., AVR, ., KPU, ., KMH, ., IB & ., KVM 2007, 'Studies on Morphological and Phytochemical Variability of Different Populations of Tribulus terrestris', International Journal of Plant Breeding and Genetics, vol. 1, no. 2, pp. 95-100.
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Andrews, M, Raven, JA, Sprent, JI & Lea, PJ 2007, 'Is shoot growth correlated to leaf protein concentration?', TRENDS IN PLANT SCIENCE, vol. 12, no. 12, pp. 531-532.
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Andrews, M, Scholefield, D, Abberton, MT, McKenzie, BA, Hodge, S & Raven, JA 2007, 'Use of white clover as an alternative to nitrogen fertiliser for dairy pastures in nitrate vulnerable zones in the UK: Productivity, environmental impact and economic considerations', Annals of Applied Biology, vol. 151, no. 1, pp. 11-23.
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Perennial ryegrass and perennial ryegrass/white clover permanent dairy pastures are compared with respect to productivity, environmental impact and financial costs in nitrate vulnerable zones (NVZ) in the UK. With appropriate management, and utilisation of recommended perennial ryegrass and white clover cultivars, white clover is likely to stabilise at around 20% of total dry matter production in a mixed pasture. Plant dry matter production and milk production from a perennial ryegrass/white clover pasture are likely to be similar to that from a perennial ryegrass pasture receiving 200 kg N ha-1 annum -1 and around 70% of that obtained with perennial ryegrass supplied with 350-400 kg N ha-1 annum-1. Nitrate, phosphorus and methane losses from the system and decreases in biodiversity relative to a grazed indigenous sward are likely to be similar for a perennial ryegrass/white clover pasture and a perennial ryegrass pasture receiving 200 kg N ha -1 annum-1: nitrate leachate from both systems is likely to comply with European legislation. Greenhouse gas emissions resulting from nitrogen (N) fertiliser production would be avoided with the perennial ryegrass/white clover pasture. Within NVZ stocking rate restrictions, white clover can provide the N required by a pasture at a lower financial cost than that incurred by the application of N fertiliser. © 2007 Association of Applied Biologists.
Andrews, M, Scholefield, D, Abberton, MT, McKenzie, BA, Hodge, S & Raven, JA 2007, 'Use of white clover as an alternative to nitrogen fertiliser for dairy pastures in nitrate vulnerable zones in the UK: productivity, environmental impact and economic considerations', ANNALS OF APPLIED BIOLOGY, vol. 151, no. 1, pp. 11-23.
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Bateman, RL, Ashworth, J, Witte, JF, Baker, L-J, Bhanumoorthy, P, Timm, DE, Hurley, TD, Grompe, M & McClard, RW 2007, 'Slow-onset inhibition of fumarylacetoacetate hydrolase by phosphinate mimics of the tetrahedral intermediate: kinetics, crystal structure and pharmacokinetics', BIOCHEMICAL JOURNAL, vol. 402, pp. 251-260.
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Beerling, DJ, Hewitt, CN, Pyle, JA & Raven, JA 2007, 'Critical issues in trace gas biogeochemistry and global change', PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES, vol. 365, no. 1856, pp. 1629-1642.
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Bishop, M, Kelaher, BP, Alquezar, R, York, PH, Ralph, PJ & Skilbeck, G 2007, 'Trophic cul-de-sac, Pyrazus ebeninus, limits trophic transfer through an estuarine detritus-based food web', OIKOS, vol. 116, no. 3, pp. 427-438.
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The importance to food-webs of trophic cul-de-sacs, species that channel energy flow away from higher trophic levels, is seldom considered outside of the pelagic systems in which they were first identified. On intertidal mudflats, inputs of detritus from saltmarshes, macroalgae or microphytobenthos are generally regarded as a major structuring force underpinning food-webs and there has been no consideration of trophic cul-de-sacs to date. A fully orthogonal three-factor experiment manipulating the density of the abundant gastropod, Pyrazus ebeninus, detritus and macrobenthic predators on a Sydney mudflat revealed large deleterious effects of the gastropod, irrespective of detrital loading or the presence of predators. Two months after experimental manipulation, the standing-stock of microphytobenthos in plots with high (44 per m(2)) densities of P. ebeninus was 20% less than in plots with low (4 per m(2)) densities. Increasing densities of P. ebeninus from low to high halved the abundance of macroinvertebrates and the average number of species. In contrast, the addition of detritus had differing effects on microphytobenthos (positively affected) and macroinvertebrates (negatively affected). Over the two-months of our experiment, no predatory mortality of P. ebeninus was observed and high densities of P. ebeninus decreased impacts of predators on macroinvertebrate abundances. Given that the dynamics of southeast Australian mudflats are driven more by disturbance than seasonality in predators and their interactions with prey, it is likely that Pyrazus would be similarly resistant to predation and have negative effects on benthic assemblages at other times of the year, outside of our study period. Thus, in reducing microphytobenthos and the abundance and species richness of macrofauna, high abundances of the detritivore P. ebeninus may severely limit the flow of energy up the food chain to commercially-important species.
Bostrom, KH, Riemann, L, Kuhl, M & Hagstrom, A 2007, 'Isolation and gene quantification of heterotrophic N2-fixing bacterioplankton in the Baltic Sea', Environmental Microbiology, vol. 9, no. 1, pp. 152-164.
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Cyanobacteria are regarded as the main N2-fixing organisms in marine waters. However, recent clone libraries from various oceans show a wide distribution of the dinitrogenase reductase gene (nifH) originating from heterotrophic bacterioplankton. We isolated heterotrophic N2-fixing bacteria from Baltic Sea bacterioplankton using low-nitrogen plates and semi-solid diazotroph medium (SSDM) tubes. Isolates were analysed for the nitrogenase (nifH) gene and active N2 fixation by nested polymerase chain reaction (PCR) and acetylene reduction respectively. A primer-probe set targeting the nifH gene from a ?-proteobacterial isolate, 97% 16S rDNA similarity to Pseudomonas stutzeri, was designed for measuring in situ dynamics using quantitative real-time PCR. This nifH gene sequence was detected at two of 11 stations in a Baltic Proper transect at abundances of 3 × 104 and 0.8 × 103 copies per litre seawater respectively. Oxygen requirements of isolates were examined by cultivation in SSDM tubes where oxygen gradients were determined with microelectrodes. Growth, and thereby N2 fixation, was observed as horizontal bands formed at oxygen levels of 06% air saturation. The apparent microaerophilic or facultative anaerobic nature of the isolates explains why the SSDM approach is the most appropriate isolation method. Our study illustrates how combined isolation, functional analyses and in situ quantification yielded insights into the oxygen requirements of heterotrophic N2-fixing bacterioplankton isolates, which were confirmed to be present in situ.
