Publications

BOROWITZKA, MA & LARKUM, AWD 1976, 'Calcification in the Green AlgaHalimeda', Journal of Experimental Botany, vol. 27, no. 5, pp. 879-893.
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BOROWITZKA, MA & LARKUM, AWD 1976, 'Calcification in the Green AlgaHalimeda', Journal of Experimental Botany, vol. 27, no. 5, pp. 864-878.
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BOROWITZKA, MA & LARKUM, AWD 1976, 'Calcification in the Green AlgaHalimeda', Journal of Experimental Botany, vol. 27, no. 5, pp. 894-907.
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Coombe, RG & George, AM 1976, 'A rapid method for the purification of β-lactamase from Bacillus cereus by affinity chromatography', Analytical Biochemistry, vol. 75, no. 2, pp. 652-655.
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A procedure for the purification of β-lactamase from Bacillus cereus in a single chromatographic step is described. The enzyme is isolated from the crude culture supernatant by affinity chromatography. An inhibitor, methicillin, was immobilised by covalent attachment to the insoluble column gel, Sepharose. The enzyme was adsorbed to the column ligand from the crude supernatant and was subsequently released by increasing the ionic strength of the eluting buffer. In this way the enzyme was selectively isolated from other proteins in the crude supernatant. About 98% of the original β-lactamase activity was recovered in the purified enzyme fraction. © 1976.

Coombe, RG & George, AM 1976, 'An Alternative Coupling Procedure for Preparing Activated Sepharose for Affinity Chromatography of Penicillinase', Australian Journal of Biological Sciences, vol. 29, no. 4, pp. 305-305.
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Most applications of affinity chromatography employ the cyanogen bromide activation scheme first devised by Axtm et al. (1967). Porath and Sundberg (1972) reported an alternative procedure in which phloroglucinol and divinylsulphone are used in activating reactions. The advantages of this scheme and parameters relevant to the activating reactions are reported here. Conditions for the attachment of various ligand molecules to sepharose using a divinylsulphone activation method are defined, and a comparison with cyanogen bromide activating and coupling techniques is drawn. a-Chymotrypsin is immobilized by covalent attachment to activated sepharose. The optimum coupling pH is 8� 0-8� 6 and the reaction is virtually complete after 20 h at room temperature. Conjugates containing as much as 2 g of enzyme per gram dry weight of polymer were obtained. The immobilized enzyme retained 41 % of the free enzymic activity. An affinity column of divinylsulphone-activated methicillin-sepharose was used to demonstrate the reversible adsorption of penicillinase.

DOOLEY, AH 1976, 'SPECTRAL THEORY OF POSETS AND ITS APPLICATIONS TO C-STAR-ALGEBRAS', TRANSACTIONS OF THE AMERICAN MATHEMATICAL SOCIETY, vol. 224, no. 1, pp. 143-155.
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Dooley, AH 1976, 'The spectral theory of posets and its applications to 𝐶*-algebras', Transactions of the American Mathematical Society, vol. 224, no. 1, pp. 143-155.
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This paper uses methods from the spectral theory of partially ordered sets to clarify and extend some recent results concerning approximately finite-dimensional C ∗ {C^\ast } -algebras. An extremely explicit description is obtained of the Jacobson topology on the primitive ideal space, and it is shown that this topology has a basis of quasi-compact open sets. In addition, the main results of [4] are proved using only elementary means.

Gauntlett, DJ, Leslie, LM & Hincksman, DR 1976, 'A semi‐implicit forecast model using the flux form of the primitive equations', Quarterly Journal of the Royal Meteorological Society, vol. 102, no. 431, pp. 203-217.
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AbstractAs part of an attempt to implement more efficient integration procedures, a semi‐implicit time differencing procedure has been evaluated within the context of a southern hemisphere, six‐level primitive equations model. The model employs the flux form of the primitive equations and includes processes such as convection, vertical and horizontal diffusion, and orography.Comparative explicit/semi‐implicit integrations show only marginal meteorological differences. However, the semi‐implicit scheme allows a timestep about five times as large. This represents a net saving factor of approximately 2.5 for integrations involving no orography. In instances where high orography is included net efficiency advantages may be degraded significantly when using the flux form of the primitive equations unless special numerical procedures are adopted during the solution of key model equations.On the basis of these successful results, the semi‐implicit scheme is now being used in the separate development of a limited‐area prediction model to provide routine forecasts for the Australasian region.

Jeffrey, PD, Milthorpe, BK & Nichol, LW 1976, 'Polymerization Pattern of Insulin at pH 7.0', Biochemistry, vol. 15, no. 21, pp. 4660-4665.
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Sedimentation equilibrium results, obtained with bovine zinc-free insulin (with and without a component of proinsulin) at pH 7.0/0.2, 25 °C, and up to a total concentration of 0.8 g/1., are shown to be consistent with three different polymerization patterns, all involving an isodesmic indefinite self-association of specified oligomeric species. The analysis procedure, based on closed solutions formed by summing infinite series, yields for each pattern a set of equilibrium constants. It is shown that a distinction between the possible patterns can be made by analyzing sedimentation equilibrium results obtained in a higher total concentration range (up to 4 g/1.) with insulin freed of zinc and proinsulin, account being taken of the composition dependence of activity coefficients. The favored pattern, which differs from that previously reported in the literature, involves the dimerization of monomeric insulin (mol wt 5734), governed by a dimerization constant of 11 × 104 M-1 and the isodesmic indefinite self-association of the dimer, described by an association constant of 1.7 ×104 M-1. This polymerization pattern is also shown to be consistent with the reaction boundary observed in sedimentation velocity experiments. © 1976, American Chemical Society. All rights reserved.

