuronal differentiation. © 2009 Elsevier Inc. All rights reserved.
Biankin, AV, Kench, JG, Colvin, EK, Segara, D, Scarlett, CJ, Nguyen, NQ, Chang, DK, Morey, AL, Lee, C, Pinese, M, Kuo, SCL, Susanto, JM, Cosman, PH, Lindeman, GJ, Visvader, JE, Nguyen, TV, Merrett, ND, Warusavitarne, J, Musgrove, EA, Henshall, SM & Sutherland, RL 2009, 'Expression of S100A2 Calcium-Binding Protein Predicts Response to Pancreatectomy for Pancreatic Cancer', Gastroenterology, vol. 137, no. 2, pp. 558-568.e11.
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BACKGROUND & AIMS: Current methods of preoperative staging and predicting outcome following pancreatectomy for pancreatic cancer (PC) are inadequate. We evaluated the utility of multiple biomarkers from distinct biologic pathways as potential predictive markers of response to pancreatectomy and patient survival. METHODS: We assessed the relationship of candidate biomarkers known, or suspected, to be aberrantly expressed in PC, with disease-specific survival and response to therapy in a cohort of 601 patients. RESULTS: Of the 17 candidate biomarkers examined, only elevated expression of S100A2 was an independent predictor of survival in both the training (n = 162) and validation sets (n = 439; hazard ratio [HR], 2.19; 95% confidence interval [CI]: 1.48-3.25; P < .0001) when assessed in a multivariate model with clinical variables. Patients with high S100A2 expressing tumors had no survival benefit with pancreatectomy compared with those with locally advanced disease, whereas those without high S100A2 expression had a survival advantage of 10.6 months (19.4 vs 8.8 months, respectively) and a HR of 3.23 (95% Cl: 2.39-4.33; P < .0001). Of significance, patients with S100A2-negative tumors had a significant survival benefit from pancreatectomy even in the presence of involved surgical margins (median, 15.7 months; P = .0007) or lymph node metastases (median, 17.4 months; P = .0002). CONCLUSIONS: S100A2 expression is a good predictor of response to pancreatectomy for PC anti suggests that high S100A2 expression may be a marker of a metastatic phenotype. Prospective measurement of S100A2 expression in diagnostic biopsy samples has potential clinical Utility as a predictive marker of response to pancreatectomy and other therapies that target locoregional disease.
Bliuc, D 2009, 'Mortality Risk Associated With Low-Trauma Osteoporotic Fracture and Subsequent Fracture in Men and Women', JAMA, vol. 301, no. 5, pp. 513-513.
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Context There are few data on long-term mortality following osteoporotic fracture and fewer following subsequent fracture. Objectives To examine long-term mortality risk in women and men following all os-teoporotic fractures and to assess the association of subsequent fracture with that risk. Design, Setting, and Participants Prospective cohort from the Dubbo Osteoporosis Epidemiology Study of community-dwelling women and men aged 60 years and older from Dubbo, Australia, who sustained a fracture between April 1989 and May 2007. Main Outcome Measures Age- and sex-specific standardized mortality ratios (SMRs) compared with the overall Dubbo population for hip, vertebral, major, and minor fractures. Results In women, there were 952 low-trauma fractures followed by 461 deaths, and in men, 343 fractures were followed by 197 deaths. Age-adjusted SMRs were increased following hip fractures (SMRs, 2.43 [95% confidence interval [CI], 2.022.93] and 3.51 [95% CI, 2.65-4.66]), vertebral fractures (SMRs, 1.82 [95% CI, 1.522.17] and 2.12 [95% CI, 1.66-2.72]), major fractures (SMRs, 1.65 [95% CI, 1.312.08] and 1.70 [95% CI, 1.23-2.36]), and minor fractures (SMRs, 1.42 [95% CI, 1.19-1.70] and 1.33 [95% CI, 0.99-1.80]) for both women and men, respectively. Mortality was increased for all ages for all fractures except minor fractures for which increased mortality was only apparent for those older than 75 years. Increased mortality risk persisted for 5 years for all fractures and up to 10 years for hip fractures. Increases in absolute mortality that were above expected, for 5 years after fracture, ranged from 1.3 to 13.2 per 100 person-years in women and from 2.7 to 22.3 per 100 person-years in men, depending on fracture type. Subsequent fracture was associated with an increased mortality hazard ratio of 1.91 (95% CI, 1.54-2.37) in women and 2.99 (95% CI, 2.11-4.24) in men. Mortality risk following a subsequent fracture then declined but beyond 5 years still remained hig...