Cockell, CS & Raven, JA 2007, 'Ozone and life on the Archaean earth', PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY A-MATHEMATICAL PHYSICAL AND ENGINEERING SCIENCES, vol. 365, no. 1856, pp. 1889-1901.
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Collier, C, Lavery, P, Masini, R & Ralph, PJ 2007, 'Morphological, growth and meadow characteristics of the seagrass Posidonia sinuosa along a depth-related gradient of light availability', Marine Ecology: Progress Series, vol. 337, pp. 103-115.
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Morphological and growth characteristics of the meadow-forming seagrass Posidonia sinuosa (Cambridge et Kuo), were measured along a depth-related gradient of light to infer its response to long-term differences in light availability. Morphometric measurements were carried out at 6 depths between 1.6 and 9.0 m in summer and winter at Cockburn Sound and summer only at Warnbro Sound in south-western Australia. The minimum light requirement for P. sinuosa of 8.5% sub-surface light was among the lower range reported for seagrasses. Its slow growth rate (0.5?1.5 mgdry shoot?1 d?1), relative to similarly sized species, may contribute to the low light requirements of this species. Shoot density, leaf area index and biomass showed pronounced and consistent differences among depths (up to 88-fold reduction of above-ground biomass from shallow to deep sites). At the deeper sites, the reduced shoot density probably reduces respiratory demand and alleviates self-shading. Morphological differences (leaf length, width and thickness and number of leaves per shoot) did not follow a clear and consistent trend with depth. Despite a 70% reduction in light availability at the canopy level between the shallowest and deepest sites, leaf growth rate was unaffected by depth during summer, and in winter differed between only a few depths. We propose that the reduction in shoot density partially alleviates the effects of self-shading and permits comparable leaf growth rates across the depth range. These results suggest that for interpreting long-term responses to light availability, shoot density is the most sensitive of the morphological characteristics measured here.
Doblin, MA, Coyne, K, Rinta-kanto, J, Wilhelm, S & Dobbs, FC 2007, 'Dynamics and short-term survival of toxic cyanobacteria species in ballast water from NOBOB vessels transiting the Great Lakes - implications for HAB invasions', Harmful Algae, vol. 6, no. 4, pp. 519-530.
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We measured the presence, viability and potential toxicity of cyanobacteria in ships' ballast tanks during three domestic voyages through the North American Great Lakes. Using molecular methods, the toxin-producing forms of Microcystis and Anabaena were monitored in ballast water after ships' ballast tanks were filled at their first port of call, and at subsequent ports as ships transited the Great Lakes. Microcystis weas detected in ballast water at intermidiate and final ports of call in all three experiemnts, but the presence of Anabaena was more variable, suggesting low abundance or patchy distribution in ballast tanks. Both species were detected in ballast water up to 11 days old. Detection of the mucrocystin synthetase gene, mcyE, in ballst tanks indicated entrained cells were capable of producing mycrocystin, and further analyses of RNA indicated the toxin was being expressed by Microcystis, even after 11 days in dark transit. These data demonstrate within-basin transport and delivery of planktonic harmful algal bllom (HAB) species to distant ports in the world's largest freshwater resevoir, with potential implications for drinking water quality. These implications are discussed with respect to management of microbial invasions and the fate of introduced phytoplankton in their receiving environment.
Drake, L, Doblin, MA & Dobbs, FC 2007, 'Potential microbial bioinvasions via ships' ballast water, sediment, and biofilm', Marine Pollution Bulletin, vol. 55, no. 7-9, pp. 333-341.
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A prominent vector of aquatic invasive species to coastal regions is the discharge of water, sediments and biofilm from ships' ballast-water tanks. During eight years of studying ships arriving to the lower Chesapeake Bay, we developed an undertsnading of th mechanisms by which invasive microorganisms might arrive to the region via ships. Within a given ship, habitats included ballast water, unpumpable water and sediment (collectively known as residuals) and biofilms formed on internal surfaces of ballast-water tanks. We sampled 69 vessels arriving from foreign and domestic ports, largely from Western Europe and Mediterranean region and the US East and Gulf coasts. All habitats contained bacteria and viruses. By extrapolating the measured concentration of a microbial metric to the estimated volume of ballast water, biofilm or residual sediment and water within an average vessel, we calculated the potential total number of microorganisms contained by each habitat, thus creating a hierarchy of risk of delivery. The estimated concentration of microorganisms was greatest in ballast water >> sediment and water residuals >> biofilms. From these results, it is clear microorganisms may be transported within ships ina varierty of ways. Using temperature tolerence as a measure of survivability and the temperature difference between ballst-water samples and the water into which the ballast water was discharged, we estimated 56% of microorganisms could survive in the lower Bay. Extrapolated delivery and survival of microorganisms to thePort of Hampton Roads in lower Chesapeake Bay shows on the order of 10 20 microorganisms are discharged annually into the region.
Duke, NC, Meynecke, JO, Dittman, S, Ellison, AM, Anger, K, Berger, U, Cannicci, S, Diele, K, Ewel, KC, Field, C, Koedam, N, Lee, SY, Marchand, C, Nordhaus, I & Dahdouh-Guebas, F 2007, 'A world without mangroves?', Science, vol. 317, no. 5834, pp. 41-42.
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At a meeting of world mangrove experts held last year in Australia, it was unanimously agreed that we face the prospect of a world deprived of the services offered by mangrove ecosystems, perhaps within the next 100 years. Mangrove forests once covered more than 200,000 km2 of sheltered tropical and subtropical coastlines (1). They are disappearing worldwide by 1 to 2% per year, a rate greater than or equal to declines in adjacent coral reefs or tropical rainforests (2-5). Losses are occurring in almost every country that has mangroves, and rates continue to rise more rapidly in developing countries, where >90% of the world's mangroves are located. The veracity and detail of the UN Food and Agriculture Organization data (2) on which these observations are based may be arguable, but mangrove losses during the last quarter century range consistently between 35 and 86%. As mangrove areas are becoming smaller or fragmented, their long-term survival is at great risk, and essential ecosystem services may be lost.
Eastberg, JH, Smith, AM, Zhao, L, Ashworth, J, Shen, BW & Stoddard, BL 2007, 'Thermodynamics of DNA target site recognition by homing endonucleases', NUCLEIC ACIDS RESEARCH, vol. 35, no. 21, pp. 7209-7221.
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Finkl, CW & Restrepo Coupe, N 2007, 'Potential Natural Environments Based on Pedological Properties in the Coastal Conurbation of Subtropical Southeast Florida', Journal of Coastal Research, vol. 23, no. 2, pp. 319-351.
