Belli, SI, Mercuri, F, Sali, A & Goding, J 1995, 'Autophosphorylation Of Pc-1 (alkaline Phosphodiesterase-i Nucleotide Pyrophosphatase) And Analysis Of The Active-site', European Journal Of Biochemistry, vol. 228, no. 3, pp. 669-676.
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PC-1 is an ecto-enzyme possessing alkaline phosphodiesterase I (EC 3.1.4.1) and nucleotide pyrophosphatase (EC 3.6.1.9) activities. It has also been proposed to be an ecto-protein kinase capable of phosphorylating itself as well as exogenous proteins. We
Belli, SI, Mercuri, FA, Sali, A & Goding, JW 1995, 'Autophosphorylation of PC-1 (Alkaline Phosphodiesterase I/Nucleotide Pyrophosphatase) and Analysis of the Active Site', European Journal of Biochemistry, vol. 228, no. 3, pp. 669-676.
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Belli, SI, Mercuri, FA, Sali, A & Goding, JW 1995, 'Autophosphorylation of PC‐1 (Alkaline Phosphodiesterase I/Nucleotide Pyrophosphatase) and Analysis of the Active Site', European Journal of Biochemistry, vol. 228, no. 3, pp. 669-676.
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PC‐1 is an ecto‐enzyme possessing alkaline phosphodiesterase I (EC 3.1.4.1) and nucleotide pyrophosphatase (EC 3.6.1.9) activities. It has also been proposed to be an ecto‐protein kinase capable of phosphorylating itself as well as exogenous proteins. We have investigated the phosphorylation capability of PC‐1 and have developed a novel method for its detection and characterization based on autophosphorylation, which allows detection without the use of antibodies. When cells expressing membrane PC‐1 were held on ice with [γ‐32P]ATP, SDS/PAGE of whole cell lysates showed a single band which was PC‐1; this band was absent in cells not expressing PC‐1. Immunoprecipitates of soluble PC‐1 isolated from culture supernatants of cells expressing PC‐1 were also capable of autophosphorylation, and the size of the labeled protein was the same as previously reported for soluble PC‐1. PC‐1 was also labeled with [γ‐32P]ATP and [α‐35S]dATP[αS]. We found no evidence that PC‐1 was capable of phosphorylating proteins other than itself, and conclude that it is not a true kinase, and that the observed labeling with [γ‐32P]ATP, [α‐32P]ATP and [35S]dATP[αS] reflect transient covalent adducts that are part of the catalytic cycle of phosphodiesterase/pyrophosphatase activity rather than intrinsic kinase activity. Mutation of the active‐site threonine to tyrosine, serine or alanine reduced the 5′‐nucleotide phosphodiesterase activity of PC‐1 and its ability to autophosphorylate to undetectable levels. Together, these data suggest that both activities depend on the same site. Copyright © 1995, Wiley Blackwell. All rights reserved
Doblin, MA & Clayton, MN 1995, 'Effects Of Secondarily Treated Sewage Effluent On The Early Life-History Stages Of 2 Species Of Brown Macroalgae - Hormosira-Banksii And Durvillaea-Potatorum', Marine Biology, vol. 122, no. 4, pp. 689-698.
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Sewage effluent has a deleterious effect on the early life-history stages of Hosmosira banksii (Turner) Decaisne and Durvillaea potatorum (Labillardiere) Areschoug. High concentrations of sewage effluent (28 and 40% in seawater) inhibit zygote germinatio
Field, CD 1995, 'Impact of expected climate change on mangroves', Hydrobiologia, vol. 295, no. 1-3, pp. 75-81.