Bridge, P, Pocock, NA, Nguyen, T, Munns, C, Cowell, CT & Thompson, MW 2009, 'Prediction of Appendicular Skeletal and Fat Mass in Children: Excellent Concordance of Dual-energy X-ray Absorptiometry and Magnetic Resonance Imaging', Journal of Pediatric Endocrinology and Metabolism, vol. 22, no. 9, pp. 795-804.
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Chua, B, Gallego-Ortega, D, de Molina, A, Ullrich, A, Lacal, J & Downward, J 2009, 'Regulation of Akt(ser473) phosphorylation by Choline kinase in breast carcinoma cells', Molecular Cancer, vol. 8, no. 1, pp. 131-131.
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Cole, C, Sobala, A, Lu, C, Thatcher, SR, Bowman, A, Brown, JWS, Green, PJ, Barton, GJ & Hutvagner, G 2009, 'Filtering of deep sequencing data reveals the existence of abundant Dicer-dependent small RNAs derived from tRNAs', RNA-A PUBLICATION OF THE RNA SOCIETY, vol. 15, no. 12, pp. 2147-2160.
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Deep sequencing technologies such as Illumina, SOLiD, and 454 platforms have become very powerful tools in discovering and quantifying small RNAs in diverse organisms. Sequencing small RNA fractions always identifies RNAs derived from abundant RNA species such as rRNAs, tRNAs, snRNA, and snoRNA, and they are widely considered to be random degradation products. We carried out bioinformatic analysis of deep sequenced HeLa RNA and after quality filtering, identified highly abundant small RNA fragments, derived from mature tRNAs that are likely produced by specific processing rather than from random degradation. Moreover, we showed that the processing of small RNAs derived from tRNA Gln is dependent on Dicer in vivo and that Dicer cleaves the tRNA in vitro. Published by Cold Spring Harbor Laboratory Press. Copyright © 2009 RNA Society.
Deng, W, Drozdowicz-Tomsia, K, Jin, D & Goldys, EM 2009, 'Enhanced Flow Cytometry-Based Bead Immunoassays Using Metal Nanostructures', Analytical Chemistry, vol. 81, no. 17, pp. 7248-7255.
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Frost, SA, Nguyen, ND, Center, JR, Eisman, JA & Nguyen, TV 2009, 'Timing of Repeat BMD Measurements: Development of an Absolute Risk-Based Prognostic Model', Journal of Bone and Mineral Research, vol. 24, no. 11, pp. 1800-1807.