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Much of the coastal southeast Florida Peninsula is heavily urbanized, and many native environments have been destroyed or severely affected by urbanization. In an attempt to reconstruct potential natural environments, soil properties were used as a basis for recognizing prior landscapes. Pedological associations were adopted because they provided landscape features that were least modified by urbanization and they could be used as a unifying basis for categorization of native coastal environments. A geographic information system analysis ring was used for querying coverages for soil distribution patterns, vegetation, hydrology (drainage), topography, and geology. By simultaneously generalizing and recombining soil survey data, land use cover types, topography, and hydrology, it was possible to identify recurring patterns of soils, landforms, and vegetation. Results of this analysis produced 13 land systems with 25 subunits that included various types of lakebeds, tree islands, marshes, prairies, sloughs, sandplains, glades, marshlands, lagoons, estuaries, and beaches. The resulting map for potential natural environments in Palm Beach and Broward Counties complements conceptual ecological models for the Greater Florida Everglades Ecosystem, developed for the Comprehensive Everglades Restoration Plan. The Australian land systems approach, as applied in this study, provides insight into coastal environments that lie outside of ecological models for mostly protected areas inland.
Giordano, M, Norici, A, Gilmour, DJ & Raven, JA 2007, 'Physiological responses of the green alga Dunaliella parva (Volvocales, Chlorophyta) to controlled incremental changes in the N source', FUNCTIONAL PLANT BIOLOGY, vol. 34, no. 10, pp. 925-934.
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Gloag, RS, Ritchie, RJ, Chen, M, Larkum, AWD & Quinnell, RG 2007, 'Chromatic photoacclimation, photosynthetic electron transport and oxygen evolution in the Chlorophyll d-containing oxyphotobacterium Acaryochloris marina', Biochimica et Biophysica Acta - Bioenergetics, vol. 1767, no. 2, pp. 127-135.
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Changes in photosynthetic pigment ratios showed that the Chlorophyll d-dominated oxyphotobacterium Acaryochloris marina was able to photoacclimate to different light regimes. Chl d per cell were higher in cultures grown under low irradiance and red or green light compared to those found when grown under high white light, but phycocyanin/Chl d and carotenoid/Chl d indices under the corresponding conditions were lower. Chl a, considered an accessory pigment in this organism, decreased respective to Chl d in low irradiance and low intensity non-white light sources. Blue diode PAM (Pulse Amplitude Modulation) fluorometry was able to be used to measure photosynthesis in Acaryochloris. Light response curves for Acaryochloris were created using both PAM and O2 electrode. A linear relationship was found between electron transport rate (ETR), measured using a PAM fluorometer, and oxygen evolution (net and gross photosynthesis). Gross photosynthesis and ETR were directly proportional to one another. The optimum light for white light (quartz halogen) was about 206 ± 51 μmol m- 2 s- 1 (PAR) (Photosynthetically Active Radiation), whereas for red light (red diodes) the optimum light was lower (109 ± 27 μmol m- 2 s- 1 (PAR)). The maximum mean gross photosynthetic rate of Acaryochloris was 73 ± 7 μmol mg Chl d- 1 h- 1. The gross photosynthesis/respiration ratio (Pg/R) of Acaryochloris under optimum conditions was about 4.02 ± 1.69. The implications of our findings will be discussed in relation to how photosynthesis is regulated in Acaryochloris. © 2007 Elsevier B.V. All rights reserved.
Hill, R & Ralph, PJ 2007, 'Post-bleaching viability of expelled zooxanthellae from the scleractinian coral Pocillopora damicornis', Marine Ecology Progress Series, vol. 352, pp. 137-144.
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Coral bleaching events have been linked to elevated seawater temperatures in combination with intense light and can be characterised by the loss of symbionts (zooxanthellae, genus Symbiodinium) from the host tissue, as well as a reduction in photosynthetic pigments in these zooxanthellae. The long-term (days) viability of expelled zooxanthellae in the water column from the scleractiman coral Pocillopora damicornis was explored in this study through measurements of photosynthetic health and morphological condition. After initial expulsion, zooxanthellae were found to be photosynthetically competent and structurally intact. However, within 6 to 12 h following this time, photosystem II photochemical efficiency dramatically declined in these cells and photosynthetic damage was gradually manifested in the loss of structural integrity of the cell. The time of expulsion during bleaching exposure, as well as ambient water temperature, greatly influenced survivorship. Expelled zooxanthellae were collected at 4 different time intervals (0-6, 6-12, 12-24 and 24-36 h) following the onset of exposure to bleaching conditions (32 degrees C and 400 mu mol photons m(-2) s(-1)) and then maintained at 28, 30 or 32 degrees C and 100 mu mol photons m(-2) s(-1) for up to 96 h. Those cells expelled within the first 6 h of bleaching and held at 28 degrees C (lagoon temperature) had the greatest longevity, although even in this treatment, long-term photosynthetic viability was restricted to 5 d in the water column. This suggests that unless expelled zooxanthellae inhabit other environments of coral reefs (such as sediments) which may be more favourable for survival, their capacity for persistence in the environment is extremely limited.
Huang, EP, Marquis, CP & Gray, PP 2007, 'Development ofSuper-CHO protein-free medium based on a statistical design', Journal of Chemical Technology & Biotechnology, vol. 82, no. 5, pp. 431-441.
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Jensen, SI, Kuhl, M & Prieme, A 2007, 'Different bacterial communities associated with the roots and bulk sediment of the seagrass Zostera marina', F E M S Microbiology Ecology, vol. 62, no. 1, pp. 108-177.
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The bacterial community of Zostera marina-inhabited bulk sediment vs. root-associated bacteria was investigated by terminal restriction fragment length polymorphism and sequencing, and the spatial extension of the oxygen loss from roots was determined by oxygen microsensors. Extensive oxygen loss was found in the tip region of the youngest roots, and most of the rhizoplane of Z. marina roots was thus anoxic. A significant difference between the bacterial communities associated with the roots and bulk sediment was found. No significant differences were found between differently aged root-bundles. Terminal restriction fragments (TRFs) assigned to sulfate-reducing Deltaproteobacteria showed a relative mean distribution of 12% and 23% of the PCR-amplified bacterial community in the bulk-sediment at the two sites, but only contributed <2% to the root-associated communities. TRFs assigned to Epsilonproteobacteria showed a relative mean distribution of between 5% and 11% in the root-associated communities of the youngest root bundle, in contrast to the bulk-sediment where this TRF only contributed <1.3%. TRFs assigned to Actinobacteria and Gammaproteobacteria also seemed important first root-colonizers, whereas TRFs assigned to Deltaproteobacteria became increasingly important in the root-associated community of the older root bundles. The presence of the roots thus apparently selects for a distinct bacterial community, stimulating the growth of potential symbiotic Epsilon- and Gammaproteobacteria and/or inhibiting the growth of sulfate-reducing Deltaproteobacteria.
