Braun, M & Wilson, BC 1982, 'Comparative evaluation of several rare-earth film-screen systems.', Radiology, vol. 144, no. 4, pp. 915-919.
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Comparisons of image quality were made for several rare-earth film-screen combinations under conditions similar to those used in chest radiography. The characteristic curve, modulation transfer function, and Wiener spectrum were measured. Observer response studies were carried out to compare the limits of perceived resolution. Signal-to-noise considerations based on the measured characteristics favor a fast film-slow screen pair over a slow film-fast screen pair, consistent with the observer study results. The interrelationship of the physical parameters in determining image quality and the limitations of the method are discussed.
Coombe, RG & George, AM 1982, 'Purification and properties of an aminoglycoside acetyltransferase from Pseudomonas aeruginosa', Biochemistry, vol. 21, no. 5, pp. 871-875.
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An aminoglycoside 3-acetyltransferase [AAC(3)], possibly a new isoenzymic species of the 3-N-acetyltransferase group, was purified to apparent homogeneity from a crude extract of Pseudomonas aeruginosa, a gentamicin-resistant clinical isolate. The method of purification was consecutive column chromatography—(i) gel filtration, (ii) affinity chromatography, and (iii) ion-exchange chromatography—to give two protein peaks, one of which was coincident with activity and which indicated a purification of 600 (specific activity = 9.743 units mg−1 at pH 7.2, 34 °C). Polyacrylamide disc gel electrophoresis indicated a single protein band coincident with enzymic activity. The molecular weight of the enzyme was about 39 000. AAC(3)-V (provisonal designation) was further characterized by stability, substrate, pH, and kinetic studies. The Km was 0.724 µM (sisomicin), and the Vmax was 0.102 µmol min−1 mg−1 (sisomicin) at pH 7.2 and 34 °C. Substrate inhibition was exhibited by kanamycin A and tobramycin. Studies showed that enzyme activity was significantly stabilized when preparations contained substrate. © 1982, American Chemical Society. All rights reserved.
FLICKER, W, MILTHORPE, BK, SCHINDHELM, K, ODELL, RA, MCPHERSON, J & FARRELL, PC 1982, 'PLATELET FACTOR RELEASE FOLLOWING HEPARIN ADMINISTRATION AND DURING EXTRACORPOREAL-CIRCULATION', TRANSACTIONS AMERICAN SOCIETY FOR ARTIFICIAL INTERNAL ORGANS, vol. 28, pp. 431-436.