KALMAN, JR, FAIRWEATHER, RB, HVISTENDAHL, G & WILLIAMS, DH 1976, 'METHYL RADICAL EXPULSIONS OCCURRING WITH KINETIC-ENERGY RELEASE', JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, no. 15, pp. 604-605.
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Larkum, AWD 1976, 'Ecology of Botany Bay. I. Growth of Posidonia australis (Brown) Hook. f. in Botany Bay and other bays of the Sydney basin', Marine and Freshwater Research, vol. 27, no. 1, pp. 117-117.
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The distribution, biomass and density of various beds of P. australis have been determined for Botany Bay (N.S.W.). Comparisons have been made with sites at Broken Bay, Port Hacking and Jervis Bay and these include observations of other seagrass beds (Zostera capricorni, Heterozostera tasmanica, Halophila ovalis and Halophila decipiens). The P. australis plants observed were all of the broad-leaved (1.0-1.2 cm) variety. The length of leaves appears to be correlated with depth and with degree of exposure to wave action. The distribution of P. australis in Botany Bay is almost entirely restricted to the southern side and is limited to a maximum depth of - 3 m compared with -7 to -9 m at other sites. The growth of plants in well-established beds seems to be as vigorous in Botany Bay as elsewhere but flowering is poor and infrequent and the overgrowth of epiphytes is very great at certain seasons. Clearing experiments in Botany Bay indicate that recolonization by surrounding plants or seedlings is extremely slow. Transplant experiments indicate that mature plants will survive transplanting to cleared sites on both the northern and southern sides of the Bay. Evidence for a much more extensive distribution of P. australis in Botany Bay in times past and the possible effects of pollution are discussed.

Leitch, EC 1976, 'Emplacement of an epizonal granodiorite pluton λ by vertical block elevation', Geological Magazine, vol. 113, no. 6, pp. 553-560.
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SummaryThe Carrai Granodiorite is an intrusive magmatic pluton with steeply dipping margins surrounded by a well-developed contact metamorphic aureole. Emplacement of the body was accompanied by little distortion of neighbouring rocks and involved vertical displacement of pre-existing material. Scarcity of country rock blocks, and the presence of numerous dioritic xenoliths indicate that stoping was unimportant, and the pluton was accommodated in the upper crust by vertical uplift of earlier rocks (‘block elevation’). A close relationship existed between faulting and granodiorite emplacement. Deep shears possibly account for the generation of the linear zone of plutons in which the Granodiorite is located.

MADDOCKS, I, ANDERS, EM & DENNIS, E 1976, 'DONOVANOSIS IN PAPUA-NEW-GUINEA', BRITISH JOURNAL OF VENEREAL DISEASES, vol. 52, no. 3, pp. 190-196.

NICHOL, LW, JEFFREY, PD & MILTHORPE, BK 1976, 'ANALYSIS OF SEDIMENTATION EQUILIBRIUM RESULTS OBTAINED WITH INDEFINITELY SELF-ASSOCIATING SYSTEMS USING A PROCEDURE BASED ON LAPLACE TRANSFORMATION', JOURNAL OF PHYSICAL CHEMISTRY, vol. 80, no. 10, pp. 1071-1075.
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Equations are developed in closed form which permit the simulation of the distribution of total concentration vs. radial distance obtainable in the sedimentation equilibrium of a system of specified initial concentration undergoing indefinite self-association. Simulated distributions obtained with a variety of systems involving one or more equilibrium constants are used to test an analysis procedure which fits the distribution to a function capable of inverse Laplace transformation and leads to a specification of the relative amounts of the species in the cell as a function of their molecular weights. It is shown that such results may be related to the equilibrium concentrations of oligomeric forms at each radial distance, thereby permitting successive equilibrium constants appropriate to the indefinite self-association to be estimated.

NICHOL, LW, JEFFREY, PD & MILTHORPE, BK 1976, 'SEDIMENTATION EQUILIBRIUM OF HETEROGENEOUSLY ASSOCIATING SYSTEMS AND MIXTURES OF NON-INTERACTING SOLUTES - ANALYSIS WITHOUT DETERMINATION OF MOLECULAR-WEIGHT AVERAGES', BIOPHYSICAL CHEMISTRY, vol. 4, no. 3, pp. 259-267.
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Sedimentation equilibrium is first considered of a system in which a ligand of any size binds to an acceptor at p sites, the experimental result, obtained with either interference or absorption optics, being a distribution of total solute concentration as a function of radial distance. Theory illustrated by a numerical example, is presented which shows that this distribution may be analysed to give the activity of the unbound ligand as a function of total weight concentration. It is shown that this information may be used together with conservation of mass equations written in terms of the initial mixing composition to evaluate the equilibrium constant(s) relevant to the system. Correlation with composition evaluation by use of absorption optics (when possible) is also discussed. The procedure does not involve solution of simultaneous equations which are sums of exponentials nor differentiation of experimental results to obtain apparent weight-average molecular weights. It is general in that it leads to the evaluation of the activity of the species characterized by the smallest M(l-Image ?) product and, accordingly, is shown to be useful in the analysis of non-interacting as well as of interacting systems.

Novikov, AA 1976, 'Application of the method of coupled waves to an analysis of nonresonance interaction', Radiophysics and Quantum Electronics, vol. 19, no. 2, pp. 225-227.
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Smith, RK & Leslie, LM 1976, 'Thermally driven vortices: A numerical study with application to dust-devil dynamics', Quarterly Journal of the Royal Meteorological Society, vol. 102, no. 434, pp. 791-804.
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Zinder, YA & Portugal, VM 1976, 'Mathematical model of operative control of the course of production', Cybernetics, vol. 11, no. 2, pp. 264-270.
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