Jones, M, McManus, DP, Sivadorai, P, Glanfield, A, Moertel, L, Belli, SI & Gobert, G 2007, 'Tracking The Fate Of Iron In Early Development Of Human Blood Flukes', International Journal Of Biochemistry & Cell Biology, vol. 39, no. 9, pp. 1646-1658.
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Iron (Fe) is an important trace element found in nearly all organisms, and is used as a cofactor in many biological reactions. One role for Fe in some invertebrates is in stabilization of extracellular matrices. The human blood fluke, Schistosoma japonic
Jones, MR, Leith, ID, Fowler, D, Raven, JA, Sutton, MA, Nemitz, E, Cape, JN, Sheppard, LJ, Smith, RI & Theobald, MR 2007, 'Concentration-dependent NH3 deposition processes for mixed moorland semi-natural vegetation', Atmospheric Environment, vol. 41, no. 10, pp. 2049-2060.
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Dry deposition modelling typically assumes that canopy resistance (Rc) is independent of ammonia (NH3) concentration. An innovative flux chamber system was used to provide accurate continuous measurements of NH3 deposition to a moorland composed of a mixture of Calluna vulgaris (L.) Hull, Eriophorum vaginatum L. and Sphagnum spp. Ammonia was applied at a wide range of concentrations (1-100 μg m-3). The physical and environmental properties and the testing of the chamber are described, as well as results for the moorland vegetation using the 'canopy resistance' and 'canopy compensation point' interpretations of the data. Results for moorland plant species demonstrate that NH3 concentration directly affects the rate of NH3 deposition to the vegetation canopy, with Rc and cuticular resistance (Rw) increasing with increasing NH3 concentrations. Differences in Rc were found between night and day: during the night Rc increases from 17 s m-1 at 10 μg m-3 to 95 s m-1 at 80 μg m-3, whereas during the day Rc increases from 17 s m-1 at 10 μg m-3 to 48 s m-1 at 80 μg m-3. The lower resistance during the day is caused by the stomata being open and available as a deposition route to the plant. Rw increased with increasing NH3 concentrations and was not significantly different between day and night (at 80 μg m-3 NH3 day Rw=88 s m-1 and night Rw=95 s m-1). The results demonstrate that assessments using fixed Rc will over-estimate NH3 deposition at high concentrations (over ∼15 μg m-3). © 2006 Elsevier Ltd. All rights reserved.
Jones, MR, Leith, ID, Fowler, D, Raven, JA, Sutton, MA, Nemitz, E, Cape, JN, Sheppard, LJ, Smith, RI & Theobald, MR 2007, 'Concentration-dependent NH3 deposition processes for mixed moorland semi-natural vegetation', ATMOSPHERIC ENVIRONMENT, vol. 41, no. 10, pp. 2049-2060.
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Jones, MR, Leith, ID, Raven, JA, Fowler, D, Sutton, MA, Nemitz, E, Cape, JN, Sheppard, LJ & Smith, RI 2007, 'Concentration-dependent NH3 deposition processes for moorland plant species with and without stomata', ATMOSPHERIC ENVIRONMENT, vol. 41, no. 39, pp. 8980-8994.
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Kuhl, M, Rickelt, LF & Thar, R 2007, 'Combined Imaging of Bacteria and Oxygen in Biofilms', Applied and Environmental Microbiology, vol. 73, no. 19, pp. 6289-6295.
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Transparent sensors for microscopic O2 imaging were developed by spin coating an ultrathin (<1- to 2-µm) layer of a luminescent O2 indicator onto coverslips. The sensors showed (i) an ideal Stern-Volmer quenching behavior of the luminescence lifetime towards O2 levels, (ii) homogeneous measuring characteristics over the sensor surface, and (iii) a linear decline of luminescence lifetime with increasing temperature. When a batch of such coverslip sensors has been characterized, their use is thus essentially calibration free at a known temperature. The sensors are easy to use in flow chambers and other growth devices used in microbiology. We present the first application for combined imaging of O2 and bacteria in a biofilm flow chamber mounted on a microscope equipped with a spinning-disk confocal unit and a luminescence lifetime camera system.
Larkum, A, Ip, C, Chen, M & Jermiin, L 2007, 'Phylogeny of Prochlorococcus marinus: how is this species related to the rest of the Cyanobacteria?', PHOTOSYNTHESIS RESEARCH, vol. 91, no. 2-3, pp. 272-273.
Larkum, A, Lockhart, P & Howe, C 2007, 'The origin of plastids: A shopping bag model', PHOTOSYNTHESIS RESEARCH, vol. 91, no. 2-3, pp. 272-272.
Larkum, A, Zhang, Y, Churdh, B & Jermiin, L 2007, 'Modelling the IsiA-PSI supermolecular complex of cyanobacteria', PHOTOSYNTHESIS RESEARCH, vol. 91, no. 2-3, pp. 172-172.
Larkum, AWD, Lockhart, PJ & Howe, CJ 2007, 'Shopping for plastids', TRENDS IN PLANT SCIENCE, vol. 12, no. 5, pp. 189-195.
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Lee, RS, Pritchard, TR, Ajani, PA & Black, KP 2007, 'The influence of the East Australian Current eddy field on phytoplankton dynamics in the coastal zone.', Journal of Coastal Research: an international forum for the littoral sciences, no. 50, pp. 576-584.
Leegood, R, Roberts, K, Granum, E & Raven, J 2007, 'Single-cell C-4 photosynthesis in marine diatoms?', PHOTOSYNTHESIS RESEARCH, vol. 91, no. 2-3, pp. 222-223.
Lepetit, B, Volke, D, Szabo, M, Hoffmann, R, Garab, G, Wilhelm, C & Goss, R 2007, 'Spectroscopic and molecular characterization of the oligomeric antenna of the diatom Phaeodactylum tricornutum', Biochemistry, vol. 46, no. 34, pp. 9813-9822.