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AbstractThis study attempted to address the following questions: for an individual who is at present nonosteoporotic, given their current age and BMD level, what is the individual's risk of fracture and when is the ideal time to repeat a BMD measurement? Nonosteoporotic women (n = 1008) and men (n = 750) over the age of 60 in 1989 from the Dubbo Osteoporosis Epidemiology Study were monitored until one of the following outcomes occurred: (1) BMD reached “osteoporosis” level (i.e., T-scores ≤ −2.5) or (2) an incident fragility fracture. During the follow-up period (average, 7 yr), 346 women (34%) and 160 men (21%) developed osteoporosis or sustained a low-trauma fracture. The risk of osteoporosis or fracture increased with advancing age (women: RR/10 yr, 1.3; 95% CI, 1.1–1.6; men: RR/10 yr, 2.3; 95% CI, 1.7–2.9) and lower BMD levels (women: RR per −0.12 g/cm2, 3.2; 95% CI, 2.6–4.1; RR per −0.12 g/cm2, 2.6; 95% CI, 2.0–3.3). Using the predicted risk (of osteoporosis or fracture) of 10% as a cut-off level for repeating BMD measurement, the estimated time to reach the cut-off level varied from 1.5 (for an 80-yr-old woman with a T-score of −2.2) to 10.6 yr (for a 60-yr-old man with a T-score of 0). These results suggest that, based on an individual's current age and BMD T-score, it is possible to estimate the optimal time to repeat BMD testing for the individual. The prognostic model and approach presented in this study may help improve the individualization and management of osteoporosis.
Gallego-Ortega, D, Ramirez de Molina, A, Ramos, MA, Valdes-Mora, F, Barderas, MG, Sarmentero-Estrada, J & Lacal, JC 2009, 'Differential Role of Human Choline Kinase α and β Enzymes in Lipid Metabolism: Implications in Cancer Onset and Treatment', PLoS ONE, vol. 4, no. 11, pp. e7819-e7819.
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Hall, N, White, C & O’Sullivan, AJ 2009, 'The relationship between adiponectin, progesterone, and temperature across the menstrual cycle', Journal of Endocrinological Investigation, vol. 32, no. 3, pp. 279-283.
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Hasbi, A, Fan, T, Alijaniaram, M, Nguyen, T, Perreault, ML, O'Dowd, BF & George, SR 2009, 'Calcium signaling cascade links dopamine D1–D2 receptor heteromer to striatal BDNF production and neuronal growth', Proceedings of the National Academy of Sciences, vol. 106, no. 50, pp. 21377-21382.
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Although the perturbation of either the dopaminergic system or brain-derived neurotrophic factor (BDNF) levels has been linked to important neurological and neuropsychiatric disorders, there is no known signaling pathway linking these two major players. We found that the exclusive stimulation of the dopamine D1–D2 receptor heteromer, which we identified in striatal neurons and adult rat brain by using confocal FRET, led to the activation of a signaling cascade that links dopamine signaling to BDNF production and neuronal growth through a cascade of four steps: (i) mobilization of intracellular calcium through Gq, phospholipase C, and inositol trisphosphate, (ii) rapid activation of cytosolic and nuclear calcium/calmodulin-dependent kinase IIα, (iii) increased BDNF expression, and (iv) accelerated morphological maturation and differentiation of striatal neurons, marked by increased microtubule-associated protein 2 production. These effects, although robust in striatal neurons from D5−/−mice, were absent in neurons from D1−/−mice. We also demonstrated that this signaling cascade was activated in adult rat brain, although with regional specificity, being largely limited to the nucleus accumbens. This dopaminergic pathway regulating neuronal growth and maturation through BDNF may have considerable significance in disorders such as drug addiction, schizophrenia, and depression.
Ho-Pham, LT, Nguyen, ND & Nguyen, TV 2009, 'Effect of vegetarian diets on bone mineral density: a Bayesian meta-analysis', The American Journal of Clinical Nutrition, vol. 90, no. 4, pp. 943-950.
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Background: The association between vegetarian diets and bone mineral density (BMD) is controversial because of conflicting findings from previous studies. Objective: The aim of this study was to estimate the effect of vegetarian diets on BMD by using a meta-analytic approach. Design: A systematic electronic literature search was conducted to identify all relevant articles on the association between vegetarian diet and BMD. Nine studies of 2749 subjects (1880 women and 869 men) were included in the analysis. Traditional and Bayesian methods of meta-analysis were applied to synthesize the data. Results: Overall, BMD was ≈4% lower in vegetarians than in omnivores (95% CI: 2%, 7%) at both the femoral neck and the lumbar spine. Compared with omnivores, vegans had a significantly lower lumbar spine BMD (6% lower; 95% CI: 2%, 9%), which was more pronounced than in lactoovovegetarians (2% lower; 95% CI: 1%, 4%). The probability that BMD was ≥5% lower in vegetarians than in omnivores (or ≈0.3 SD) was 42% for the femoral neck and 32% for the lumbar spine. There was no evidence of publication bias. There was a moderate degree of between-study heterogeneity; the coefficient of heterogeneity varied between 46% and 51%. Conclusion: The results suggest that vegetarian diets, particularly vegan diets, are associated with lower BMD, but the magnitude of the association is clinically insignificant. © 2009 American Society for Nutrition.