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There is a consensus of scientific opinion that the activities of man will cause a significant change in the global climate over the next hundred years. The rising level of carbon dioxide and other industrial gases in the atmosphere may lead to global warming with an accompanying rise in sea-level. Mangrove ecosystems grow in the intertidal zones in tropical and sub-tropical regions and are likely to be early indicators of the effects of climate change. The best estimates of predicted climate change in the literature are presented. It is suggested that a rise in mean sea-level may be the most important factor influencing the future distribution of mangroves but that the effect will vary dramatically depending on the local rate of sea-level rise and the availability of sediment to support reestablishment of the mangroves. The predicted rise in mean air temperature will probably be of little consequence to the development of mangroves in general but it may mean that the presence of mangroves will move further north and south, though this will depend on a number of additional factors. The effect of enhanced atmospheric CO2 on the growth of mangroves is unknown at this time but that there is some evidence that not all species of mangroves will respond similarly. The socio-economic impacts of the effects of climate on mangrove ecosystems may include increased risk of flooding, increased erosion of coast lines, saline intrusion and increased storm surges. © 1995 Kluwer Academic Publishers.
Klimant, I, Meyer, V & Kuhl, M 1995, 'Fiber-optic oxygen microsensors, a new tool in aquatic biology', Limnology and Oceanography, vol. 40, no. 6, pp. 1159-1165.
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Kuhl, M, Cohen, Y, Dalsgaard, T, Jorgensen, BB & Revsbech, NP 1995, 'Microenvironment and photosynthesis of zooxanthellae in scleractinian corals studied with microsensors for O2, pH and light', Marine Ecology Progress Series, vol. 117, pp. 159-172.
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Larkum, AWD 1995, 'Halophila capricorni (Hydrocharitaceae): a new species of seagrass from the Coral Sea', Aquatic Botany, vol. 51, no. 3-4, pp. 319-328.
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A new seagrass species, Halophila capricorni Larkum, is described in the section Halophila. This species differs from Halophila decipiens Ostenfeld in (i) its generally larger size, (ii) the pattern of large hairs on the proximal region of the abaxial leaf surface, (iii) the presence of male and female flowers in separate axils of the same plant, (iv) styles 15-25 mm long, and (v) dispersal by floating fruits. Halophila capricorni is found on sand in deep water in coral environments on the Great Barrier Reef and in New Caledonia. It may also be present in the Philippines and adjoining regions. © 1995.
O'Meara, TJ, Nesa, M, Seaton, DS & Sandeman, RM 1995, 'A comparison of inflammatory exudates released from myiasis wounds on sheep bred for resistance or susceptibility to Lucilia cuprina', Veterinary Parasitology, vol. 56, no. 1-3, pp. 207-223.
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Oren, A, Kuhl, M & Karsten, U 1995, 'An engoevaporitic microbial mat within a gypsum crust: zonation of phototrophs, photopigments, and light penetration', Marine Ecology Progress Series, vol. 128, pp. 151-159.
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Ralph, PJ & Burchett, M 1995, 'Photosynthetic Responses Of The Seagrass Halophila-Ovalis (R Br) Hook F To High Irradiance Stress, Using Chlorophyll-A Fluorescence', Aquatic Botany, vol. 51, no. 1-Feb, pp. 55-66.
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With the increasing threat of destruction to diminishing seagrass resources, there is a need for an accurate stress monitoring procedure to be developed for species of this community. In this study chlorophyll a fluorescence (20 degrees C) was used to mo
HOLST, G, KUHL, M & KLIMANT, I 1995, 'A novel measuring system for oxygen microoptodes based on a phase modulation technique', CHEMICAL, BIOCHEMICAL, AND ENVIRONMENTAL FIBER SENSORS VII, Conference on Chemical, Biochemical and Environmental Fiber Sensors VII, SPIE - INT SOC OPTICAL ENGINEERING, MUNICH, GERMANY, pp. 387-398.
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KLIMANT, I, HOLST, G & KUHL, M 1995, 'Oxygen microoptrodes and their application in aquatic environment', CHEMICAL, BIOCHEMICAL, AND ENVIRONMENTAL FIBER SENSORS VII, Conference on Chemical, Biochemical and Environmental Fiber Sensors VII, SPIE - INT SOC OPTICAL ENGINEERING, MUNICH, GERMANY, pp. 375-386.
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