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The photosynthetic antenna system of diatoms contains fucoxanthin chlorophyll a/c binding proteins (FCPs), which are membrane intrinsic proteins showing high homology to the light harvesting complexes (LHC) of higher plants. In the present study, we used a mild solubilization of P. tricornutum thylakoid membranes in combination with sucrose density gradient centrifugation or gelfiltration and obtained an oligomeric FCP complex (FCPo). The spectroscopic characteristics and pigment stoichiometries of the FCPo complex were comparable to FCP complexes that were isolated after solubilization with higher detergent per chlorophyll ratios. The excitation energy transfer between the FCP-bound pigments was more efficient in the oligomeric FCPo complexes, indicating that these complexes may represent the native form of the diatom antenna system in the thylakoid membrane. Determination of the molecular masses of the two different FCP fractions by gelfiltration revealed that the FCP complexes consisted of trimers, whereas the FCPo complexes were either composed of six monomers or two tightly associated trimers. In contrast to vascular plants, stable functional monomers could not be isolated in P. tricornutum. Both types of FCP complexes showed two protein bands in SDS-gels with apparent molecular masses of 18 and 19 kDa, respectively. Sequence analysis by MS/MS revealed that the 19 kDa protein corresponded to the fcpC and fcpD genes, whereas the 18 kDa band contained the protein of the fcpE gene. The presence of an oligomeric antenna in diatoms is in line with the oligomeric organization of antenna complexes in different photoautotrophic groups.
Magnusson, SH, Fine, M & Kuhl, M 2007, 'Light microclimate of endolithic phototrophs in the scleractinian corals Montipora monasteriata and Porites cylindrica', Marine Ecology Progress Series, vol. 332, no. 1, pp. 119-128.
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The light microclimate of phototrophic endoliths growing within the scleractinian corals Porites cylindrica and Montipora monasteriata was described by scalar irradiance microprobe measurements within different layers of the coral skeleton. Characterisation of the pigments in individual layers was done by reflectance spectroscopy with fibre-optic radiance microprobes. The spectral measurements showed the presence of an endolithic community largely comprised of the green alga Ostreobium sp. within a 1 to 2 mm thick green band 2 to 6 mm below the coral surface. Additionally, spectral signatures of cyanobacteria and anoxygenic phototrophic bacteria were detected both in the coral tissue-containing top layer and within the skeleton matrix. The light microclimate within the coral skeleton was extremely poor in visible light but enriched in far-red wavelengths. Only a fraction of the incident photosynthetically available radiation (PAR, 400 to 700 nm) penetrated the coral tissue-containing layer, wherein 90 to 99% of the incident irradiance was attenuated due to intense scattering and absorption.
McMinn, A, Ryan, KG, Ralph, PJ & pankowski, A 2007, 'Spring sea ice photosynthesis, primary productivity and biomass distribution in eastern Antarctica, 2002-2004', Marine Biology, vol. 151, no. 3, pp. 985-995.
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While it is known that Antarctic sea ice biomass and productivity are highly variable over small spatial and temporal scales, there have been very few measurements from eastern Antarctic. Here we attempt to quantify the biomass and productivity and relat
Murray, SA, de Salas, M, Luong-Van, J & Hallegraeff, G 2007, 'Phylogenetic study of Gymnodinium dorsalisulcum comb. nov. from tropical Australian coastal waters (Dinophyceae)', Phycological Research, vol. 55, no. 2, pp. 176-184.
Murray, SA, Nagahama, Y & Fukuyo, Y 2007, 'Phylogenetic study of benthic, spine-bearing prorocentroids, including Prorocentrum fukuyoi sp. nov.', Phycological Research, vol. 55, pp. 91-102.
Pernice, M, Destoumieux-Garzon, D, Peduzzi, J, Rebuffat, S & Boucher-Rodoni, R 2007, 'Identification of a Vibrio strain producing antimicrobial agents in the excretory organs of Nautilus pompilius (Cephalopoda : Nautiloidea)', REVIEWS IN FISH BIOLOGY AND FISHERIES, vol. 17, no. 2-3, pp. 197-205.
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Pernice, M, Pichon, D, Domart-Coulon, I, Favet, J & Boucher-Rodoni, R 2007, 'Primary co-culture as a complementary approach to explore the diversity of bacterial associations in marine invertebrates: the example of Nautilus macromphalus (Cephalopoda : Nautiloidea)', MARINE BIOLOGY, vol. 150, no. 5, pp. 749-757.
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Pernice, M, Wetzel, S, Gros, O, Boucher-Rodoni, R & Dubilier, N 2007, 'Enigmatic dual symbiosis in the excretory organ of Nautilus macromphalus (Cephalopoda : Nautiloidea)', PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, vol. 274, no. 1614, pp. 1143-1152.
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Ralph, PJ, Durako, M, Enriquez, S, Collier, C & Doblin, MA 2007, 'Impact of light limitation on seagrasses', Journal of Experimental Marine Biology and Ecology, vol. 350, no. 1-2, pp. 176-193.
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Seagrass distribution is controlled by light availability, especially at the deepest edge of the meadow. Light attenuation due to both natural and anthropogenically-driven processes leads to reduced photosynthesis. Adaptation allows seagrasses to exist under these sub-optimal conditions. Understanding the minimum quantum requirements for growth (MQR) is revealed when light conditions are insufficient to maintain a positive carbon balance, leading to a decline in seagrass growth and distribution. Respiratory demands of photosynthetic and non-photosynthetic tissues strongly influence the carbon balance, as do resource allocations between above- and below-ground biomass. Seagrass light acclimation occurs on varying temporal scales, as well as across spatial scales, from the position along a single leaf blade to within the canopy and finally across the meadow. Leaf absorptance is regulated by factors such as pigment content, morphology and physical properties. Chlorophyll content and morphological characteristics of leaves such as leaf thickness change at the deepest edge. We present a series of conceptual models describing the factors driving the light climate and seagrass responses under current and future conditions, with special attention on the deepest edge of the meadow.
Ralph, PJ, Larkum, A & Kuhl, M 2007, 'Photobiology of endolithic microorganisms in living coral skeletons: 1. Pigmentation, spectral reflectance and variable chlorophyll fluorescence analysis of endoliths in the massive corals Cyphastrea serailia, Porites lutea and Goniastrea australensi', Marine Biology, vol. 152, no. 2, pp. 395-404.
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We used microscopy, reflectance spectroscopy, pigment analysis, and photosynthesis-irradiance curves measured with variable fluorescence techniques to characterise the endolithic communities of phototrophic microorganisms in the skeleton of three massive
Ralph, PJ, Ryan, KG, Martin, AJ & Fenton, G 2007, 'Melting out of sea ice causes greater photosynthetic stress in algae than freezing in', Journal Of Phycology, vol. 43, no. 5, pp. 948-956.