Ho-Pham, LT, Nguyen, ND, Vu, BQ, Pham, HN & Nguyen, TV 2009, 'Prevalence and risk factors of radiographic vertebral fracture in postmenopausal Vietnamese women', Bone, vol. 45, no. 2, pp. 213-217.
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Ho-Pham, LT, Nguyen, PLT, Le, TTT, Doan, TAT, Tran, NT, Le, TA & Nguyen, TV 2009, 'Veganism, bone mineral density, and body composition: a study in Buddhist nuns', OSTEOPOROSIS INTERNATIONAL, vol. 20, no. 12, pp. 2087-2093.
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Lai, A, Cairns, MJ, Tran, N, Zhang, H-P, Cullen, L & Arndt, GM 2009, 'RNA Modulators of Complex Phenotypes in Mammalian Cells', PLoS ONE, vol. 4, no. 3, pp. e4758-e4758.
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Mironov, V, Zhang, J, Gentile, C, Brakke, K, Trusk, T, Jakab, K, Forgacs, G, Kasyanov, V, Visconti, RP & Markwald, RR 2009, 'Designer ‘blueprint’ for vascular trees: morphology evolution of vascular tissue constructs', Virtual and Physical Prototyping, vol. 4, no. 2, pp. 63-74.
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Organ printing is a variant of the biomedical application of rapid prototyping technology or layer-by-layer additive biofabrication of 3D tissue and organ constructs using self-assembled tissue spheroids as building blocks. Bioengineering of perfusable intraorgan branched vascular trees incorporated into 3D tissue constructs is essential for the survival of bioprinted thick 3D tissues and organs. In order to design the optimal 'blueprint' for digital bioprinting of intraorgan branched vascular trees, the coefficients of tissue retraction associated with post-printing vascular tissue spheroid fusion and remodelling must be determined and incorporated into the original CAD. Using living tissue spheroids assembled into ring-like and tube-like vascular tissue constructs, the coefficient of tissue retraction has been experimentally evaluated. It has been shown that the internal diameter of ring-like and the height of tubular-like tissue constructs are significantly reduced during tissue spheroid fusion. During the tissue fusion process, the individual tissue spheroids also change their shape from ball-like to a conus-like form. A simple formula for the calculation of the necessary number of tissue spheroids for biofabrication of ring-like structures of desirable diameter has been deduced. These data provide sufficient information to design optimal CAD for bioprinted branched vascular trees of desirable final geometry and size.
Nguyen, HTT, von Schoultz, B, Pham, DMT, Nguyen, DB, Le, QH, Nguyen, DV, Hirschberg, AL & Nguyen, TV 2009, 'Peak bone mineral density in Vietnamese women', Archives of Osteoporosis, vol. 4, no. 1-2, pp. 9-15.