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Sea ice is the dominant feature of polar oceans and contains significant quantities of microalgae. When sea ice forms and melts, the microalgal cells within the ice matrix are exposed to altered salinity and irradiance conditions, and subsequently, their
Ralph, PJ, Smith, RA, Macinnis-Ng, CM & Seery, CR 2007, 'Use of fluorescence-based ecotoxicological bioassays in monitoring toxicants and pollution in aquatic systems: Review', Toxicological and Environmental Chemistry, vol. 89, no. 4, pp. 631-649.
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Chlorophyll a fluorescence has the potential to become a valuable ecotoxicological endpoint, which could be used with a range of aquatic phototrophs. Chlorophyll a fluorescence bioassays have been applied in the assessment of heavy metals, herbicides, petrochemicals and nutrients. The strengths of this endpoint are that it is rapid, non-invasive and non-destructive, while the major weakness is the lack of clear ecological relevance. We provide an overview of chlorophyll a fluorescence applications in ecotoxicology. We reviewed test conditions, parameters and protocols used to date and found standardised protocols to be lacking. The most favoured fluorescence parameters were maximum quantum yield (Fv/Fm) and effective quantum yield (?PSII); microalgae were the most widely used tested organism, herbicides the most commonly tested toxicant, while most studies lacked a summary statistic (such as EC50). We recommend that future research in aquatic chlorophyll a fluorescence ecotoxicology focus on standardisation of test protocols and statistical techniques.
Raven, J 2007, 'Astrobiology: Photosynthesis in watercolours', Nature, vol. 448, no. 7152, p. 418.
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The spectrum of stellar radiation available to an organism is altered by the atmosphere and water on the planet it inhabits. Study of this relationship can outline the limits to photosynthesis. ©2007 Nature Publishing Group.
Raven, J 2007, 'Why do phytoplankton make such a Fuss about carbon dioxide?', JOURNAL OF PHYCOLOGY, vol. 43, pp. 54-55.
Raven, J & Crane, P 2007, 'Trees', Current Biology, vol. 17, no. 9.
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Raven, J, Beardall, J, Franklin, L & Giordano, M 2007, 'Influence of nutrient supply and UV on photosynthesis and inorganic carbon concentrating mechanisms: relation to environmental change?', PHOTOSYNTHESIS RESEARCH, vol. 91, no. 2-3, pp. 297-297.
Raven, JA & Larkum, AWD 2007, 'Are there ecological implications for the proposed energetic restrictions on photosynthetic oxygen evolution at high oxygen concentrations?', PHOTOSYNTHESIS RESEARCH, vol. 94, no. 1, pp. 31-42.
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Roberts, K, Granum, E, Leegood, RC & Raven, JA 2007, 'C-3 and C-4 pathways of photosynthetic carbon assimilation in marine diatoms are under genetic, not environmental, control', PLANT PHYSIOLOGY, vol. 145, no. 1, pp. 230-235.
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Roberts, K, Granum, E, Leegood, RC & Raven, JA 2007, 'Carbon acquisition by diatoms', PHOTOSYNTHESIS RESEARCH, vol. 93, no. 1-3, pp. 79-88.
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Roeselers, G, Norris, TB, Castenholz, RW, Rysgaard, S, Glud, RN, Kuhl, M & Muyzer, G 2007, 'Diversity of phototrophic bacteria in microbial mats from Arctic hot springs (Greenland)', Environmental Microbiology, vol. 9, no. 1, pp. 26-38.
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We investigated the genotypic diversity of oxygenic and anoxygenic phototrophic microorganisms in microbial mat samples collected from three hot spring localities on the east coast of Greenland. These hot springs harbour unique Arctic microbial ecosystems that have never been studied in detail before. Specific oligonucleotide primers for cyanobacteria, purple sulfur bacteria, green sulfur bacteria and Choroflexus/Roseiflexus-like green non-sulfur bacteria were used for the selective amplification of 16S rRNA gene fragments. Amplification products were separated by denaturing gradient gel electrophoresis (DGGE) and sequenced. In addition, several cyanobacteria were isolated from the mat samples, and classified morphologically and by 16S rRNA-based methods. The cyanobacterial 16S rRNA sequences obtained from DGGE represented a diverse, polyphyletic collection of cyanobacteria. The microbial mat communities were dominated by heterocystous and non-heterocystous filamentous cyanobacteria. Our results indicate that the cyanobacterial community composition in the samples were different for each sampling site. Different layers of the same heterogeneous mat often contained distinct and different communities of cyanobacteria. We observed a relationship between the cyanobacterial community composition and the in situ temperatures of different mat parts. The Greenland mats exhibited a low diversity of anoxygenic phototrophs as compared with other hot spring mats which is possibly related to the photochemical conditions within the mats resulting from the Arctic light regime.
Santabarbara, S, Chen, M, Larkum, AWD & Evans, MCW 2007, 'An electron paramagnetic resonance investigation of the electron transfer reactions in the chlorophyll d containing photosystem I of Acaryochloris marina', FEBS Letters, vol. 581, no. 8, pp. 1567-1571.
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Electron paramagnetic resonance (EPR) spectroscopy reveals functional and structural similarities between the reaction centres of the chlorophyll d-binding photosystem I (PS I) and chlorophyll a-binding PS I. Continuous wave EPR spectrometry at 12 K identifies iron-sulphur centres as terminal electron acceptors of chlorophyll d-binding PS I. A transient light-induced electron spin echo (ESE) signal indicates the presence of a quinone as the secondary electron acceptor (Q) between P740+ and the iron-sulphur centres. The distance between P740+ and Q- was estimated within point-dipole approximation as 25.23 ± 0.05 Å, by the analysis of the electron spin echo envelope modulation. © 2007 Federation of European Biochemical Societies.
Seebacher, F & Murray, SA 2007, 'Transient receptor potential ion channels control thermoregulatory behaviour in reptiles', PLoS One, vol. 3, p. e281.
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Seuront, L, Lacheze, C, Doubell, MJ, Seymour, JR, Dongen-Vogels, VV, Newton, K, Alderkamp, AC & Mitchell, JG 2007, 'The influence of Phaeocystis globosa on microscale spatial patterns of chlorophyll a and bulk-phase seawater viscosity', Biogeochemistry, vol. 83, no. 1-3, pp. 173-188.
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A two-dimensional microscale (5 cm resolution) sampler was used over the course of a phytoplankton spring bloom dominated by Phaeocystis globosa to investigate the structural properties of chlorophyll a and seawater excess viscosity distributions. The microscale distribution patterns of chlorophyll a and excess viscosity were never uniform nor random. Instead they exhibited different types and levels of aggregated spatial patterns that were related to the dynamics of the bloom. The chlorophyll a and seawater viscosity correlation patterns were also controlled by the dynamics of the bloom with positive and negative correlations before and after the formation of foam in the turbulent surf zone. The ecological relevance and implications of the observed patchiness and biologically induced increase in seawater viscosity are discussed and the combination of the enlarged colonial form and mucus secretion is suggested as a competitive advantage of P. globosa in highly turbulent environments where this species flourishes.