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This cross-sectional study showed that peak bone mineral density in Vietnamese women is comparable to that in Caucasian women; however, the prevalence of osteoporosis in post-menopausal Vietnamese women was slightly higher than in Caucasian women. The age of achieving peak bone mass in Vietnamese women was between 26 and 30 years. Introduction: While peak bone mass and its determinants have been well-documented in Caucasian populations, little has been studied in Asian populations. The present study was designed to estimate the peak bone mineral density (BMD), age of its attainment, and to examine the prevalence of osteoporosis in Vietnamese women aged 50+. Methods: The study was designed as a cross-sectional study with 328 women aged between 10 and 65 years (average age: 41) who were randomly selected from two districts around Hanoi city according to a stratified sampling scheme. BMD at the lumbar spine, femoral neck and total hip was measured by a DXA instrument (GE Lunar Prodigy, WI, USA). BMD was modeled as a cubic function of age, from which peak BMD and age at peak BMD were estimated. Bootstrap method was utilized to estimate the 95% confidence interval of peak BMD and age at peak BMD. From the peak BMD, T-score was calculated for each woman, and using the World Health Organization criteria, any woman with femoral neck BMD T-score ≤ -2.5 was classified as having osteoporosis. Results: Peak BMD was estimated at 1.16 g/cm2 (standard deviation [SD]: 0.13 g/cm2) at the lumbar spine, 1.02 g/cm2 (SD 0.12) at the total hip, and 0.94 g/cm 2 (SD 0.11) at the femoral neck. In the cubic polynomial model, the age at peak BMD was estimated to range between 27 and 29 years. The prevalence of osteoporosis among those aged between 50 and 65 years was 23%. This prevalence was higher than in Chinese, Japanese, Korean and Caucasian populations. Conclusion: These data suggest that although the peak BMD in Vietnamese women is comparable to, the prevalence of osteoporosis is hig...
Nguyen, TV 2009, 'Calcium supplement and bone loss', Osteoporosis International, vol. 20, no. 12, pp. 2149-2149.
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Ono, M, Yamada, K, Endo, A, Avolio, F & Lamond, AI 2009, 'Analysis of Human Protein Replacement Stable Cell Lines Established using snoMEN-PR Vector', PLoS ONE, vol. 8, no. 4, pp. e62305-e62305.
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RNA-mediated gene silencing, in the form of RNA interference (RNAi) or microRNAs (miRNAs) has provided novel tools for gene discovery and validation in mammalian cells. Here, we report on the construction and application of a random small RNA expression library for use in identifying small interfering RNA (siRNA) effectors that can modify complex cellular phenotypes in mammalian cells. The library is based in a retroviral vector and uses convergent promoters to produce unique small complementary RNAs. Using this library, we identify a range of small RNA-encoding gene inserts that overcome resistance to 5-fluorouracil (5-FU)- or tumour necrosis factor alpha (TNF-a)- induced cell death in colorectal cancer cells. We demonstrate the utility of this technology platform by identifying a key RNA effector, in the form of a siRNA, which overcomes cell death induced by the chemotherapeutic 5-FU. The technology described has the potential to identify both functional RNA modulators capable of altering physiological systems and the cellular target genes altered by these modulators.
Pham, HV, Dang, DT, Tran Minh, NN, Nguyen, ND & Nguyen, TV 2009, 'Correlates of environmental factors and human plague: an ecological study in Vietnam', International Journal of Epidemiology, vol. 38, no. 6, pp. 1634-1641.
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Methods The study included all 510 communes of the Central Highlands region (with a total population of similar to 4 million) where 95% of incidence of plague cases in Vietnam had been reported from 1997 through 2002. Plague was clinically ascertained by using a standard protocol by WHO. Data on domestic fleas and rodents were obtained by using traps and periodic surveillance in accordance with the WHO guidelines. Temperature, duration of sunshine, rainfall and humidity were recorded as monthly averages by local meteorological stations. The association between these ecological factors and plague was assessed by using the Poisson regression model. Results From 1997 through 2002, 472 cases of plague were reported, of whom 24 (5.1%) died. The incidence of plague peaked during the dry season, with similar to 63% of cases occurring from February through April. The risk of plague occurrence was associated with an increased monthly flea index (RR and 95% CI: 1.93; 1.61-2.33 for months with the flea index > 1) and increased rodent density (RR 1.23; 1.15-1.32 per each 3% increase in density). Moreover, the risk of plague increased during the dry season (RR 2.07; 1.64-2.62), when rainfall fell < 10 mm (RR 1.44; 1.17-1.77). Conclusions These data suggest that the flea index, rodent density and rainfall could be used as ecological indicators of plague risk in Vietnam. The data also suggest that the occurrence of plague in Vietnam's Central Highlands is likely resulted from multiple causes that remain to be delineated.