Seymour, JR, Humphreys, WF & Mitchell, JG 2007, 'Stratification of the microbial community inhabiting an anchialine sinkhole', Aquatic Microbial Ecology, vol. 50, pp. 11-24.
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Bundera Sinkhole in northwestern Australia is an anchialine ecosystem characterised by a highly stratified water column comprising a complex polymodal profile of several physico-chemical parameters. We studied the microscale and finescale dynamics of the resident microbial community within the sinkhole. Sub-millimetre scale distributions of phytoplankton abundance were measured in the top 8 m of the water column using a free-falling high resolution fluorometer. Depth profiles were characterised by a strong, 10 to 20 cm layer of elevated fluorescence, occurring at approximately 1 m depth, which despite changes in magnitude and width was found to persist during a 24 h sampling period. Near surface distributions of microbial populations were measured using a syringe sampling profiler, which allowed for collection of water samples at 5 cm resolution, and flow cytometric analysis. These samples revealed a complex microbial assemblage, with multiple sub-populations of viruses, bacteria and picophytoplankton present throughout the water column. Within 3 m profiles, the bacterial and virus populations showed marked shifts in relative abundance, with changes of over 35-fold observed across as little as 20 cm. Samples collected from the surface to a depth of 30 m by divers also revealed distinct peaks and layers in the relative abundance of the different bacteria and virus sub-populations, which often corresponded to heterogeneities in chemical and nutrient parameters, and at some depths indicated the prevalence of chemolithotrophic populations. The complex patterns described here represent the first comprehensive observations of microbial spatiotemporal dynamics throughout an anchialine ecosystem and reveal a highly structured microbial habitat consisting of discrete niches, each dominated by heterotrophic, phototrophic or chemoautotrophic microorganisms.
Seymour, JR, Marcos, M & Stocker, R 2007, 'Chemotactic Response of Marine Micro-Organisms to Micro-Scale Nutrient Layers', Journal of Visualized Experiments, vol. 4, pp. 1-3.
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The degree to which planktonic microbes can exploit microscale resource patches will have considerable implications for oceanic trophodynamics and biogeochemical flux. However, to take advantage of nutrient patches in the ocean, swimming microbes must overcome the influences of physical forces including molecular diffusion and turbulent shear, which will limit the availability of patches and the ability of bacteria to locate them. Until recently, methodological limitations have precluded direct examinations of microbial behaviour within patchy habitats and realistic small-scale flow conditions. Hence, much of our current knowledge regarding microbial behaviour in the ocean has been procured from theoretical predictions. To obtain new information on microbial foraging behaviour in the ocean we have applied soft lithographic fabrication techniques to develop 2 microfluidic devices, which we have used to create (i) microscale nutrient patches with dimensions and diffusive characteristics relevant to oceanic processes and (ii) microscale vortices, with shear rates corresponding to those expected in the ocean. These microfluidic devices have permitted a first direct examination of microbial swimming and chemotactic behaviour within a heterogeneous and dynamic seascape. The combined use of epifluorescence and phase contrast microscopy allow direct examinations of the physical dimensions and diffusive characteristics of nutrient patches, while observing the population-level aggregative response, in addition to the swimming behaviour of individual microbes. These experiments have revealed that some species of phytoplankton, heterotrophic bacteria and phagotrophic protists are adept at locating and exploiting diffusing microscale resource patches within very short time frames.
Seymour, JR, Seuront, L & Mitchell, JG 2007, 'Microscale gradients of planktonic microbial communities above the sediment surface in a mangrove estuary', ESTUARINE, COASTAL AND SHELF SCIENCE, vol. 73, no. 3-4, pp. 651-666.
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The microscale (1 and 4 cm sampling resolution) distributions of chemical (O2, NH3, NO3-, NO2-, PO43-) and biological (Chl a, phytoplankton, bacterioplankton, viruses) parameters were measured in the 16 cm of water immediately overlaying the sediment-water interface (SWI) within a temperate mangrove estuary in South Australia during December 2003 and March 2004. Shear velocities (u*) during the time of sampling were very low (<0.1 cm s-1), and we consequently predict that resuspension of organisms and materials was negligible. In December 2003, profiles were often characterised by strong gradients in nutrients and organisms, with the highest concentrations often observed within 0.5 cm of the SWI. Microscale patterns in O2, NH3, NO3- and NO2- indicated that a variety of anaerobic and aerobic transformation processes probably occurred at the SWI and within profiles. Strong gradients in PO43- were indicative of nutrient flux across the SWI as a consequence of degradation processes in the sediments. Pico- and nanophytoplankton concentrations were strongly correlated (p < 0.01) to PO43-, and exhibited 12- and 68-fold changes in abundance, respectively, with highest concentrations observed nearest to the SWI. Several bacterial subpopulations were discriminated using flow cytometry and significant shifts in the `cytometric structure of the bacterial community were observed within microscale profiles.
Siboni, N, Lidor, M, Kramarsky-Winter, E & Kushmaro, A 2007, 'Conditioning film and initial biofilm formation on ceramics tiles in the marine environment', FEMS MICROBIOLOGY LETTERS, vol. 274, no. 1, pp. 24-29.
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Six, C, Thomas, J-C, Garczarek, L, Ostrowski, M, Dufresne, A, Blot, N, Scanlan, DJ & Partensky, F 2007, 'Diversity and evolution of phycobilisomes in marine Synechococcus spp.: a comparative genomics study', GENOME BIOLOGY, vol. 8, no. 12.
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Staal, M, Thar, R, Kuhl, M, van Loosdrecht, MC, Wolf, G, de Brouwer, JF & Rijstenbil, JW 2007, 'Different carbon isotope fractionation patterns during the development of phototrophic freshwater and marine biofilms', Biogeosciences, vol. 4, no. 4, pp. 613-626.
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Natural phototrophic biofilms are influenced by a broad array of abiotic and biotic factors and vary over temporal and spatial scales. Different developmental stages can be distinguished and growth rates will vary due to the thickening of the biofilm, which is expected to lead to a limitation of light or mass transport. This study shows that variation in CO2(aq) availability leads to a fractionation shift and thereby affects d13C signatures during biofilm development. For phototrophic freshwater biofilms it was found that the d13C value became less negative with the thickening of the biofilm, while the opposite trend was found in marine biofilms. Modeling and pH profiling indicated that the trend in the freshwater system was caused by an increase in CO2(aq) limitation resulting in an increase of HCO3- as C-source. The opposite trend in the marine system could be explained by a higher heterotrophic biomass and activity causing a higher carbon recycling and thereby lower d13C values. We conclude that d13C was more related to the net areal photosynthesis rate and carbon recycling, rather than to the growth rate of the biofilms.
Suggett, DJ, Le Floc'h, E, Harris, GN, Leonardos, N & Geider, RJ 2007, 'Different strategies of photoacclimation by two strains of Emiliania huxleyi (Haptophyta)', Journal Of Phycology, vol. 43, no. 6, pp. 1209-1222.
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Photoacclimation involves the modification of components of the light and dark reactions to optimize photosynthesis following changes in available light. All of the energy required for photosynthesis comes from linear electron transport through PSII and
Ulstrup, K, Kuhl, M & Bourne, DG 2007, 'Zooxanthellae Harvested by Ciliates Associated with Brown Band Syndrome of Corals Remain Photosynthetically Competent', Applied and Environmental Microbiology, vol. 73, no. 11, pp. 1968-1975.
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Brown band syndrome is a new coral affliction characterized by a local accumulation of yet-unidentified ciliates migrating as a band along the branches of coral colonies. In the current study, morphologically intact zooxanthellae (= Symbiodinium) were observed in great numbers inside the ciliates (>50 dinoflagellates per ciliate). Microscale oxygen measurements and variable chlorophyll a fluorescence analysis along with microscopic observations demonstrated that zooxanthellae within the ciliates are photosynthetically competent and do not become compromised during the progression of the brown band zone. Zooxanthellae showed similar trends in light acclimation in a comparison of rapid light curve and steady-state light curve measures of variable chlorophyll a fluorescence. Extended light exposure of steady-state light curves resulted in higher quantum yields of photosystem II. The brown band tissue exhibited higher photosynthetically active radiation absorptivity, indicating more efficient light absorption due to a higher density of zooxanthellae in the ciliate-dominated zone. This caused relatively higher gross photosynthesis rates in the zone with zooxanthella-containing ciliates compared to healthy coral tissue. The observation of photosynthetically active intracellular zooxanthellae in the ciliates suggests that the latter can benefit from photosynthates produced by ingested zooxanthellae and from photosynthetic oxygen production that alleviates diffusion limitation of oxic respiration in the densely populated brown band tissue.
Ulstrup, K, Ralph, PJ, van Oppen, MJ & Kuhl, M 2007, 'Inter-polyp genetic and physiological characterisation of Symbiodinium in an Acropora valida colony', Marine Biology: international journal on life in oceans and coastal waters, vol. 153, no. 2, pp. 225-234.
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Corals harbouring genetically mixed communities of endosymbiotic algae (Symbiodinium) often show distribution patterns in accordance with differences in light climate across an individual colony. However, the physiology of these genetically characterised communities is not well understood. Single stranded conformation polymorphism (SSCP) and real time quantitative polymerase chain reaction (qPCR) analyses were used to examine the genetic diversity of the Symbiodinium community in hospite across an individual colony of Acropora valida at the spatial scale of single polyps. The physiological characteristics of the polyps were examined prior to sampling with a combined O2 microelectrode with a fibre-optic microprobe (combined sensor diameter 50-100 μm) enabling simultaneous measurements of O2 concentration, gross photosynthesis rate and photosystem II (PSII) quantum yield at the coral surface as a function of increasing irradiances. Both sun- and shade-adapted polyps were found to harbour either Symbiodinium clade C types alone or clades A and C simultaneously. Polyps were grouped in two categories according to (1) their orientation towardps light, or (2) their symbiont community composition. Physiological differences were not detected between sun- and shade-adapted polyps, but O2 concentration at 1,100 μmol photons m-2 s-1 was higher in polyps that harboured both clades A and C symbionts than in polyps that harboured clade C only. These results suggest that the acclimatisation of zooxanthellae of individual polyps of an A. valida colony to ambient light levels may not be the only determinant of the photosynthetic capacity of zooxanthellae. Here, we found that photosynthetic capacity is also likely to have a strong genetic basis and differs between genetically distinct Symbiodinium types.
Unnikrishnan, KP, Fathima, A, Hashim, KM & Balachandran, I 2007, 'Antioxidant studies and determination of wedelolactone in Eclipta alba', Journal of Plant Sciences, vol. 2, no. 4, pp. 459-464.
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The present research reports the results of the phytochemical studies carried out to identify the diagnostic features of the E. alba. A high performance thin layer chromatographic method was standardized to determine the wedelolactone content in whole plant of E. alba. Methanolic extracts of samples from three different sources were used for analysis. The mean assay of wedelolactone was of range 0.481-0.702 mg g-1 of drug powder. Radical scavenging activity of methanolic extract was evaluated by DPPH assay method superoxide radical scavenging activity in riboflavin/light/NBT system and nitric oxide radical scavenging activity in sodium nitroprusside/Griess reagent system. The assay results indicate that the DPPH, superoxide and nitric oxide scavenging activity were intense (19.25, 39.25 and 58.26 μg mL-1, respectively). The phytochemical features identified in the present study can be used as identification markers of this important analgesic agent. © 2007 Academic Journals Inc.
Zhang, Y, Chen, M, Zhou, BB, Jermiin, LS & Larkum, AWD 2007, 'Evolution of the inner light-harvesting antenna protein family of cyanobacteria, algae, and plants', Journal of Molecular Evolution, vol. 64, no. 3, pp. 321-331.
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Two hypotheses account for the evolution of the inner antenna light-harvesting proteins of oxygenic photosynthesis in cyanobacteria, algae, and plants: one in which the CP43 protein of photosytem II gave rise to the extrinsic CP43-like antennas of cyanobacteria (i.e. IsiA and Pcb proteins), as a late development, and the other in which CP43 and CP43-like proteins derive from an ancestral protein. In order to determine which of these hypotheses is most likely, we analyzed the family of antenna proteins by a variety of phylogenetic techniques, using alignments of the six common membrane-spanning helices, constructed using information on the antenna proteins' three-dimensional structure, and surveyed for evidence of factors that might confound inference of a correct phylogeny. The first hypothesis was strongly supported. As a consequence, we conclude that the ancestral photosynthetic apparatus, with 11 membrane-spanning helices, split at an early stage during evolution to form, on the one hand, the reaction center of photosystem II and, on the other hand, the ancestor of inner antenna proteins, CP43 (PsbC) and CP47 (PsbB). Only much later in evolution did the CP43 lineage give rise to the CP43' proteins (IsiA and Pcb) of cyanobacteria. © 2007 Springer Science+Business Media, Inc.