Pham, TD, Brandl, M & Beck, D 2009, 'Fuzzy declustering-based vector quantization', Pattern Recognition, vol. 42, no. 11, pp. 2570-2577.
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Vector quantization is a useful approach for multi-dimensional data compression and pattern classification. One of the most popular techniques for vector quantization design is the LBG (Linde, Buzo, Gray) algorithm. To address the problem of producing poor estimate of vector centroids which are subjected to biased data in vector quantization; we propose a fuzzy declustering strategy for the LBG algorithm. The proposed technique calculates appropriate declustering weights to adjust the global data distribution. Using the result of fuzzy declustering-based vector quantization design, we incorporate the notion of fuzzy partition entropy into the distortion measures that can be useful for classification of spectral features. Experimental results obtained from simulated and real data sets demonstrate the effective performance of the proposed approach. © 2009 Elsevier Ltd. All rights reserved.
Sabir, MI, Xu, X & Li, L 2009, 'A review on biodegradable polymeric materials for bone tissue engineering applications', Journal of Materials Science, vol. 44, no. 21, pp. 5713-5724.
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Siddiquee, N, Parween, S, Quddus, M & Barua, P 2009, 'Heavy Metal Pollution in Sediments at Ship Breaking Area of Bangladesh', ASIAN JOURNAL OF WATER ENVIRONMENT AND POLLUTION, vol. 6, no. 3, pp. 7-12.
Styrkarsdottir, U, Halldorsson, BV, Gretarsdottir, S, Gudbjartsson, DF, Walters, GB, Ingvarsson, T, Jonsdottir, T, Saemundsdottir, J, Snorradóttir, S, Center, JR, Nguyen, TV, Alexandersen, P, Gulcher, JR, Eisman, JA, Christiansen, C, Sigurdsson, G, Kong, A, Thorsteinsdottir, U & Stefansson, K 2009, 'New sequence variants associated with bone mineral density', Nature Genetics, vol. 41, no. 1, pp. 15-17.
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Subramony, SH, Nguyen, TV, Langford, L, Lin, X, Parent, AD & Zhang, J 2009, 'Identification of a new form of autosomal dominant spastic paraplegia', Clinical Genetics, vol. 76, no. 1, pp. 113-116.
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Teymourtash, AR & Ebrahimi Warkiani, M 2009, 'Natural convection over a non-isothermal vertical flat plate in supercritical fluids', Scientia Iranica, vol. 16, no. 6 B, pp. 470-478.
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In many applications, convection heat transfer is coupled with conduction and radiation heat transfer, which generate temperature gradients along the walls and may greatly affect natural convection heat transfer. The main objective of this study is to calculate the heat-transfer characteristics for natural convection from a non-isothermal vertical flat plate into a supercritical fluid. The influence of the non-uniformity of wall temperature on the heat transfer by natural convection along a vertical plate, having a linearly distributed temperature (characterized by the slope S) is also investigated. The thermal expansion coefficient is considered as a function of the temperature, the pressure, the van der Waals constants and the compressibility factor. The trends of the curves obtained with this equation and with values from tables of thermodynamic properties were similar and diverged at a critical point. These features confirmed the validity of this equation. Then, the governing systems of partial differential equations are solved numerically using the finite difference method. The local Nusselt number was then calculated and plotted as a function of the local Rayleigh number. It was observed that a positive slope of temperature distribution increases the heat transfer rate and a negative slope decreases it. © Sharif University of Technology, December 2009.
Teymourtash, AR & Warkiani, ME 2009, 'Natural Convection over a Non-Isothermal Vertical Flat Plate in Supercritical Fluids', SCIENTIA IRANICA TRANSACTION B-MECHANICAL ENGINEERING, vol. 16, no. 6, pp. 470-478.
Tran, BNH, Nguyen, ND, Center, JR, Eisman, JA & Nguyen, TV 2009, 'Enhancement of Absolute Fracture Risk Prognosis with Genetic Marker: The Collagen I Alpha 1 Gene', Calcified Tissue International, vol. 85, no. 5, pp. 379-388.
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Tran, N 2009, 'Fast and Simple microRNA Northern Blots', Biochemistry Insights, vol. 2, pp. BCI.S2257-BCI.S2257.
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RNA northern blots provide robust measurements of gene expression. The simple northern blot technique described in this report has been optimised to provide rapid, reproducible detection and analysis of mature and precursor forms of microRNAs. This protocol economises on the use of commercially available components and secondly reduces the hybridisation step to 2 hours.
Tran, N 2009, 'Fast and Simple micro-RNA Northern Blots', BIOCHEMISTRY INSIGHTS, vol. 2, pp. 1-3.
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RNA northern blots provide robust measurements of gene expression. The simple northern blot technique described in this report has been optimised to provide rapid, reproducible detection and analysis of mature and precursor forms of microRNAs. This protocol economises on the use of commercially available components and secondly reduces the hybridisation step to 2 hours.
Valdés-Mora, F, Gómez del Pulgar, T, Bandrés, E, Cejas, P, Ramírez de Molina, A, Pérez-Palacios, R, Gallego-Ortega, D, García-Cabezas, MA, Casado, E, Larrauri, J, Nistal, M, González-Barón, M, García-Foncillas, J & Lacal, JC 2009, 'TWIST1 Overexpression is Associated with Nodal Invasion and Male Sex in Primary Colorectal Cancer', Annals of Surgical Oncology, vol. 16, no. 1, pp. 78-87.
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Van Landingham, M, Nguyen, TV, Roberts, A, Parent, AD & Zhang, J 2009, 'Risk factors of congenital hydrocephalus: a 10 year retrospective study', Journal of Neurology, Neurosurgery & Psychiatry, vol. 80, no. 2, pp. 213-217.
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White, CP 2009, 'Calcium metabolism in pregnancy and lactation', Obstetric Medicine, vol. 2, no. 1, pp. 2-5.
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Homeostatic adaptation to maternal calcium metabolism is a prerequisite for optimal delivery of sufficient calcium to the fetus and neonate during pregnancy and lactation, respectively. This article outlines the major adaptations known to occur and the physiological regulators likely to be principally involved. Importantly, different adaptive responses are used in pregnancy and lactation. The rarity of calcium disorders in pregnancy underscores the successful implementation of these adaptations in most women. For those few women with either pre-existing or pregnancy-acquired disorders of calcium metabolism, a knowledge of normal physiology is essential to understand the implications for managing these disorders in pregnant women.
Wirth, MA, Klotz, C, Deffenbaugh, DL, McNulty, D, Richards, L & Tipper, JL 2009, 'Cross-linked glenoid prosthesis: A wear comparison to conventional glenoid prosthesis with wear particulate analysis', Journal of Shoulder and Elbow Surgery, vol. 18, no. 1, pp. 130-137.
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Wong, MHW, Dobbins, TA, Tseung, J, Tran, N, Lee, CS, O’Brien, CJ, Clark, J & Rose, BR 2009, 'Oestrogen receptor β expression in pleomorphic adenomas of the parotid gland', Journal of Clinical Pathology, vol. 62, no. 9, pp. 789-793.
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Aims:Pleomorphic adenomas of the salivary gland have gender and age distributions suggesting that oestrogen has a causal role. However, oestrogen receptor (ER)α is expressed at low levels in normal salivary gland tissues and data from salivary gland tumours are conflicting. There is preliminary evidence that the recently described ERβ may be the major ER in salivary gland tissue. The aim of this study was to determine the nature and extent of ERβ expression in pleomorphic adenomas of the salivary gland.Methods:Pleomorphic adenomas and normal tissues of the parotid gland from 49 patients were tested for ERα and ERβ expression by semiquantitative immunohistochemistry. Associations were sought with patient age and gender.Results:ERα and ERβ expression was localised mainly to the nuclei of ductal cells in normal tissues and the epithelial components in pleomorphic adenomas. Within each tissue and receptor type there were no associations between ER positivity and patient age or gender. ERβ was expressed in almost twice as many normal tissues and pleomorphic adenomas as ERα. Expression of ERβ was also significantly higher in tumour compared with normal tissues.Conclusions:This is thought to be the first study of ERβ in pleomorphic adenomas of the salivary gland. Findings support ERβ as the major ER in salivary glands, and provide evidence that ERβ may have a role in the development of pleomorphic adenomas of the salivary gland.
Yang, D, Liu, X, Jin, Y, Zhu, Y, Zeng, D, Jiang, X & Ma, H 2009, 'Electrospinning of Poly(dimethylsiloxane)/Poly(methyl methacrylate) Nanofibrous Membrane: Fabrication and Application in Protein Microarrays', Biomacromolecules, vol. 10, no. 12, pp. 3335-3340.
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Yang, Z, Ding, G, Cai, H, Xu, X, Wang, H & Zhao, X 2009, 'Analysis and elimination of the ‘skip contact’ phenomenon in an inertial micro-switch for prolonging its contact time', Journal of Micromechanics and Microengineering, vol. 19, no. 4, pp. 045017-045017.
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Yuan, C, Jin, Z, Tipper, JL & Yan, X 2009, 'Numerical surface characterization of wear debris from artificial joints using atomic force microscopy', Science Bulletin, vol. 54, no. 24, pp. 4583-4588.
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Zhang, X, Cairns, M, Rose, B, O'Brien, C, Shannon, K, Clark, J, Gamble, J & Tran, N 2009, 'Alterations in miRNA processing and expression in pleomorphic adenomas of the salivary gland', International Journal of Cancer, vol. 124, no. 12, pp. 2855-2863.
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AbstractGenome‐wide microRNA (miRNA) expression profiling of salivary gland pleomorphic adenomas revealed a distinct expression signature consisting largely of upregulated miRNAs compared with matched normal tissue. Microarray data were confirmed by quantitative real time RT‐PCR (q‐RTPCR). Five miRNA genes upregulated in the tumours were found in close proximity to fragile sites and/or cancer associated genomic regions. Interestingly, q‐RTPCR revealed an increase in the expression of components of the miRNA processing machinery (Dicer, Drosha, DGCR8 and p68) in tumours suggesting that the deregulation of miRNA expression may result from increased miRNA biogenesis. Target gene prediction analysis of the altered miRNAs indicated that genes in a number of signalling pathways important in tumourigenesis including WNT, MAPK and JAK‐STAT were overrepresented. Significantly, the oncogene PLAG1 was overexpressed in our cohort and may be potentially regulated by these miRNAs. This is the first study to examine changes in the miRNA milieu in pleomorphic adenoma, the most common salivary gland tumour. This study has demonstrated an upregulation of both miRNAs genes and an upregulation of the miRNA processing machinery. These changes may be potential underlying mechanisms for the development of these benign tumours. © 2009 UICC
Zhao, HY, Zheng, W, Meng, ZX, Zhou, HM, Xu, XX, Li, Z & Zheng, YF 2009, 'Bioelectrochemistry of hemoglobin immobilized on a sodium alginate-multiwall carbon nanotubes composite film', Biosensors and Bioelectronics, vol. 24, no. 8, pp. 2352-2357.
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